Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	182	57.1	1926220	99.1%	1908884	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,977,063	T→C	C155C (TGT→TGC)	galR →	DNA‑binding transcriptional dual regulator GalR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,977,063	0	T	C	100.0%	196.3 / NA	55	C155C (TGT→TGC)	galR	DNA‑binding transcriptional dual regulator GalR
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/30); total (25/30)

AACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGA  >  NC_000913/2976927‑2977197

aaCTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCAtt                                                                                                                                     >  2:955419/1‑140 (MQ=255)

aaCTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCAtt                                                                                                                                     >  1:857116/1‑140 (MQ=255)

aaCTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCAtt                                                                                                                                     >  2:807442/1‑140 (MQ=255)

 aCTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTg                                                                                                                                    <  1:25266/140‑1 (MQ=255)

 aCTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTg                                                                                                                                    <  2:583144/140‑1 (MQ=255)

 aCTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTg                                                                                                                                    >  1:40740/1‑140 (MQ=255)

  cTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGc                                                                                                                                   <  2:782135/140‑1 (MQ=255)

   tGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGct                                                                                                                                  <  2:245697/140‑1 (MQ=255)

     aTCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGctct                                                                                                                                >  2:364181/1‑140 (MQ=255)

       ccGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTgg                                                                                                                              >  2:229176/1‑140 (MQ=255)

         gctaTCGCTGTGCTGCGTTGGTGGGCGATGCCAAAATGATCCCGGATGTTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCCGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGAc                                                                                                                            <  2:96065/137‑1 (MQ=255)

            aTCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGAt                                                                                                                         >  2:329368/1‑140 (MQ=255)

             tCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATc                                                                                                                        <  2:540916/140‑1 (MQ=255)

                       gcGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTCGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGc                                                                                                              <  2:857116/140‑1 (MQ=255)

                         gTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCct                                                                                                            <  2:40740/140‑1 (MQ=255)

                             gtcgtcCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCctggct                                                                                                        >  1:706232/1‑140 (MQ=255)

                                  ccATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCaa                                                                                                   >  1:861588/1‑140 (MQ=255)

                                  ccATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCaa                                                                                                   >  1:496160/1‑140 (MQ=255)

                                    aTGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAAcg                                                                                                 <  2:379596/140‑1 (MQ=255)

                                      gCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAAcgcg                                                                                               <  2:496160/140‑1 (MQ=255)

                                      gCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAAcgcg                                                                                               <  1:955419/140‑1 (MQ=255)

                                           aaTGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCAtt                                                                                          >  1:746773/1‑140 (MQ=255)

                                             tGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTa                                                                                        <  1:807442/140‑1 (MQ=255)

                                                     gATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCa                                                                                <  2:98728/140‑1 (MQ=255)

                                                            aTTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCAt                                                                         >  1:270933/1‑140 (MQ=255)

                                                             tttAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATa                                                                        <  2:359900/140‑1 (MQ=255)

                                                               tAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATAcc                                                                      <  1:364181/140‑1 (MQ=255)

                                                                 gCCTCATTAATGAAACAAATGCTCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCg                                                                    <  2:706232/140‑1 (MQ=255)

                                                                 gCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCg                                                                    <  1:329368/140‑1 (MQ=255)

                                                                      aTTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTg                                                               >  2:379162/1‑140 (MQ=255)

                                                                        tAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGt                                                             <  2:604792/140‑1 (MQ=255)

                                                                            gAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATc                                                         >  1:455689/1‑140 (MQ=255)

                                                                               aCAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATc                                                         <  2:953392/137‑1 (MQ=255)

                                                                               aCAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATc                                                         >  1:953392/1‑137 (MQ=255)

                                                                                cAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCg                                                                    <  2:906115/125‑1 (MQ=255)

                                                                                cAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCg                                                                    >  1:906115/1‑125 (MQ=255)

                                                                                cAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCtgtg                                                     >  2:327065/1‑140 (MQ=255)

                                                                                cAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCtgtg                                                     >  2:372782/1‑140 (MQ=255)

                                                                                       ccGGTATGGTGCTGATTAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAAc                                              <  2:861588/140‑1 (MQ=255)

                                                                                                   tGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAAc                                              <  2:245429/128‑1 (MQ=255)

                                                                                                   tGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAAc                                              >  1:245429/1‑128 (MQ=255)

                                                                                                    gATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTg                                 <  2:455689/140‑1 (MQ=255)

                                                                                                     aTCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGa                                >  1:213254/1‑140 (MQ=255)

                                                                                                     aTCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGa                                >  1:177451/1‑140 (MQ=255)

                                                                                                               tCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGAt                      >  2:363297/1‑140 (MQ=255)

                                                                                                                    cctgGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCt                 >  1:949534/1‑140 (MQ=255)

                                                                                                                    cctgGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCt                 >  1:754084/1‑140 (MQ=255)

                                                                                                                      tgGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGc               <  2:746773/140‑1 (MQ=255)

                                                                                                                       ggCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCa              >  2:665559/1‑140 (MQ=255)

                                                                                                                        gCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTTAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCaa             <  2:670625/140‑1 (MQ=255)

                                                                                                                        gCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCaa             <  1:612655/140‑1 (MQ=255)

                                                                                                                        gCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCaa             <  1:229176/140‑1 (MQ=255)

                                                                                                                           ttGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAggg          <  1:363297/140‑1 (MQ=255)

                                                                                                                            tGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGt         <  1:327065/140‑1 (MQ=255)

                                                                                                                            tGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGt         <  1:372782/140‑1 (MQ=255)

                                                                                                                                   cGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGa  <  1:665559/140‑1 (MQ=255)

AACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGA  >  NC_000913/2976927‑2977197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A