Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F28 I1 R1 182 57.1 1926220 99.1% 1908884 138.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,460,176 A→G R63R (CGT→CGC)  nrdG ← anaerobic ribonucleoside‑triphosphate reductase activating protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,460,1760AG100.0% 134.3 / NA 40R63R (CGT→CGC) nrdGanaerobic ribonucleoside‑triphosphate reductase activating protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/24);  total (16/24)

GTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAAC  >  NC_000913/4460040‑4460312
                                                                                                                                        |                                                                                                                                        
gTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGa                                                                                                                                       <  1:195992/140‑1 (MQ=255)
gTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGa                                                                                                                                       <  1:94288/140‑1 (MQ=255)
  ttATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAgt                                                                                                                                     <  1:951472/140‑1 (MQ=255)
   tataGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAgtg                                                                                                                                    <  1:102792/140‑1 (MQ=255)
        ccTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCAtt                                                                                                                               <  1:730850/140‑1 (MQ=255)
          tGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCa                                                                                                                             <  1:947423/140‑1 (MQ=255)
            tCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGa                                                                                                                           >  2:526675/1‑140 (MQ=255)
            tCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGa                                                                                                                           <  1:484970/140‑1 (MQ=255)
             ccACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGAt                                                                                                                          <  1:759695/140‑1 (MQ=255)
                 acCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCAtt                                                                                                                               >  1:187021/1‑131 (MQ=255)
                 acCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCAtt                                                                                                                               <  2:187021/131‑1 (MQ=255)
                       aTGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGAt                                                                                                                <  1:284533/140‑1 (MQ=255)
                           cTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTgg                                                                                                            >  1:199860/1‑140 (MQ=255)
                                    gACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGTTGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCAtt                                                                                                   >  2:638574/1‑140 (MQ=255)
                                    gACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATg                                                                                                                  >  2:719397/1‑125 (MQ=255)
                                    gACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATg                                                                                                                  <  1:719397/125‑1 (MQ=255)
                                        cTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCtt                                                                                               <  2:604459/140‑1 (MQ=255)
                                        cTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCtt                                                                                               <  1:943218/140‑1 (MQ=255)
                                                  aTGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATgg                                                                                     <  1:638574/140‑1 (MQ=255)
                                                   tGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGc                                                                                    >  1:518247/1‑140 (MQ=255)
                                                   tGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGc                                                                                    <  1:526675/140‑1 (MQ=255)
                                                        tGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGAcc                                                                               <  1:297160/140‑1 (MQ=255)
                                                            ccAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGaa                                                                           >  1:407104/1‑140 (MQ=255)
                                                            ccAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGaa                                                                           >  2:64481/1‑140 (MQ=255)
                                                                  tCAGAATATCCGGCCCGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACAGGAATTTAcc                                                                     <  2:518247/140‑1 (MQ=255)
                                                                          tCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCAtgt                                                             >  2:347631/1‑140 (MQ=255)
                                                                           ccGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGCCTGACCGGAATTTACCCGCCAtgtg                                                            >  2:70306/1‑140 (MQ=255)
                                                                             ggCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCt                                                          >  1:360231/1‑140 (MQ=255)
                                                                                aCGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCtttt                                                       <  2:407104/140‑1 (MQ=255)
                                                                                        cGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGc                                               <  1:64481/140‑1 (MQ=255)
                                                                                                gCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCgggg                                       <  2:80879/140‑1 (MQ=255)
                                                                                                         cgccgGAGAGGGAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGa                              >  1:370793/1‑140 (MQ=255)
                                                                                                                  gggAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCg                     <  1:347631/140‑1 (MQ=255)
                                                                                                                   ggAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCgg                    >  2:862278/1‑140 (MQ=255)
                                                                                                                    gAAATACCCTGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGga                   <  2:199860/140‑1 (MQ=255)
                                                                                                                             tGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAAc            >  2:684475/1‑138 (MQ=255)
                                                                                                                             tGGCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAAc            <  1:684475/138‑1 (MQ=255)
                                                                                                                              ggCGTTTGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAgg         >  1:782112/1‑140 (MQ=255)
                                                                                                                                  tttGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGc     >  1:53358/1‑140 (MQ=255)
                                                                                                                                    tGATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCaa   <  1:457709/140‑1 (MQ=255)
                                                                                                                                     gATGCGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAAc  <  2:370793/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GTTTATAGCCTGTCCACACCCAGATGTCTTTACCCGGACACTCGGCGCGGATGCGTTGTACCAGTTTCAGAATATCCGGCACGTTTTGCGGATGCAGCGGATCGCCGCCGGAGAGGGAAATACCCTGGCGTTTGATACGAGTGTCATTCAGATCGTTAATGATCTGGTCTTCCATTGCTTTGGTAAATGGCTGACCGGAATTTACCCGCCATGTGCTTTTGTTATAGCAACCGGGGCATTCATGAACACACCCGGAGACAAACAGGGTGCAAC  >  NC_000913/4460040‑4460312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A