Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	182	57.1	1926220	99.1%	1908884	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,865,178	A→G	V26V (GTA→GTG)	ygbN →	putative transporter YgbN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,865,178	0	A	G	100.0%	176.9 / NA	50	V26V (GTA→GTG)	ygbN	putative transporter YgbN
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/30); total (20/30)

TCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCGTCACTATTATTA  >  NC_000913/2865047‑2865305

tCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCAtt                                                                                                                          >  1:111936/1‑140 (MQ=255)

tCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTACACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCAtt                                                                                                                          >  2:917045/1‑140 (MQ=255)

 ccccccATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATGATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTc                                                                                                                         >  2:898808/1‑140 (MQ=255)

  cccccATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCg                                                                                                                        <  2:756684/140‑1 (MQ=255)

   ccccATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGt                                                                                                                       >  1:16214/1‑140 (MQ=255)

   ccccATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGt                                                                                                                       >  1:665949/1‑140 (MQ=255)

   ccccATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGt                                                                                                                       >  2:462424/1‑140 (MQ=255)

      cATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGttgc                                                                                                                    >  1:428323/1‑140 (MQ=255)

      cATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGttgc                                                                                                                    >  1:827012/1‑140 (MQ=255)

              gATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGctcc                                                                                                            <  2:477875/140‑1 (MQ=255)

              gATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCAGTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGctcc                                                                                                            >  1:839657/1‑140 (MQ=255)

               aTGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGctcct                                                                                                           <  1:462424/140‑1 (MQ=255)

                 ggggTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCg                                                                                                         >  2:310403/1‑140 (MQ=255)

                 ggggTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCg                                                                                                         <  1:321548/140‑1 (MQ=255)

                  gggTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTTCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGt                                                                                                        >  2:690259/1‑140 (MQ=255)

                  gggTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGt                                                                                                        >  2:249280/1‑140 (MQ=255)

                  gggTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGt                                                                                                        >  2:104296/1‑140 (MQ=255)

                  gggTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGCGCAACCATTCGTTGCTTTGCTCCTCGt                                                                                                        >  2:110220/1‑140 (MQ=255)

                    gTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCa                                                                                                      <  1:792787/140‑1 (MQ=255)

                    gTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCa                                                                                                      <  2:947843/140‑1 (MQ=255)

                      aGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGc                                                                                                    <  1:898808/140‑1 (MQ=255)

                              tataACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTCCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGt                                                                                            <  2:665949/140‑1 (MQ=255)

                              tataACTTTCAGACAGGGTTTCCCATGTACACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGt                                                                                            <  1:917045/140‑1 (MQ=255)

                                  aCTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCa                                                                                        >  2:4282/1‑140 (MQ=255)

                                      tcagtcagGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTg                                                                                    >  2:686097/1‑140 (MQ=255)

                                       cAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGc                                                                                   <  1:385706/140‑1 (MQ=255)

                                        aGACAGGGTTTCCCATGTTCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCg                                                                                  <  1:690259/140‑1 (MQ=255)

                                          aCAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGc                                                                                <  1:278420/140‑1 (MQ=255)

                                          aCAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGc                                                                                <  1:310403/140‑1 (MQ=255)

                                          aCAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGc                                                                                <  2:839657/140‑1 (MQ=255)

                                                  tCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATAc                                                                        >  1:45541/1‑140 (MQ=255)

                                                            acaATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGa                                                             <  2:16214/140‑1 (MQ=255)

                                                                    aTTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTa                                                     <  2:675729/140‑1 (MQ=255)

                                                                     ttGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGGGGAGgggggga                                                    >  2:824190/1‑137 (MQ=255)

                                                                      tGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTaaa                                                   <  1:686097/140‑1 (MQ=255)

                                                                           tGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGCGGGGGGAAGGGGGGAAAgggg                                               >  2:539871/1‑137 (MQ=255)

                                                                                 gCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGc                                        <  2:428323/140‑1 (MQ=255)

                                                                                  cGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGcc                                       <  1:107933/140‑1 (MQ=255)

                                                                                      aGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGGGGAGGGGGGGAAAAGAAGATTGCCgggg                                   >  2:107933/1‑139 (MQ=255)

                                                                                            cGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCg                             <  1:539871/140‑1 (MQ=255)

                                                                                            cGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCg                             <  1:824190/140‑1 (MQ=255)

                                                                                              tAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGgt                           <  2:646600/140‑1 (MQ=255)

                                                                                              tAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGgt                           <  1:706132/140‑1 (MQ=255)

                                                                                                  cATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGc                       <  2:827012/140‑1 (MQ=255)

                                                                                                   aTGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCt                      <  1:468143/140‑1 (MQ=255)

                                                                                                           gctgctGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCg              <  1:36688/140‑1 (MQ=255)

                                                                                                             tgctgGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCGTc            <  1:4282/140‑1 (MQ=255)

                                                                                                                 ggTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCGTCACta        <  1:884671/140‑1 (MQ=255)

                                                                                                                  gTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCGTCACtat       <  1:826875/140‑1 (MQ=255)

                                                                                                                       caAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCGTCACtattatta  <  1:104296/140‑1 (MQ=255)

TCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCGTCACTATTATTA  >  NC_000913/2865047‑2865305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A