Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	182	57.1	1926220	99.1%	1908884	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,011,789	T→C	V110A (GTT→GCT)	yqeA →	putative amino acid kinase YqeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,011,789	0	T	C	100.0%	200.3 / NA	59	V110A (GTT→GCT)	yqeA	putative amino acid kinase YqeA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/31); total (28/31)

ACTGAAGCGCACTCGCCAATGCTGCCGATGTCTGTCTGTGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGTTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCTGGTCG  >  NC_000913/3011653‑3011921

aCTGAAGCGCACTCGCCAATGCTGCCGATGTCTGTCTGTGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTAc                                                                                                                                   <  1:153651/140‑1 (MQ=255)

 cTGAAGCGCACTCGCCAATGCTGCCGATGTCTGTCTGTGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACt                                                                                                                                  <  2:397817/140‑1 (MQ=255)

            tCGCCAATGCTGCCGATGTCTGTCTGTGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGt                                                                                                                       <  2:253983/140‑1 (MQ=255)

                      tgcCGATGTCTGTCTGTGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAAc                                                                                                             >  1:320829/1‑140 (MQ=255)

                        ccGATGTCTGTCTGTGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGa                                                                                                           >  1:701572/1‑140 (MQ=255)

                              tctgtctgTGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGc                                                                                                     <  1:237618/140‑1 (MQ=255)

                              tctgtctgTGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGc                                                                                                     <  2:838807/140‑1 (MQ=255)

                                  tctgtGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTc                                                                                                 >  1:201997/1‑140 (MQ=255)

                                  tctgtGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTc                                                                                                 >  1:344570/1‑140 (MQ=255)

                                  tctgtGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTc                                                                                                 >  1:457201/1‑140 (MQ=255)

                                      tgtTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGTTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCa                                                                                             <  1:773991/140‑1 (MQ=255)

                                       gtTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCaa                                                                                            >  1:930443/1‑140 (MQ=255)

                                        ttGCATTAAGCCAGGGTTATATTGGTTACCATCTACAAAGCGGTTTAAGAGAGGACCTGCTTTCTCGGGGCATTAATAAACGCGTAGCAACGCTGGCTACTCAGGTGGAAGTCGACGCTGACGATCCGGCATTCCTCAAc                                                                                           <  2:344570/140‑1 (MQ=255)

                                         tGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAAcc                                                                                          >  2:809260/1‑140 (MQ=255)

                                           cATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCg                                                                                        <  2:559578/140‑1 (MQ=255)

                                            aTTAAGCCAGGGTTATATTGGTTACGATCTACAAACCGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGa                                                                                       >  1:743104/1‑140 (MQ=255)

                                            aTTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGa                                                                                       <  2:457201/140‑1 (MQ=255)

                                            aTTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGa                                                                                       <  2:201997/140‑1 (MQ=255)

                                             ttAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGc                                                                                                     <  2:239842/125‑1 (MQ=255)

                                             ttAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGc                                                                                                     >  1:239842/1‑125 (MQ=255)

                                              tAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGAcc                                                                                     <  2:393317/140‑1 (MQ=255)

                                                aGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACGTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCaa                                                                                   >  2:834735/1‑140 (MQ=255)

                                                aGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCaa                                                                                            <  2:14215/131‑1 (MQ=255)

                                                aGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCaa                                                                                            >  1:14215/1‑131 (MQ=255)

                                                aGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCaa                                                                                   <  2:701572/140‑1 (MQ=255)

                                                      ggTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGAt                                                                             >  2:515522/1‑140 (MQ=255)

                                                          atatTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGgt                                                                         <  1:475788/140‑1 (MQ=255)

                                                          atatTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGgt                                                                         <  1:32877/140‑1 (MQ=255)

                                                           tatTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGgtt                                                                        >  1:377280/1‑140 (MQ=255)

                                                               ggTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGgttcgtt                                                                    <  2:729024/140‑1 (MQ=255)

                                                                gTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGgttcgttc                                                                   >  1:304581/1‑140 (MQ=255)

                                                                  tACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCtt                                                                 <  2:320829/140‑1 (MQ=255)

                                                                           cAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCa                                                        <  1:809260/140‑1 (MQ=255)

                                                                                   tttAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCag                                                >  2:953315/1‑140 (MQ=255)

                                                                                          aaagaaCTGCTTTCTCGGGGCATTAATAACCCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACt                                         <  2:823838/139‑1 (MQ=255)

                                                                                            agaaCTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCagag                                              >  1:850411/1‑133 (MQ=255)

                                                                                            agaaCTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCagag                                              <  2:850411/133‑1 (MQ=255)

                                                                                            agaaCTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGa                                       <  1:953315/140‑1 (MQ=255)

                                                                                             gaaCTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGAc                                      >  1:455229/1‑140 (MQ=255)

                                                                                             gaaCTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCT‑AACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGa                                       >  2:466831/1‑138 (MQ=255)

                                                                                             gaaCTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCT‑AACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGa                                       <  1:466831/138‑1 (MQ=255)

                                                                                                 tGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACaaaa                                  <  2:749352/140‑1 (MQ=255)

                                                                                                 tGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACaaaa                                  <  1:515522/140‑1 (MQ=255)

                                                                                                   cTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACa                                >  1:834546/1‑140 (MQ=255)

                                                                                                   cTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACa                                <  1:563847/140‑1 (MQ=255)

                                                                                                     ttCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAgg                              >  2:190629/1‑140 (MQ=255)

                                                                                                         cGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTa                          <  1:458833/140‑1 (MQ=255)

                                                                                                           gggCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGTGCAAGAAGCAGAGCAACTGACAAAACAGGGTTaca                        <  1:953962/140‑1 (MQ=255)

                                                                                                           gggCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTaca                        <  2:455229/140‑1 (MQ=255)

                                                                                                             gCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACAct                      <  1:672815/140‑1 (MQ=255)

                                                                                                                      aaCCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATTGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAGCTGACGACACAGGGCTACACTCTGAaagaa             >  2:672815/1‑140 (MQ=255)

                                                                                                                           gTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGc        >  1:642894/1‑140 (MQ=255)

                                                                                                                           gTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACAATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAaag               <  1:257296/133‑1 (MQ=255)

                                                                                                                           gTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACAATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAaag               >  2:257296/1‑133 (MQ=255)

                                                                                                                            tAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCt       >  2:608376/1‑140 (MQ=255)

                                                                                                                            tAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCt       >  2:469004/1‑140 (MQ=255)

                                                                                                                            tAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCt       >  1:72538/1‑140 (MQ=255)

                                                                                                                             aGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCTg      >  1:111632/1‑140 (MQ=255)

                                                                                                                             aGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATAGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCTg      >  2:889478/1‑140 (MQ=255)

                                                                                                                                 aCGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCTGGTcg  <  2:930443/140‑1 (MQ=255)

ACTGAAGCGCACTCGCCAATGCTGCCGATGTCTGTCTGTGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGTTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCTGGTCG  >  NC_000913/3011653‑3011921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A