Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	172	67.8	2301510	98.9%	2276193	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,970,032	(G)_6→7	coding (1002/1047 nt)	wzzE →	enterobacterial common antigen polysaccharide co‑polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,970,026	1	.	G	87.0%	161.9 / 17.6	54	coding (996/1047 nt)	wzzE	enterobacterial common antigen polysaccharide co‑polymerase
Reads supporting (aligned to +/- strand): ref base . (0/7); new base G (30/17); total (30/24)
Fisher's exact test for biased strand distribution p-value = 1.95e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

GGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑C‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTAC  >  NC_000913/3969888‑3970158

ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑C‑g                                                                                                                                     >  2:912753/1‑140 (MQ=255)

ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑C‑g                                                                                                                                     >  2:54173/1‑140 (MQ=255)

     tGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑C‑gggggg                                                                                                                                >  1:566534/1‑140 (MQ=255)

     tGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑C‑gggggg                                                                                                                                >  1:369267/1‑140 (MQ=255)

      gTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑C‑ggggggg                                                                                                                               <  2:891758/140‑2 (MQ=255)

         aaaCACACTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑C‑gggggggctg                                                                                                                            >  2:72926/1‑136 (MQ=255)

            cacCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATc                                                                                                                          <  1:54173/140‑1 (MQ=255)

               ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCggg                                                                                                                       <  1:304934/140‑1 (MQ=255)

               ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCggg                                                                                                                       >  1:507183/1‑140 (MQ=255)

               ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCggg                                                                                                                       >  1:507182/1‑140 (MQ=255)

               ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑A‑CGGGGGGGCTGATCggg                                                                                                                       >  1:762062/1‑140 (MQ=255)

                 tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGc                                                                                                                     <  1:72926/140‑1 (MQ=255)

                         ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACTCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCg                                                                                                             >  1:63854/1‑140 (MQ=255)

                         ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGTCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCg                                                                                                             >  1:504674/1‑140 (MQ=255)

                         ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGAt                                                                                                                           >  1:1060403/1‑126 (MQ=255)

                         ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGAt                                                                                                                           <  2:1060403/126‑1 (MQ=255)

                         ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCg                                                                                                             >  2:105904/1‑140 (MQ=255)

                         ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCg                                                                                                             >  1:524356/1‑140 (MQ=255)

                             cAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCTTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCAtt                                                                                                         >  2:150150/1‑140 (MQ=255)

                                     gATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACccgcc                                                                                                 >  1:540672/1‑140 (MQ=255)

                                     gATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACccgcc                                                                                                 >  2:148097/1‑140 (MQ=255)

                                         cgcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTg                                                                                             >  1:602872/1‑140 (MQ=255)

                                           cgTTTTCAGACCTATCGCTTTTTGCGTCCGCCGGAAGAACGGGTAAACCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTACCCCGCCGTTGCt                                                                                           <  2:811301/140‑1 (MQ=255)

                                           cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt                                                                                           <  1:912753/140‑1 (MQ=255)

                                           cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt                                                                                           <  1:1105057/140‑1 (MQ=255)

                                           cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt                                                                                           >  1:509238/1‑140 (MQ=255)

                                            gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑TGG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc                                                                                          <  1:685344/140‑1 (MQ=255)

                                              tttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa                                                                                        <  2:507183/140‑1 (MQ=255)

                                                        ttCGCTTTTTGCGTACGCCGAAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGGGGGGCTTTG‑C‑CGGGGGGGCTGCGGGGGGTGGGTGTAGCATTAACCCGCCGTTGCTCGAAATAGCAaca                                                                              <  2:602872/139‑1 (MQ=255)

                                                         tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAacac                                                                             >  2:477040/1‑140 (MQ=255)

                                                          cGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACAct                                                                            >  2:428642/1‑140 (MQ=255)

                                                                ttGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCaa                                                                                <  2:896174/130‑1 (MQ=255)

                                                                ttGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCaa                                                                                >  1:896174/1‑130 (MQ=255)

                                                                 tGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCgg                                                                     >  2:642668/1‑140 (MQ=255)

                                                                   cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTg                                                                   >  1:171610/1‑140 (MQ=255)

                                                                            aaagacCCGGAAAAACGGGATGGCCCAGGTCGGGCCTTCCTGATGATTATGTGGGGCATTGAG‑G‑GGGGGGCTGATGGGGGCGGGTGTCGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAg                                                          <  2:762062/139‑1 (MQ=255)

                                                                                           ctcgttAGCCCGCTTCGTCCCTTCCTGATGTTTATGTGGGGTATTG‑TGG‑GGGGGGCTGCCCGGGGCGGGTGTCCATTTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAt                                           <  2:23175/135‑1 (MQ=255)

                                                                                           cgcgATGGCCCACGTCGTGCCTTCCTGATAATTATGTGGGGCATTG‑TGG‑GGGGGGCTGATGGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAt                                           <  2:171610/140‑1 (MQ=255)

                                                                                            gcgATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAtt                                          >  1:23175/1‑140 (MQ=255)

                                                                                                tAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGaa                                      <  1:428642/140‑1 (MQ=255)

                                                                                                caGCCCCCGTCGTGCCTCCCTGATGTTTATGTGGGGCTTTG‑TGG‑GGGGGGTTGAGCGGGGCGGGTGTCGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGaa                                      <  2:1095219/139‑1 (MQ=255)

                                                                                                            tGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtgt                          >  1:800591/1‑140 (MQ=255)

                                                                                                            tGCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtgt                          >  1:856679/1‑140 (MQ=255)

                                                                                                             gCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtgtg                         <  1:148097/140‑1 (MQ=255)

                                                                                                             gCCTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtgtg                         <  2:509238/140‑1 (MQ=255)

                                                                                                              ccTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtgctg                        >  1:1095219/1‑137 (MQ=255)

                                                                                                              ccTTCCTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGa                        >  1:719075/1‑140 (MQ=255)

                                                                                                               cttcttGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGaa                       <  1:150150/140‑1 (MQ=255)

                                                                                                                  ccTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGt                    >  1:2910/1‑140 (MQ=255)

                                                                                                                  ccTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGt                    >  1:963665/1‑140 (MQ=255)

                                                                                                                  ccTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGt                    <  1:732345/140‑1 (MQ=255)

                                                                                                                   cTGATGATTATGTGGGGCATTG‑TGG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa                   <  1:105904/140‑1 (MQ=255)

                                                                                                                   cTGATGATTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa                   <  1:477040/140‑1 (MQ=255)

                                                                                                                    tgatgaTTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTac                  >  2:360195/1‑140 (MQ=255)

                                                                                                                     gatgttTATGTGGGGCTTTG‑TGG‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGGTTATTCGAAGAGAATCGATGTGAAAGTact                 <  2:963665/140‑1 (MQ=255)

                                                                                                                      atgttTATGTGGGGCATTG‑TGG‑GGGGGGTTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg                <  2:2910/140‑1 (MQ=255)

                                                                                                                      atgatTATGTGGGGCTTTG‑TGG‑GGGGGGCTGATGGGGGTTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg                <  2:856679/140‑1 (MQ=255)

                                                                                                                      atgatTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg                >  2:830061/1‑140 (MQ=255)

                                                                                                                      atgatTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACGGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg                >  2:94554/1‑140 (MQ=255)

                                                                                                                      atgatTATGTGGGGCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACCGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg                >  2:759298/1‑140 (MQ=255)

                                                                                                                        gtttAGGTGGGGCATTG‑G‑CGGGGGGGCGGATGGGGGCTGGTGTCGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac              <  2:719075/138‑1 (MQ=255)

                                                                                                                        gatTATGTGGGGCATTG‑TGG‑GGGGGGCTGATGGGGGCTGGTGTCGAATTACCCCGCCGTTGCTCGAAACAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac              <  2:800591/140‑1 (MQ=255)

                                                                                                                        gatTATGGGGGGCATTG‑TGG‑GGGGGGCTGATGGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac              <  2:540672/140‑1 (MQ=255)

                                                                                                                          ttATGTGGGGCATTG‑T‑CGGGGGGGCTGATGGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgactg            <  1:642668/140‑1 (MQ=255)

                                                                                                                                  ggCATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATATGAAAGTACTGACTGTATTTGGt    >  1:819475/1‑140 (MQ=255)

                                                                                                                                    cATTG‑T‑CGGGGGGGCTGATCGGGGCTGGTGTCGCATTATCCCGCCGTTGCTCGATATAGCAACTCTGCTGCGGTGTGCGCAAAGGCTCTCGCCGCTTATTCGAAGAGAATCGTTGTGATAGTACTGACTGTATTTGGTAc  >  2:77995/1‑140 (MQ=255)

GGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑T‑C‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTAC  >  NC_000913/3969888‑3970158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A