Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	162	86.7	2935410	99.3%	2914862	138.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,462,897	(T)_7→8	intergenic (‑237/+157)	nrdD ← / ← treC	anaerobic ribonucleoside‑triphosphate reductase/trehalose‑6‑phosphate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,462,890	1	.	T	97.3%	257.6 / ‑0.9	73	intergenic (‑230/+164)	nrdD/treC	anaerobic ribonucleoside‑triphosphate reductase/trehalose‑6‑phosphate hydrolase
Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (50/21); total (51/22)
Fisher's exact test for biased strand distribution p-value = 5.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

TCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGC‑TTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTGACAGACAAAACCCCGG  >  NC_000913/4462753‑4463021

tCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGC‑tt                                                                                                                                   <  1:1308075/140‑1 (MQ=255)

   ccATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGC‑ttttt                                                                                                                                <  2:812313/140‑1 (MQ=255)

                    aCGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAg                                                                                                                >  1:1259529/1‑140 (MQ=255)

                     cgccgcACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGt                                                                                                               >  1:364176/1‑140 (MQ=255)

                        cgcACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTaaa                                                                                                            >  2:224679/1‑140 (MQ=255)

                        cgcACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGC‑TTTTTTTGATGTAGCTCAAAGTaaaa                                                                                                           >  1:489510/1‑140 (MQ=255)

                          cACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTaaaaa                                                                                                          >  2:1140907/1‑140 (MQ=255)

                            ccTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGC‑TTTTTTTGATGTAGCTCAAAGTAAAAAGCa                                                                                                       <  2:489510/140‑1 (MQ=255)

                             cTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCa                                                                                                       <  2:110597/140‑1 (MQ=255)

                             cTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCa                                                                                                       <  2:1243054/140‑1 (MQ=255)

                             cTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCa                                                                                                       <  1:1365706/140‑1 (MQ=255)

                               ttttCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCt                                                                                                                     <  2:547253/124‑1 (MQ=255)

                               ttttCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCt                                                                                                                     >  1:547253/1‑124 (MQ=255)

                                            cTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGAc                                                                                        <  1:1422025/140‑1 (MQ=255)

                                                    gCAATTATAGTCGATTGATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTa                                                                                >  2:1292854/1‑140 (MQ=255)

                                                       aTTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAg                                                                             >  2:1142250/1‑140 (MQ=255)

                                                       aTTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAg                                                                             >  1:1310395/1‑140 (MQ=255)

                                                         tataGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCg                                                                           >  1:172445/1‑140 (MQ=255)

                                                         tataGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCg                                                                           >  1:683886/1‑140 (MQ=255)

                                                         tataGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCg                                                                           >  2:972175/1‑140 (MQ=255)

                                                              tCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATg                                                                      >  1:1192603/1‑140 (MQ=255)

                                                                  ttAATACAACATATTGGGTTGGGACGCA‑TTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAAt                                                                 >  2:1093985/1‑140 (MQ=255)

                                                                         aacaTATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTa                                                           <  2:1259529/140‑1 (MQ=255)

                                                                           caTATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAAt                                                         >  1:1401287/1‑140 (MQ=255)

                                                                                tgggttgggACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAAtt                                                    >  2:1292914/1‑140 (MQ=255)

                                                                                tgggttgggACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAAtt                                                    >  2:946526/1‑140 (MQ=255)

                                                                                tgggttgggACACGTTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAAtt                                                    >  1:1287186/1‑140 (MQ=255)

                                                                                     tgggACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTg                                               >  1:351729/1‑140 (MQ=255)

                                                                                      gggACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGc                                                 <  1:495295/137‑1 (MQ=255)

                                                                                      gggACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGc                                                 >  2:495295/1‑137 (MQ=255)

                                                                                      gggACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTgg                                              >  2:279827/1‑140 (MQ=255)

                                                                                      gggACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTgg                                              >  1:1158221/1‑140 (MQ=255)

                                                                                       ggACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGt                                             >  2:1073576/1‑140 (MQ=255)

                                                                                       ggACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGt                                             >  1:541183/1‑140 (MQ=255)

                                                                                             aTTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGaaaa                                       >  1:677889/1‑140 (MQ=255)

                                                                                                 tAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATca                                   <  1:279827/140‑1 (MQ=255)

                                                                                                   aaGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATcaac                                 <  2:33450/140‑1 (MQ=255)

                                                                                                   aaGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATcaac                                 <  2:683886/140‑1 (MQ=255)

                                                                                                     gTCTATATATAGTGCTTCGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATcaacaa                               <  2:1310395/140‑1 (MQ=255)

                                                                                                         atatataGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAaac                           <  2:33884/140‑1 (MQ=255)

                                                                                                               aGTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGGCGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTG‑AAAATCAACAAAAACAACATAc                    >  1:458184/1‑140 (MQ=255)

                                                                                                                gTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACaaa                              >  1:331713/1‑130 (MQ=255)

                                                                                                                gTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACaaa                              <  2:331713/130‑1 (MQ=255)

                                                                                                                gTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATAc                    >  2:567913/1‑140 (MQ=255)

                                                                                                                gTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATAc                    >  2:708026/1‑140 (MQ=255)

                                                                                                                gTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATAc                    >  2:741532/1‑140 (MQ=255)

                                                                                                                gTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATAc                    >  2:795918/1‑140 (MQ=255)

                                                                                                                gTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATAc                    >  1:1159778/1‑140 (MQ=255)

                                                                                                                gTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATAc                    >  1:111413/1‑140 (MQ=255)

                                                                                                                gTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATAc                    >  2:977367/1‑140 (MQ=255)

                                                                                                                gTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATAc                    >  1:647645/1‑140 (MQ=255)

                                                                                                                gTGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATAc                    >  1:35963/1‑140 (MQ=255)

                                                                                                                 tGCTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACt                   >  1:733484/1‑140 (MQ=255)

                                                                                                                   cTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATcaac                                 <  2:1276344/124‑1 (MQ=255)

                                                                                                                   cTTTGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATcaac                                 >  1:1276344/1‑124 (MQ=255)

                                                                                                                    tttGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTgac                >  2:615380/1‑140 (MQ=255)

                                                                                                                    tttGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTgac                >  1:1223446/1‑140 (MQ=255)

                                                                                                                    tttGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTgac                >  2:784933/1‑140 (MQ=255)

                                                                                                                    tttGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTgac                >  1:674837/1‑140 (MQ=255)

                                                                                                                    tttGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTgac                >  1:1416221/1‑140 (MQ=255)

                                                                                                                    tttGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTgac                >  1:423795/1‑140 (MQ=255)

                                                                                                                    tttGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTgac                <  2:351729/140‑1 (MQ=255)

                                                                                                                    tttGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTgac                >  2:20350/1‑140 (MQ=255)

                                                                                                                     ttGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTgaca               <  1:708026/140‑1 (MQ=255)

                                                                                                                      tGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTgacag              >  2:531325/1‑140 (MQ=255)

                                                                                                                      tGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTgacag              >  2:13877/1‑140 (MQ=255)

                                                                                                                      tGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTgacag              >  2:958023/1‑140 (MQ=255)

                                                                                                                      tGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTga                 >  2:533718/1‑137 (MQ=255)

                                                                                                                      tGCATCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTga                 <  1:533718/137‑1 (MQ=255)

                                                                                                                         aTCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTgacagaca           <  2:677889/140‑1 (MQ=255)

                                                                                                                         aTCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTgacagaca           <  2:364176/140‑1 (MQ=255)

                                                                                                                         aTCAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTgacagaca           <  2:172445/140‑1 (MQ=255)

                                                                                                                           cAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAaac                           >  2:791538/1‑122 (MQ=255)

                                                                                                                           cAAGGATGTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAaac                           <  1:791538/122‑1 (MQ=255)

                                                                                                                                  gTTTGAGCTTTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTGACAGACAAAACCCCgg  >  2:1098308/1‑140 (MQ=255)

TCGCCATCCGCTTATCCACAACGCCGCACCTTTTTCGTGGGGAGCTGTAGTAGCAATTATAGTCGATTAATACAACATATTGGGTTGGGACGCATTTTAAAGTCTATATATAGTGCTTTGCATCAAGGATGTTTGAGC‑TTTTTTTGATGTAGCTCAAAGTAAAAAGCAGAGCGTACGGATGACGGGCGCTACAGCGATATGTAAATTTTTTAATGAATTTGCTGGTTGAAAAATCAACAAAAACAACATACTGACAGACAAAACCCCGG  >  NC_000913/4462753‑4463021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A