Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	182	57.1	1926220	99.1%	1908884	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,948,805	(G)_6→7	coding (1165/1521 nt)	yifB ←	putative magnesium chelatase YifB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,948,799	1	.	G	100.0%	156.4 / NA	45	G391R (GGC→CGC)	yifB	putative magnesium chelatase YifB
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (23/22); total (23/22)

GAATTGGCGTATTTCCGGACTATCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGC  >  NC_000913/3948662‑3948930

gAATTGGCGTATTTCCGGACTATCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑gg                                                                                                                                   <  1:41619/140‑1 (MQ=255)

    tGGCGTATTTCCGGACTATCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑gggggg                                                                                                                               <  2:582510/140‑1 (MQ=255)

    tGGCGTATTTCCGGACTATCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑gggggg                                                                                                                               <  1:45973/140‑1 (MQ=255)

    tGGCGTATTTCCGGACTATCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑gggggg                                                                                                                               <  1:679019/140‑1 (MQ=255)

     ggCGTATTTCCGGACTATCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑ggggggg                                                                                                                              <  2:565048/140‑2 (MQ=255)

      gCGTATTTCCGGACTATCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTGGCGCCCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑gggggggt                                                                                                                             >  1:539410/1‑138 (MQ=255)

           tttCCGGACTATCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑gggggg                                                                                                                               <  2:845436/133‑1 (MQ=255)

           tttCCGGACTATCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑gggggg                                                                                                                               >  1:845436/1‑133 (MQ=255)

            ttCCGGACTATCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAAt                                                                                                                        <  1:166248/140‑1 (MQ=255)

                 gACTATCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTGGCGCCCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGAt                                                                                                                   <  2:539410/140‑1 (MQ=255)

                    tATCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGAtctc                                                                                                                <  2:704834/140‑1 (MQ=255)

                      tCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCa                                                                                                              >  1:93991/1‑140 (MQ=255)

                      tCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCa                                                                                                              >  1:840649/1‑140 (MQ=255)

                      tCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCa                                                                                                              >  1:741288/1‑140 (MQ=255)

                      tCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑ggggggg                                                                                                                              <  2:902336/123‑2 (MQ=255)

                      tCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑ggggggg                                                                                                                              >  1:902336/1‑122 (MQ=255)

                           ccagGCATTCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTga                                                                                                          >  2:825863/1‑139 (MQ=255)

                           ccagGCATTCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTga                                                                                                          <  1:825863/139‑1 (MQ=255)

                              ggCATTCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGCGGAAATGGGATCTCCAGTgagaga                                                                                                      >  1:114831/1‑140 (MQ=255)

                                cATTCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATc                                                                                                    >  2:809048/1‑140 (MQ=255)

                                   tCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGaa                                                                                                 <  2:741288/140‑1 (MQ=255)

                                    cAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAg                                                                                                >  2:832605/1‑140 (MQ=255)

                                        ttATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGt                                                                                            <  1:860929/140‑1 (MQ=255)

                                         tATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTc                                                                                           >  1:672921/1‑140 (MQ=255)

                                         tATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTc                                                                                           >  1:467829/1‑140 (MQ=255)

                                               gCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGaaa                                                                                     <  2:672921/140‑1 (MQ=255)

                                               gCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGaaa                                                                                     >  1:301203/1‑140 (MQ=255)

                                               gCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGaaa                                                                                     >  2:257869/1‑140 (MQ=255)

                                                ccGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAg                                                                                    <  2:467829/140‑1 (MQ=255)

                                                           ggCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGagag                                                                         <  1:173034/140‑1 (MQ=255)

                                                                tctctGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGt                                                                    <  1:832605/140‑1 (MQ=255)

                                                                  tctGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTg                                                                  >  1:415893/1‑140 (MQ=255)

                                                                      gcggcCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGAt                                                              <  1:76520/140‑1 (MQ=255)

                                                                      gcggcCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGAt                                                              <  2:415893/140‑1 (MQ=255)

                                                                            aTTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAGGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTa                                                        <  1:171126/140‑1 (MQ=255)

                                                                            aTTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAGGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTa                                                        >  2:171126/1‑140 (MQ=255)

                                                                            aTTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTa                                                        >  2:235809/1‑140 (MQ=255)

                                                                            aTTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACATa                                                        >  2:364246/1‑140 (MQ=255)

                                                                              tatgcgTTGTTTAACGGTGGCGCTGCTTTCACCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAAt                                                      <  2:589941/137‑1 (MQ=255)

                                                                                      gttTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTc                                              >  2:406055/1‑140 (MQ=255)

                                                                                      gttTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTc                                              >  2:201238/1‑140 (MQ=255)

                                                                                               tGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCa                                     >  1:348050/1‑140 (MQ=255)

                                                                                                 gcgcTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGc                                   >  2:77679/1‑140 (MQ=255)

                                                                                                   gctgctTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCgg                                 >  1:905998/1‑140 (MQ=255)

                                                                                                   gctgctTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGCCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCgg                                 >  1:678731/1‑140 (MQ=255)

                                                                                                       ctTTCTCCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTAt                             >  1:563709/1‑140 (MQ=255)

                                                                                                          tctcCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGt                          <  1:235809/140‑1 (MQ=255)

                                                                                                          tctcCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGt                          <  1:257869/140‑1 (MQ=255)

                                                                                                          tctcCCGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGt                          <  2:301203/140‑1 (MQ=255)

                                                                                                              ccGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTcc                      <  2:840649/140‑1 (MQ=255)

                                                                                                              ccGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTcc                      <  1:835814/140‑1 (MQ=255)

                                                                                                                  cACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGa                  <  1:809048/140‑1 (MQ=255)

                                                                                                                      aCCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAAt              <  1:831686/140‑1 (MQ=255)

                                                                                                                               cTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAg     >  2:868634/1‑140 (MQ=255)

                                                                                                                                  aaaaTGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGc  <  1:868634/140‑1 (MQ=255)

GAATTGGCGTATTTCCGGACTATCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTGGCGCTCTCTGGCGGCCATTACGCGTTGTTTAACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTCGAGAAAGGGCCCCGAGAGCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGC  >  NC_000913/3948662‑3948930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A