Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F28 I1 R1 182 57.1 1926220 99.1% 1908884 138.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,277,869 A→G N134S (AAC→AGC)  soxR → DNA‑binding transcriptional dual regulator SoxR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,277,8690AG100.0% 134.1 / NA 38N134S (AAC→AGC) soxRDNA‑binding transcriptional dual regulator SoxR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/17);  total (21/17)

GTGGAAACAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGG  >  NC_000913/4277738‑4278001
                                                                                                                                   |                                                                                                                                    
gTGGAAACAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCg                                                                                                                              <  1:907128/140‑1 (MQ=255)
 tGGAAACAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGa                                                                                                                             <  1:574795/140‑1 (MQ=255)
         gCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAg                                                                                                                     >  2:195949/1‑140 (MQ=255)
            ttCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGgag                                                                                                                  <  1:633685/140‑1 (MQ=255)
              cGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGagaa                                                                                                                <  2:52126/140‑1 (MQ=255)
                             aagaGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGCCGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCa                                                                                                 <  1:384950/140‑1 (MQ=255)
                               gagTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACg                                                                                               <  1:958308/140‑1 (MQ=255)
                                          gcgcATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAg                                                                                    >  1:337839/1‑140 (MQ=255)
                                          gcgcATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAg                                                                                    <  2:730095/140‑1 (MQ=255)
                                                tCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAAc                                                                              >  1:559542/1‑140 (MQ=255)
                                                tCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAAc                                                                              <  2:337839/140‑1 (MQ=255)
                                                    aCCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACaaaa                                                                          >  1:631545/1‑140 (MQ=255)
                                                         aGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTaaa                                                                     >  1:646430/1‑140 (MQ=255)
                                                          gTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAg                                                                    >  2:390244/1‑140 (MQ=255)
                                                              cgcTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAg                                                                    <  2:519990/136‑1 (MQ=255)
                                                              cgcTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAg                                                                    >  1:519990/1‑136 (MQ=255)
                                                               gcTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTa                                                                       <  2:713730/132‑1 (MQ=255)
                                                               gcTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTa                                                                       >  1:713730/1‑132 (MQ=255)
                                                                cTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGTAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCcac                                                              >  2:481933/1‑140 (MQ=255)
                                                                   cGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAg                                                           <  1:390244/140‑1 (MQ=255)
                                                                               gACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAgtt                                               >  1:894938/1‑140 (MQ=255)
                                                                                     tGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGtttt                                         >  2:767888/1‑140 (MQ=255)
                                                                                      gTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTc                                        <  1:167123/140‑1 (MQ=255)
                                                                                         ttGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCgg                                     >  1:34115/1‑140 (MQ=255)
                                                                                            gTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtct                                  >  2:218998/1‑140 (MQ=255)
                                                                                                tgGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtct                                  >  1:4976/1‑136 (MQ=255)
                                                                                                tgGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtct                                  <  2:4976/136‑1 (MQ=255)
                                                                                                 ggCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttgtc                             >  2:375129/1‑140 (MQ=255)
                                                                                                   cTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttgtctt                           >  2:817968/1‑140 (MQ=255)
                                                                                                      ccTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttg                               >  1:699469/1‑133 (MQ=255)
                                                                                                      ccTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttg                               <  2:699469/133‑1 (MQ=255)
                                                                                                      ccTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTtct                        >  2:904361/1‑140 (MQ=255)
                                                                                                               cAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATccc               <  1:904361/140‑1 (MQ=255)
                                                                                                                 gTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGc             <  2:34115/140‑1 (MQ=255)
                                                                                                                            ttGCGTAGCCCGGGCGACCGCTTAGGATAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTg           <  2:7147/131‑1 (MQ=255)
                                                                                                                            ttGCGTAGCCCGGGCGACCGCTTAGGATAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTg           >  1:7147/1‑131 (MQ=255)
                                                                                                                            ttGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACagg  >  1:957287/1‑140 (MQ=255)
                                                                                                                            ttGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTGTCCGGTCTTTGTCGTTCTCTCTATCCCGCTGGTAGACagg  >  2:851464/1‑140 (MQ=255)
                                                                                                                                   |                                                                                                                                    
GTGGAAACAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGG  >  NC_000913/4277738‑4278001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A