Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F28 I1 R1 182 57.1 1926220 99.1% 1908884 138.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,084,255 Δ1 bp coding (1568/3051 nt) fdoG ← formate dehydrogenase O subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,084,2520C.100.0% 149.4 / NA 36coding (1571/3051 nt)fdoGformate dehydrogenase O subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (15/21);  total (15/21)

ATCACTTTGTTTTTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAGCCCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGT  >  NC_000913/4084114‑4084385
                                                                                                                                          |                                                                                                                                     
aTCACTTTGTTTTTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAGcc                                                                                                                                      <  2:430023/140‑1 (MQ=255)
   aCTTTGTTTTTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAGcccag                                                                                                                                   >  2:362488/1‑138 (MQ=255)
               ttCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTccgcc                                                                                                                      <  2:412827/140‑1 (MQ=255)
                        gAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTa                                                                                                             <  1:406188/140‑1 (MQ=255)
                          ggCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATc                                                                                                           <  1:289062/140‑1 (MQ=255)
                           gCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCa                                                                                                          <  2:507161/140‑1 (MQ=255)
                             aaCAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCAcc                                                                                                        >  2:879314/1‑140 (MQ=255)
                              aCAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCa                                                                                                       >  1:244775/1‑140 (MQ=255)
                              aCAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCa                                                                                                       >  1:586497/1‑140 (MQ=255)
                                      aaaGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGc                                                                                               <  2:586497/140‑1 (MQ=255)
                                      aaaGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGc                                                                                               <  2:244775/140‑1 (MQ=255)
                                         gCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCtt                                                                                            <  1:362488/140‑1 (MQ=255)
                                         gCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCtt                                                                                            <  1:17848/140‑1 (MQ=255)
                                            cTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTtcat                                                                                         >  1:369608/1‑140 (MQ=255)
                                                   atatAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATagag                                                                                  <  1:293754/140‑1 (MQ=255)
                                                         ccATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCtt                                                                                            >  1:230390/1‑124 (MQ=255)
                                                         ccATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCtt                                                                                            <  2:230390/124‑1 (MQ=255)
                                                         ccATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACgaa                                                                             <  1:410943/139‑1 (MQ=255)
                                                         ccATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACgaa                                                                             >  2:410943/1‑139 (MQ=255)
                                                             tGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAAtt                                                                        >  1:47667/1‑140 (MQ=255)
                                                                   tGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGt                                                                  >  1:917929/1‑140 (MQ=255)
                                                                         ctTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGc                                                            >  1:504436/1‑140 (MQ=255)
                                                                          tttCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAAtt                                                                        >  1:376104/1‑127 (MQ=255)
                                                                          tttCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAAtt                                                                        <  2:376104/127‑1 (MQ=255)
                                                                          tttCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTg                                                             >  2:664357/1‑138 (MQ=255)
                                                                          tttCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTg                                                             <  1:664357/138‑1 (MQ=255)
                                                                          tttCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGcc                                                           >  2:384904/1‑140 (MQ=255)
                                                                            tcatcaTCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGcccc                                                         <  1:879314/140‑1 (MQ=255)
                                                                            tcatcaTCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGcccc                                                         <  1:245127/140‑1 (MQ=255)
                                                                            tcatcaTCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGcccc                                                         <  2:369608/140‑1 (MQ=255)
                                                                               tcatcTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCagt                                                      >  2:721585/1‑140 (MQ=255)
                                                                                 atcTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCagtag                                                    <  1:721585/140‑1 (MQ=255)
                                                                                        aGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCt                                             <  2:731560/140‑1 (MQ=255)
                                                                                        aGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAATAGTTTACCt                                             <  2:504436/140‑1 (MQ=255)
                                                                                           aTTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGc                                          >  2:18591/1‑140 (MQ=255)
                                                                                                                 aTCCCACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCgt                    >  2:766683/1‑140 (MQ=255)
                                                                                                                   cccACTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCgtgt                  <  1:766683/140‑1 (MQ=255)
                                                                                                                       cTTCGGCAACCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGc              >  2:421566/1‑140 (MQ=255)
                                                                                                                               aCCAGTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAaggt      <  1:18591/140‑1 (MQ=255)
                                                                                                                                   gTCAAAG‑CCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAaggtaggt  <  1:421566/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                     
ATCACTTTGTTTTTGTTCGGGAATGAGGCAACAGGGTTAAAGCCCTGGCAGATATAGCCATTGACCTTGCCCTCTTTCATCATCTCGAAGTATTGCAGGACGTCGTAGCCTTTATCCCACTTCGGCAACCAGTCAAAGCCCCAGCTATTTTCCGCCGTCGCTTTATCACCAAAGAAGGCCTTCATCATAGAGACGAAGAATTTCGGGTAGTTGCCCCAGTAGTTTACCTGGCCTTCCAGCAGCGGTTTTGGCGTGTTGGCGGTAAGGTAGGT  >  NC_000913/4084114‑4084385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A