Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	172	67.8	2301510	98.9%	2276193	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,609,336	(C)_7→8	intergenic (+13/‑78)	yjjG → / → prfC	pyrimidine nucleotidase/peptide chain release factor RF3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,609,329	1	.	C	93.2%	188.1 / 6.5	59	intergenic (+6/‑85)	yjjG/prfC	pyrimidine nucleotidase/peptide chain release factor RF3
Reads supporting (aligned to +/- strand): ref base . (2/2); new base C (30/25); total (32/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

GGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACTTTTGC  >  NC_000913/4609198‑4609463

ggCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCAAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑cc‑cccccc                                                                                                                                >  1:751761/1‑139 (MQ=255)

    tCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑cc‑ccccccgttg                                                                                                                            <  2:979670/140‑6 (MQ=255)

    tCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑cc‑ccccccgttg                                                                                                                            >  2:108005/1‑135 (MQ=255)

     cAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGa                                                                                                                            >  1:448742/1‑140 (MQ=255)

       aCGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATg                                                                                                                          >  1:1053611/1‑140 (MQ=255)

       aCGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATg                                                                                                                          >  2:890964/1‑140 (MQ=255)

        cGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATgg                                                                                                                         <  1:428268/140‑1 (MQ=255)

           cGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTa                                                                                                                      >  2:1034211/1‑140 (MQ=255)

                  gCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGc                                                                                                               >  1:1104254/1‑140 (MQ=255)

                    gACCTGCTGGCTGAATGCACACCATCGCAAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCg                                                                                                             <  2:751761/140‑1 (MQ=255)

                                   tGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTc                                                                                              >  2:874374/1‑140 (MQ=255)

                                    gCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTca                                                                                             >  1:420045/1‑140 (MQ=255)

                                    gCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTca                                                                                             >  1:1031976/1‑140 (MQ=255)

                                       cacCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTcaaca                                                                                          >  1:1041596/1‑140 (MQ=255)

                                           aTCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGttt                                                                                                <  1:1118802/130‑1 (MQ=255)

                                           aTCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGttt                                                                                                >  2:1118802/1‑130 (MQ=255)

                                           aTCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcg                                                                                      <  1:890964/140‑1 (MQ=255)

                                           aTCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑CCACCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcg                                                                                      >  2:506437/1‑140 (MQ=255)

                                              gcgAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcgg                                                                                   >  2:319298/1‑140 (MQ=255)

                                               cgAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcggc                                                                                  >  1:1112602/1‑140 (MQ=255)

                                               cgAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcggc                                                                                  >  1:573634/1‑140 (MQ=255)

                                                    aaCCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGAt                                                                             <  1:319298/140‑1 (MQ=255)

                                                    aaCCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGAt                                                                             <  2:1128392/140‑1 (MQ=255)

                                                         gAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGc                                                                        <  2:1104254/140‑1 (MQ=255)

                                                          aaGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑CCACCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGTGGCGCGATGCCGCt                                                                       <  1:506437/140‑1 (MQ=255)

                                                            ggCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTa                                                                     <  1:108005/140‑1 (MQ=255)

                                                               aTCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTc                                                                  <  2:448742/140‑1 (MQ=255)

                                                               aTCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTAGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTc                                                                  <  1:999533/140‑1 (MQ=255)

                                                                tCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCt                                                                       >  1:1082100/1‑133 (MQ=255)

                                                                tCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCt                                                                       <  2:1082100/133‑1 (MQ=255)

                                                                 cgcgCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaa                                                                <  2:1031976/140‑1 (MQ=255)

                                                                  gcgcCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGc                                                                        <  2:287507/131‑1 (MQ=255)

                                                                  gcgcCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGc                                                                        >  1:287507/1‑131 (MQ=255)

                                                                    gcCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaagaa                                                             >  2:845687/1‑140 (MQ=255)

                                                                           tGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAagaaagaa                                                      <  2:1112602/140‑1 (MQ=255)

                                                                           tGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAagaaagaa                                                      >  1:937884/1‑140 (MQ=255)

                                                                                cGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGCTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATg                                                 <  2:798891/140‑1 (MQ=255)

                                                                                  tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGaagaaag                                                        <  1:370564/131‑1 (MQ=255)

                                                                                  tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGaagaaag                                                        >  2:370564/1‑131 (MQ=255)

                                                                                  tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATg                                                 <  1:422127/138‑1 (MQ=255)

                                                                                  tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATg                                                 >  2:422127/1‑138 (MQ=255)

                                                                                  tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGAc                                               >  2:553861/1‑140 (MQ=255)

                                                                                   ttcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGaagaaa                                                         >  1:1118664/1‑129 (MQ=255)

                                                                                   ttcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg                                              <  1:1102102/140‑1 (MQ=255)

                                                                                   ttcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg                                              <  2:175732/140‑1 (MQ=255)

                                                                                   ttcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATAGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGaagaaa                                                         <  2:1118664/129‑1 (MQ=255)

                                                                                        cGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGt                                          <  1:370730/140‑1 (MQ=255)

                                                                                        cGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGtc                                         <  1:845687/140‑1 (MQ=255)

                                                                                          ttGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGtctc                                       >  1:66104/1‑140 (MQ=255)

                                                                                              aCGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTa                                   <  2:937884/140‑1 (MQ=255)

                                                                                                  aCTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg                                              <  2:347815/125‑1 (MQ=255)

                                                                                                  aCTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg                                              >  1:347815/1‑125 (MQ=255)

                                                                                                    tGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCa                             >  1:959356/1‑140 (MQ=255)

                                                                                                            cTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCa                             >  2:1149135/1‑132 (MQ=255)

                                                                                                            cTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCa                             <  1:1149135/132‑1 (MQ=255)

                                                                                                            cTCCTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTgg                     >  2:794822/1‑140 (MQ=255)

                                                                                                              ccTGTGTAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCg                   >  2:772280/1‑140 (MQ=255)

                                                                                                                 gtgtAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAg                >  2:599751/1‑140 (MQ=255)

                                                                                                                 gtgtAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAg                <  2:66104/140‑1 (MQ=255)

                                                                                                                    tAAACACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAgcgc             >  2:279373/1‑140 (MQ=255)

                                                                                                                      aaCACTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGcgccg           <  2:1041596/140‑1 (MQ=255)

                                                                                                                         acTGATTGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCAc        >  1:1073614/1‑140 (MQ=255)

                                                                                                                               tGCCTC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACTTTTGc  >  2:184231/1‑140 (MQ=255)

GGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACTTTTGC  >  NC_000913/4609198‑4609463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A