Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	182	57.1	1926220	99.1%	1908884	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,526,366	T→C	intergenic (‑217/‑103)	nudE ← / → igaA	ADP‑sugar diphosphatase NudE/inner membrane protein ‑ inhibits the Rcs signaling pathway

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,526,366	0	T	C	100.0%	207.9 / NA	59	intergenic (‑217/‑103)	nudE/igaA	ADP‑sugar diphosphatase NudE/inner membrane protein ‑ inhibits the Rcs signaling pathway
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/31); total (28/31)

ACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATGAGCACCATTGTGATTTTTTTAGCTGCTTTG  >  NC_000913/3526229‑3526501

aCATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGc                                                                                                                                       >  2:449518/1‑140 (MQ=255)

    ttATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCaa                                                                                                                                   >  2:793370/1‑140 (MQ=255)

         tAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTaa                                                                                                                              >  2:236849/1‑140 (MQ=255)

           aCTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAAc                                                                                                                            >  2:155275/1‑140 (MQ=255)

            cTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTc                                                                                                                                     <  2:87461/130‑1 (MQ=255)

            cTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTc                                                                                                                                     >  1:87461/1‑130 (MQ=255)

            cTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACt                                                                                                                           >  2:894056/1‑140 (MQ=255)

             ttAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTc                                                                                                                          <  2:222588/140‑1 (MQ=255)

              taataaATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTcc                                                                                                                         >  1:559323/1‑140 (MQ=255)

               aataaataATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGCAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTa                                                                                                                               <  2:860747/133‑1 (MQ=255)

               aataaataATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTa                                                                                                                               >  1:860747/1‑133 (MQ=255)

                       atCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAATTCCACGCTTGcc                                                                                                                >  2:606900/1‑140 (MQ=255)

                       atCGTCCCCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGcc                                                                                                                >  1:114993/1‑140 (MQ=255)

                           tCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCa                                                                                                                        <  1:872544/128‑1 (MQ=255)

                           tCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCa                                                                                                                        >  2:872544/1‑128 (MQ=255)

                              tcaaatcaaaTTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGcc                                                                                                         <  1:236849/140‑1 (MQ=255)

                              tcaaatcaaaTTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGcc                                                                                                         <  1:449518/140‑1 (MQ=255)

                              tcaaatcaaaTTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGcc                                                                                                         <  1:155275/140‑1 (MQ=255)

                               caaatcaaatTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCa                                                                                                                        >  2:948507/1‑124 (MQ=255)

                               caaatcaaatTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCa                                                                                                                        <  1:948507/124‑1 (MQ=255)

                               caaatcaaatTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCa                                                                                                        >  1:111961/1‑140 (MQ=255)

                               caaatcaaatTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCa                                                                                                        >  1:170996/1‑140 (MQ=255)

                                 aatcaaatTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAAc                                                                                                      >  1:330954/1‑140 (MQ=255)

                                       atTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCTGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGa                                                                                                >  2:517215/1‑140 (MQ=255)

                                       atTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGa                                                                                                <  2:111961/140‑1 (MQ=255)

                                       atTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGa                                                                                                <  2:114993/140‑1 (MQ=255)

                                       atTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGa                                                                                                <  2:482797/140‑1 (MQ=255)

                                       atTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGa                                                                                                <  2:330954/140‑1 (MQ=255)

                                            aaGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAATTCCACGCTTGCCGATAGCCAACCGCAGAATCAt                                                                                           <  1:606900/140‑1 (MQ=255)

                                                 aaTAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTAtt                                                                                      <  1:793370/140‑1 (MQ=255)

                                                 aaTAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTAtt                                                                                      >  2:749237/1‑140 (MQ=255)

                                                         gAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCgg                                                                              >  1:504721/1‑140 (MQ=255)

                                                         gAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCgg                                                                              <  2:351127/140‑1 (MQ=255)

                                                              aaCTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGa                                                                         <  2:618036/140‑1 (MQ=255)

                                                                 tCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTg                                                                      >  1:522975/1‑140 (MQ=255)

                                                                  ccAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGa                                                                     <  1:894056/140‑1 (MQ=255)

                                                                   cAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGAc                                                                    <  2:579632/140‑1 (MQ=255)

                                                                     ggAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGAc                                                                    >  1:689958/1‑138 (MQ=255)

                                                                     ggAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGAc                                                                    <  2:689958/138‑1 (MQ=255)

                                                                         tttGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGcc                                                              >  2:382459/1‑140 (MQ=255)

                                                                           tGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTg                                                            <  1:601078/140‑1 (MQ=255)

                                                                           tGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTg                                                            >  1:379411/1‑140 (MQ=255)

                                                                            gCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGa                                                           <  2:600430/140‑1 (MQ=255)

                                                                            gCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGa                                                           <  1:517215/140‑1 (MQ=255)

                                                                            gCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGa                                                           <  2:823421/140‑1 (MQ=255)

                                                                            gCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGa                                                           <  2:116909/140‑1 (MQ=255)

                                                                            gCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGa                                                           <  2:504721/140‑1 (MQ=255)

                                                                              tGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACa                                                         <  2:170996/140‑1 (MQ=255)

                                                                                     ccGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACtaag                                                  >  2:167829/1‑140 (MQ=255)

                                                                                          ttCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGa                                             <  2:531706/140‑1 (MQ=255)

                                                                                          ttCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGa                                             <  1:382459/140‑1 (MQ=255)

                                                                                                      gCTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCAt                                 >  2:930333/1‑140 (MQ=255)

                                                                                                       cTATGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATg                                >  1:265251/1‑140 (MQ=255)

                                                                                                         aTGATCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATGAg                              <  1:930333/140‑1 (MQ=255)

                                                                                                            aTCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATGAg                              <  2:25155/137‑1 (MQ=255)

                                                                                                            aTCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATGAg                              >  1:25155/1‑137 (MQ=255)

                                                                                                            aTCAGCGGTTACTGTGATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATGAGCAc                           >  1:85640/1‑140 (MQ=255)

                                                                                                                          tgATGTGCTTAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATGAGCACCATTGTGAtttttt             <  2:430271/140‑1 (MQ=255)

                                                                                                                                  ttAATGACGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATGAGCACCATTGTGATTTTTTTAgctgct     >  1:30982/1‑140 (MQ=255)

                                                                                                                                     atgacgCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATGAGCACCATTGTGATTTTTTTAGCTGCTTTg  <  2:265251/140‑1 (MQ=255)

ACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATGAGCACCATTGTGATTTTTTTAGCTGCTTTG  >  NC_000913/3526229‑3526501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A