Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	182	57.1	1926220	99.1%	1908884	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,028,640	G→A	L45L (CTG→TTG)	fadA ←	3‑ketoacyl‑CoA thiolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,028,640	0	G	A	100.0%	229.7 / NA	65	L45L (CTG→TTG)	fadA	3‑ketoacyl‑CoA thiolase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (34/31); total (34/31)

GCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTG  >  NC_000913/4028506‑4028776

gCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCg                                                                                                                                     >  1:312484/1‑140 (MQ=255)

 cGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCgg                                                                                                                                    >  1:741794/1‑140 (MQ=255)

   aTTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGt                                                                                                                                  >  2:291123/1‑140 (MQ=255)

             aCCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCcag                                                                                                                        >  2:870454/1‑140 (MQ=255)

             aCCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCcag                                                                                                                        <  1:877892/140‑1 (MQ=255)

                  cgGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGc                                                                                                                   <  2:312484/140‑1 (MQ=255)

                    ggACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACCCACCCCCAGTAATTATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTa                                                                                                                 <  2:122844/140‑1 (MQ=255)

                    ggACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTa                                                                                                                 <  2:741794/140‑1 (MQ=255)

                         gagTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCAt                                                                                                            >  1:419227/1‑140 (MQ=255)

                            tgtgGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTaa                                                                                                         >  1:733797/1‑140 (MQ=255)

                            tgtgGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTaa                                                                                                         >  2:313342/1‑140 (MQ=255)

                             gtgGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTaaa                                                                                                        <  1:725790/140‑1 (MQ=255)

                               ggTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATg                                                                                                      >  2:778089/1‑140 (MQ=255)

                                 tACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAg                                                                                                    <  1:870454/140‑1 (MQ=255)

                                   cTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCg                                                                                                  <  1:291123/140‑1 (MQ=255)

                                      cTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGgag                                                                                               >  1:46698/1‑140 (MQ=255)

                                      cTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGgag                                                                                               >  2:578347/1‑140 (MQ=255)

                                           agcagcGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAg                                                                                                    >  2:229927/1‑130 (MQ=255)

                                           agcagcGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAg                                                                                                    <  1:229927/130‑1 (MQ=255)

                                            gcagcGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAtct                                                                                         <  2:791650/140‑1 (MQ=255)

                                             cagcGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGa                                                                                                     <  1:403952/127‑1 (MQ=255)

                                             cagcGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGa                                                                                                     >  2:403952/1‑127 (MQ=255)

                                             cagcGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAtctt                                                                                        >  1:961899/1‑140 (MQ=255)

                                                    gcgTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacg                                                                                 <  2:290767/140‑1 (MQ=255)

                                                               gATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGaa                                                                      <  2:419227/140‑1 (MQ=255)

                                                               gATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGaa                                                                      <  2:602961/140‑1 (MQ=255)

                                                               gATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGaa                                                                      <  1:313342/140‑1 (MQ=255)

                                                               gATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGaa                                                                      <  1:719273/140‑1 (MQ=255)

                                                                  atTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAg                                                                   <  1:455599/140‑1 (MQ=255)

                                                                         cccTGCTCCAGCGTCTGCTGCACACATCCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGcc                                                            <  2:47716/140‑1 (MQ=255)

                                                                           cTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCt                                                          >  2:807737/1‑140 (MQ=255)

                                                                           cTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCt                                                          <  2:733797/140‑1 (MQ=255)

                                                                            tGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCtt                                                         >  2:761570/1‑140 (MQ=255)

                                                                            tGCTACAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCtt                                                         >  2:279297/1‑140 (MQ=255)

                                                                             gCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTc                                                        >  1:304417/1‑140 (MQ=255)

                                                                             gCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTc                                                        >  1:885130/1‑140 (MQ=255)

                                                                              cTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCg                                                       >  1:716633/1‑140 (MQ=255)

                                                                              cTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCg                                                       >  2:645305/1‑140 (MQ=255)

                                                                                        tgctgcACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCAt                                             >  2:549267/1‑140 (MQ=255)

                                                                                              acacaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGt                                       <  1:778089/140‑1 (MQ=255)

                                                                                              acacaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGt                                       <  2:716633/140‑1 (MQ=255)

                                                                                                    ccccAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCGTCGGGGTGCGAAt                                 >  2:747149/1‑140 (MQ=255)

                                                                                                    ccccAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAAt                                 >  1:737496/1‑140 (MQ=255)

                                                                                                       cAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCggg                                         >  2:343539/1‑129 (MQ=255)

                                                                                                       cAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCggg                                         <  1:343539/129‑1 (MQ=255)

                                                                                                       cAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGc                              >  1:278782/1‑140 (MQ=255)

                                                                                                       cAGTAAATATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGc                              >  2:143235/1‑140 (MQ=255)

                                                                                                            aaTATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGTACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGa                         <  1:527713/140‑1 (MQ=255)

                                                                                                            aaTATCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGa                         <  1:735001/140‑1 (MQ=255)

                                                                                                              tatCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCa                             <  1:35335/134‑1 (MQ=255)

                                                                                                              tatCGTCGAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCa                             >  2:35335/1‑134 (MQ=255)

                                                                                                                 cgtcgAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATg                    >  1:434662/1‑140 (MQ=255)

                                                                                                                 cgtccAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATg                    <  2:885130/140‑1 (MQ=255)

                                                                                                                  gtcgAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGa                   >  1:153368/1‑140 (MQ=255)

                                                                                                                   tcgAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGAc                  >  1:96410/1‑140 (MQ=255)

                                                                                                                    cgAGGGCCGCCGCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACa                 <  1:645305/140‑1 (MQ=255)

                                                                                                                          ccgccgCTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCGTCGGGGTGCGAATTGCATCGACAATGACAACCTGt           <  1:747149/140‑1 (MQ=255)

                                                                                                                           cgccgcTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGtt          >  1:236661/1‑140 (MQ=255)

                                                                                                                            gccgcTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTc         <  1:143235/140‑1 (MQ=255)

                                                                                                                             ccgcTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATg                    <  2:606512/128‑1 (MQ=255)

                                                                                                                             ccgcTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATg                    >  1:606512/1‑128 (MQ=255)

                                                                                                                             ccgcTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTcc        >  2:352333/1‑140 (MQ=255)

                                                                                                                             ccgcTTCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTcc        <  1:350877/140‑1 (MQ=255)

                                                                                                                                  tCCAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATtgt   >  1:823101/1‑140 (MQ=255)

                                                                                                                                   ccAACGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATtgtg  <  2:497619/140‑1 (MQ=255)

GCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTG  >  NC_000913/4028506‑4028776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A