Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	182	57.1	1926220	99.1%	1908884	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,596,841	C→T	S284S (AGC→AGT)	lgoD →	L‑galactonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,596,841	0	C	T	100.0%	154.6 / NA	45	S284S (AGC→AGT)	lgoD	L‑galactonate oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (23/22); total (23/22)

GTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAGCGCGATGTGATTAA  >  NC_000913/4596705‑4596975

gTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGc                                                                                                                                     <  1:934225/140‑1 (MQ=255)

       aCATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAacgcca                                                                                                                              >  2:674344/1‑140 (MQ=255)

       aCATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAacgcca                                                                                                                              <  2:653928/140‑1 (MQ=255)

          tGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAacgccacgc                                                                                                                           >  2:164665/1‑140 (MQ=255)

          tGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAacgccacgc                                                                                                                           >  1:51017/1‑140 (MQ=255)

          tGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAacgccacgc                                                                                                                           >  1:912135/1‑140 (MQ=255)

             gATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCgg                                                                                                                        <  2:594318/140‑1 (MQ=255)

                  aTAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGAt                                                                                                                   >  2:85942/1‑140 (MQ=255)

                   tAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGAtt                                                                                                                  >  2:608515/1‑140 (MQ=255)

                         cGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTa                                                                                                            >  2:45250/1‑140 (MQ=255)

                              aTTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAgtc                                                                                                       >  1:575028/1‑140 (MQ=255)

                               tttGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAgtcg                                                                                                      <  1:164665/140‑1 (MQ=255)

                                ttGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAgtcgg                                                                                                     <  1:14421/140‑1 (MQ=255)

                                 tGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAgtcggt                                                                                                    <  2:928372/140‑1 (MQ=255)

                                 tGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAgtcggt                                                                                                    <  2:51017/140‑1 (MQ=255)

                                                                cGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAAt                                                                             >  2:844615/1‑132 (MQ=255)

                                                                cGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAAt                                                                             <  1:844615/132‑1 (MQ=255)

                                                                cGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCActgctg                                                                     >  2:120585/1‑140 (MQ=255)

                                                                  gCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGCTGGCGGAAGGAAAAATCACTGCTGAc                                                                   >  1:99507/1‑140 (MQ=255)

                                                                  gCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGAc                                                                   >  1:560856/1‑140 (MQ=255)

                                                                   ccTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACa                                                                  <  1:85942/140‑1 (MQ=255)

                                                                    cTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACat                                                                 <  1:674344/140‑1 (MQ=255)

                                                                       tttAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACa                                                                  >  2:300684/1‑136 (MQ=255)

                                                                       tttAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACa                                                                  <  1:300684/136‑1 (MQ=255)

                                                                              gTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAAcc                                                       <  1:45250/140‑1 (MQ=255)

                                                                                 aGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACa                                                                  <  2:130143/126‑1 (MQ=255)

                                                                                 aGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACa                                                                  >  1:130143/1‑126 (MQ=255)

                                                                                 aGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAAc                                                        <  1:501734/136‑1 (MQ=255)

                                                                                 aGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAAc                                                        >  2:501734/1‑136 (MQ=255)

                                                                                 aGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCAt                                                    >  1:829212/1‑140 (MQ=255)

                                                                                 aGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCAt                                                    >  1:285695/1‑140 (MQ=255)

                                                                                 aGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCAt                                                    >  1:441691/1‑140 (MQ=255)

                                                                                 aGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCAt                                                    >  2:299424/1‑140 (MQ=255)

                                                                                     gCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCt                                                >  2:624808/1‑140 (MQ=255)

                                                                                     gCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCt                                                <  2:135401/140‑1 (MQ=255)

                                                                                               gATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGc                                      <  1:608515/140‑1 (MQ=255)

                                                                                                    ggAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGc                                 <  1:299424/140‑1 (MQ=255)

                                                                                                       aTTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTgg                              <  1:624808/140‑1 (MQ=255)

                                                                                                             tAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGaaa                        >  1:476344/1‑140 (MQ=255)

                                                                                                               aaaaaGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAAcc                      >  2:374723/1‑140 (MQ=255)

                                                                                                                 aaaGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTa                    <  2:329865/140‑1 (MQ=255)

                                                                                                                           cgaTGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAGCGCGAt          <  1:637593/140‑1 (MQ=255)

                                                                                                                            gatgatGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAGcgcg            >  1:708036/1‑137 (MQ=255)

                                                                                                                            gatgatGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAGcgcg            <  2:708036/137‑1 (MQ=255)

                                                                                                                                   ggCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAGCGCGATGTGATTaa  <  2:829212/140‑1 (MQ=255)

GTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAGCGCGATGTGATTAA  >  NC_000913/4596705‑4596975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A