Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	172	67.8	2301510	98.9%	2276193	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,044,758	T→C	I187T (ATT→ACT)	yihF →	uncharacterized protein YihF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,044,758	0	T	C	100.0%	183.2 / NA	52	I187T (ATT→ACT)	yihF	uncharacterized protein YihF
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/25); total (27/25)

CATTGCGATTCGCGTTTGGTGGCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATATTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGAAATTTAAT  >  NC_000913/4044626‑4044894

cATTGCGATTCGCGTTTGGTGGCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGc                                                                                                                                   >  2:840355/1‑140 (MQ=255)

 aTTGCGATTCGCGTTTGGTGGCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCa                                                                                                                                  >  2:641682/1‑140 (MQ=255)

        ttCGCGTTTGGTGGCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGa                                                                                                                           >  1:1082656/1‑140 (MQ=255)

        ttCGCGTTTGGTGGCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGCGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGa                                                                                                                           <  2:987154/140‑1 (MQ=255)

          cgcgTTTGGTGGCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAg                                                                                                                         >  1:596577/1‑140 (MQ=255)

             gTTTGGTGGCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGata                                                                                                                      <  1:1119603/140‑1 (MQ=255)

             gTTTGGTGGCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGata                                                                                                                      <  1:937808/140‑1 (MQ=255)

                tggtggCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTg                                                                                                                   <  2:998449/140‑1 (MQ=255)

                     gCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTg                                                                                                              <  1:840355/140‑1 (MQ=255)

                     gCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTg                                                                                                              >  2:856187/1‑140 (MQ=255)

                        cATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCaa                                                                                                           <  2:687197/140‑1 (MQ=255)

                         aTTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGa                                                                                                                           <  1:664767/123‑1 (MQ=255)

                         aTTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGa                                                                                                                           >  2:664767/1‑123 (MQ=255)

                              ctACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCa                                                                                                     <  1:999711/140‑1 (MQ=255)

                              ctACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCa                                                                                                     <  1:42639/140‑1 (MQ=255)

                                         gATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACaa                                                                                          >  2:750214/1‑140 (MQ=255)

                                               gCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAAc                                                                                    >  2:342104/1‑140 (MQ=255)

                                                ccccTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACg                                                                                   >  1:663971/1‑140 (MQ=255)

                                                         agTATGGAAAGTTTTCTTTTGGCGAGGGCCAGGTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGt                                                                          >  2:456242/1‑140 (MQ=255)

                                                           tATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGttt                                                                        <  1:641682/140‑1 (MQ=255)

                                                           tATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGttt                                                                        >  2:485921/1‑140 (MQ=255)

                                                            aTGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGtttt                                                                       <  1:360602/140‑1 (MQ=255)

                                                              ggAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTAc                                                                     >  2:854587/1‑140 (MQ=255)

                                                                aaaGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCa                                                                   >  1:290196/1‑140 (MQ=255)

                                                                aaaGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCa                                                                   >  2:82044/1‑140 (MQ=255)

                                                                     tttCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGAt                                                              >  1:850151/1‑140 (MQ=255)

                                                                       tCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGAAGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATtc                                                            >  2:326474/1‑140 (MQ=255)

                                                                        cTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATtct                                                           <  1:139980/140‑1 (MQ=255)

                                                                             ggTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGgc                                                      <  2:660471/140‑1 (MQ=255)

                                                                                  gggCCAGTTTACTTTTAATGGTGAAGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGAt                                                 <  1:326474/140‑1 (MQ=255)

                                                                                   ggCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGAtt                                                >  2:350023/1‑140 (MQ=255)

                                                                                             cTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGaagaaaag                                      >  2:108692/1‑140 (MQ=255)

                                                                                                   aTGGTGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAAttt                                >  2:1019718/1‑140 (MQ=255)

                                                                                                       tGATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCgg                            >  2:831050/1‑140 (MQ=255)

                                                                                                        gATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGt                           >  1:115199/1‑140 (MQ=255)

                                                                                                        gATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGt                           <  1:854587/140‑1 (MQ=255)

                                                                                                        gATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGt                           <  2:850151/140‑1 (MQ=255)

                                                                                                        gATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGt                           <  1:677177/140‑1 (MQ=255)

                                                                                                        gATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGt                           <  1:1110908/140‑1 (MQ=255)

                                                                                                        gATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGt                           <  1:613522/140‑1 (MQ=255)

                                                                                                        gATGGTAGTTCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAACAAATTTCCGGt                           <  2:1080442/140‑1 (MQ=255)

                                                                                                                ttCATTGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGaaa                   >  1:251487/1‑140 (MQ=255)

                                                                                                                     tGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAg              >  2:1140394/1‑140 (MQ=255)

                                                                                                                     tGTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAg              >  2:818423/1‑140 (MQ=255)

                                                                                                                      gTCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGt             >  2:365403/1‑140 (MQ=255)

                                                                                                                       tCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGt                           <  2:449915/125‑1 (MQ=255)

                                                                                                                       tCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGt                           >  1:449915/1‑125 (MQ=255)

                                                                                                                       tCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTc            <  1:1019718/140‑1 (MQ=255)

                                                                                                                       tCTAACCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTc            <  2:1094745/140‑1 (MQ=255)

                                                                                                                           aCCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGaaa        <  1:750214/140‑1 (MQ=255)

                                                                                                                           aCCTGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGaaa        >  1:632479/1‑140 (MQ=255)

                                                                                                                              tGGATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGAAAttt     >  1:207322/1‑140 (MQ=255)

                                                                                                                               ggATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGAAATTTa    <  1:831050/140‑1 (MQ=255)

                                                                                                                                gATACTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGAAATTTaa   <  1:818423/140‑1 (MQ=255)

                                                                                                                                 atacTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGaa         <  1:549662/133‑1 (MQ=255)

                                                                                                                                 atacTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGAAATTTAAt  >  2:769780/1‑140 (MQ=255)

                                                                                                                                 atacTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGAAATTTAAt  <  2:115199/140‑1 (MQ=255)

                                                                                                                                 atacTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGAAATTTAAt  >  1:936206/1‑140 (MQ=255)

CATTGCGATTCGCGTTTGGTGGCTCATTCTCTACGACATTAGATGTTGCCCCTGCAGAGTATGGAAAGTTTTCTTTTGGTGAGGGCCAGTTTACTTTTAATGGTGATGGTAGTTCATTGTCTAACCTGGATATTGAAGGCAAAGTCGAAGATATTGTTCTGCAATTATCACCAATGAACAAAGTAACGGCAAAAAGTTTTACCATTGATTCTCTGGCGCGATTAGAAGAAAAGAAATTTCCGGTTGGTGAAAGCGAGTCGAAATTTAAT  >  NC_000913/4044626‑4044894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A