Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	182	57.1	1926220	99.1%	1908884	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,267,951	T→C	V33A (GTA→GCA)	yhaB →	protein YhaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,267,951	0	T	C	100.0%	216.2 / NA	60	V33A (GTA→GCA)	yhaB	protein YhaB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (34/26); total (34/26)

TTATTGTTTGTCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATG  >  NC_000913/3267821‑3268084

ttattGTTTGTCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAaaagaaa                                                                                                                              >  2:7363/1‑140 (MQ=255)

      tttgtCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGc                                                                                                                        >  1:539311/1‑140 (MQ=255)

       ttgtCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCa                                                                                                                       >  1:950200/1‑140 (MQ=255)

        tgtCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAg                                                                                                                      >  1:619337/1‑140 (MQ=255)

        tgtCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAg                                                                                                                      >  2:71370/1‑140 (MQ=255)

        tgtCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAg                                                                                                                      >  2:368062/1‑140 (MQ=255)

        tgtCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAg                                                                                                                      >  1:355546/1‑140 (MQ=255)

          tCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGaa                                                                                                                    >  2:746874/1‑140 (MQ=255)

          tCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGaa                                                                                                                    >  1:430933/1‑140 (MQ=255)

           cATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGaaa                                                                                                                   <  1:71370/140‑1 (MQ=255)

           cATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGaaa                                                                                                                   <  1:502767/140‑1 (MQ=255)

                tgaCAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCCCCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGtc                                                                                                              <  2:208156/140‑1 (MQ=255)

                tgaCAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGtc                                                                                                              <  1:385887/140‑1 (MQ=255)

                tgaCAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGtc                                                                                                              >  2:385887/1‑140 (MQ=255)

                  aCAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGtctc                                                                                                            <  2:918230/140‑1 (MQ=255)

                   cAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTcc                                                                                                           <  1:368062/140‑1 (MQ=255)

                    aaGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTcca                                                                                                          >  1:709249/1‑140 (MQ=255)

                         aggcaTCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGtctc                                                                                                            >  1:748244/1‑133 (MQ=255)

                         aggcaTCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGtctc                                                                                                            <  2:748244/133‑1 (MQ=255)

                         aggcaTCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAAt                                                                                                     <  2:6219/140‑1 (MQ=255)

                                  tggaaGGCTTTCCCAATGGGCATATTTTTCACCCTTTAATTCCGGCCAAGGC‑CGCAGATGGTAACAAACACAATAGAAATATTGATTATTTGGCGGCCAAAAGAAAGGTTGCAGAAAATGTCTCCACCAATGACGTtaat                                                                                            >  2:666714/4‑140 (MQ=255)

                                     aaGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATaaa                                                                                         >  2:462292/1‑140 (MQ=255)

                                     aaGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATaaa                                                                                         <  1:462292/140‑1 (MQ=255)

                                      aGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATaaaa                                                                                        >  2:519455/1‑140 (MQ=255)

                                         cTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAAttt                                                                                     <  2:234386/140‑1 (MQ=255)

                                         cTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGACTCCACCAATGACGTTAATAAAAttt                                                                                     <  1:389560/140‑1 (MQ=255)

                                          ttCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTa                                                                                    >  1:217372/1‑140 (MQ=255)

                                            cccAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACa                                                                                  >  2:459162/1‑140 (MQ=255)

                                              cAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGt                                                                                <  1:666714/140‑1 (MQ=255)

                                                aTTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGtat                                                                              <  1:746874/140‑1 (MQ=255)

                                                    cgcATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGt                                                                          >  1:775535/1‑140 (MQ=255)

                                                    cgcATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGt                                                                          >  2:611807/1‑140 (MQ=255)

                                                     gcATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTa                                                                         <  1:611807/140‑1 (MQ=255)

                                                      cATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTaa                                                                        >  1:641777/1‑140 (MQ=255)

                                                          tttttCCCCCTTAAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGaa                                                                    <  2:298895/140‑1 (MQ=255)

                                                            tttCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACt                                                                  <  2:539311/140‑1 (MQ=255)

                                                               cACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCg                                                               <  2:355546/140‑1 (MQ=255)

                                                               cACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCg                                                               <  2:266189/140‑1 (MQ=255)

                                                                aCCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCgg                                                              <  2:709249/140‑1 (MQ=255)

                                                                aCCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCgg                                                              <  1:519455/140‑1 (MQ=255)

                                                                aCCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCgg                                                              <  2:775535/140‑1 (MQ=255)

                                                                 cccTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGga                                                             <  2:217372/140‑1 (MQ=255)

                                                                             cGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCt                                                  >  1:553417/1‑140 (MQ=255)

                                                                               ccAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGc                                                >  2:951711/1‑140 (MQ=255)

                                                                                           gTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCAtt                                    <  2:641777/140‑1 (MQ=255)

                                                                                             ggTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGTAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCTGAGAGTATTATCTGCAATAACTGCATTGa                                  >  1:386873/1‑140 (MQ=255)

                                                                                               ttAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAAt                                >  1:906969/1‑140 (MQ=255)

                                                                                                      tCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATc                         >  1:166627/1‑140 (MQ=255)

                                                                                                      tCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATc                         >  1:932512/1‑140 (MQ=255)

                                                                                                      tCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATc                         >  1:330574/1‑140 (MQ=255)

                                                                                                      tCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATc                         >  2:364133/1‑140 (MQ=255)

                                                                                                        acaATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCAt                       >  1:939697/1‑140 (MQ=255)

                                                                                                        acaATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCAt                       >  1:747855/1‑140 (MQ=255)

                                                                                                        acaATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCAt                       >  2:372278/1‑140 (MQ=255)

                                                                                                         caATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATg                      <  2:330574/140‑1 (MQ=255)

                                                                                                                     tGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCtt          <  2:747855/140‑1 (MQ=255)

                                                                                                                      gATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTc         >  2:479287/1‑140 (MQ=255)

                                                                                                                         tattGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTAAAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGt      >  2:372738/1‑140 (MQ=255)

                                                                                                                            tGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCAt   <  1:372278/140‑1 (MQ=255)

                                                                                                                            tGGACGGCAAAAAGAAAGTTTGCAGAAATCGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCAt   >  2:137828/1‑140 (MQ=255)

                                                                                                                             ggACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCAtt  >  2:279544/1‑139 (MQ=255)

TTATTGTTTGTCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATG  >  NC_000913/3267821‑3268084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A