Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	182	57.1	1926220	99.1%	1908884	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,284,795	T→C	C37C (TGT→TGC)	agaC →	galactosamine‑specific PTS enzyme IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,284,795	0	T	C	100.0%	162.7 / NA	46	C37C (TGT→TGC)	agaC	galactosamine‑specific PTS enzyme IIC component
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/22); total (24/22)

CCTTAATATTGGTTTGAGGTAATAAAAATGCATGAAATAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGTACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCA  >  NC_000913/3284658‑3284931

ccTTAATATTGGTTTGAGGTAATAAAAATGCATGAAATAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCAc                                                                                                                                        >  1:56250/1‑140 (MQ=255)

           gTTTGAGGTAATAAAAATGCATGAAATAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTGTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGgcg                                                                                                                             <  2:955366/140‑1 (MQ=255)

           gTTTGAGGTAATAAAAATGCATGAAATAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGgcg                                                                                                                             >  1:516487/1‑140 (MQ=255)

               gAGGTAATAAAAATGCATGAAATAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTAt                                                                                                                         >  1:951826/1‑140 (MQ=255)

                  gTAATAAAAATGCATGAAATAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATtct                                                                                                                      <  2:238360/140‑1 (MQ=255)

                      taaAAATGCATGAAATAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGt                                                                                                                  <  1:608093/140‑1 (MQ=255)

                       aaaaaTGCATGAAATAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTg                                                                                                                 <  1:705887/140‑1 (MQ=255)

                                tGAAATAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGa                                                                                                        <  2:910630/140‑1 (MQ=255)

                                   aaTAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTg                                                                                                     <  2:56250/140‑1 (MQ=255)

                                     tAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGc                                                                                                   <  2:516487/140‑1 (MQ=255)

                                      aaCCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCt                                                                                                  <  2:168964/140‑1 (MQ=255)

                                        cccTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTa                                                                                                >  2:67471/1‑140 (MQ=255)

                                                cAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCgg                                                                                        <  2:757838/140‑1 (MQ=255)

                                                  gggATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCggtg                                                                                      >  2:66748/1‑140 (MQ=255)

                                                  gggATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCggtg                                                                                      >  1:800880/1‑140 (MQ=255)

                                                   ggATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCggtgg                                                                                     >  1:272983/1‑140 (MQ=255)

                                                   ggATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACGGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCggtgg                                                                                     >  2:634459/1‑140 (MQ=255)

                                                        aTCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGa                                                                                <  1:947458/140‑1 (MQ=255)

                                                        aTCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGa                                                                                >  2:111127/1‑140 (MQ=255)

                                                        aTCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGa                                                                                >  1:609510/1‑140 (MQ=255)

                                                        aTCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGa                                                                                >  1:287043/1‑140 (MQ=255)

                                                        aTCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCACTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGa                                                                                >  1:739477/1‑140 (MQ=255)

                                                             tGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACagag                                                                           >  1:709269/1‑140 (MQ=255)

                                                             tGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACagag                                                                           <  2:287043/140‑1 (MQ=255)

                                                               gcggcgTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGtt                                                                         >  1:137206/1‑140 (MQ=255)

                                                                     ttAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCttt                                                                   >  1:208352/1‑140 (MQ=255)

                                                                       aGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCg                                                                 <  1:67471/140‑1 (MQ=255)

                                                                        gTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGc                                                                <  2:153452/140‑1 (MQ=255)

                                                                         tttttGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGcc                                                               <  2:636724/140‑1 (MQ=255)

                                                                             tGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCg                                                                 <  2:620820/134‑1 (MQ=255)

                                                                             tGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCg                                                                 >  1:620820/1‑134 (MQ=255)

                                                                               ttCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGcc                                                               <  1:750730/134‑1 (MQ=255)

                                                                               ttCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGcc                                                               >  2:750730/1‑134 (MQ=255)

                                                                               ttCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTa                                                         >  1:189072/1‑140 (MQ=255)

                                                                                 cTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCACTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAAc                                                       <  2:739477/140‑1 (MQ=255)

                                                                                  tGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAAcc                                                      >  1:76311/1‑140 (MQ=255)

                                                                                   ggggATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAAccc                                                     >  1:572441/1‑140 (MQ=255)

                                                                                   ggggATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAAccc                                                     >  2:417767/1‑140 (MQ=255)

                                                                                          gattTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAgg                                              <  1:560857/140‑1 (MQ=255)

                                                                                          gattTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAgg                                              <  2:272983/140‑1 (MQ=255)

                                                                                                                  ttATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGAt                      <  2:467774/140‑1 (MQ=255)

                                                                                                                         gCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCg               >  1:877185/1‑140 (MQ=255)

                                                                                                                          cccGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCgg              >  2:146257/1‑140 (MQ=255)

                                                                                                                          cccGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCgg              >  2:139161/1‑140 (MQ=255)

                                                                                                                          cccGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCgg              <  1:139161/140‑1 (MQ=255)

                                                                                                                               tAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCtg         <  1:111127/140‑1 (MQ=255)

                                                                                                                                      ttGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGacca  <  1:66748/140‑1 (MQ=255)

CCTTAATATTGGTTTGAGGTAATAAAAATGCATGAAATAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGTACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCA  >  NC_000913/3284658‑3284931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A