Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	128	54.7	1845674	99.3%	1832754	139.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	685,011	A→G	V82A (GTA→GCA)	gltL ←	glutamate/aspartate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	685,011	0	A	G	100.0%	132.1 / NA	36	V82A (GTA→GCA)	gltL	glutamate/aspartate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/19); total (17/19)

AGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGA  >  NC_000913/684879‑685135

aGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATccc                                                                                                                       <  2:86737/140‑1 (MQ=255)

    cGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGAcg                                                                                                                   >  2:233597/1‑140 (MQ=255)

        acGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCggg                                                                                                               >  2:368557/1‑140 (MQ=255)

        acGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCggg                                                                                                               >  1:892071/1‑140 (MQ=255)

        acGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGACAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGAcgc                                                                                                                  <  1:213709/137‑1 (MQ=255)

        acGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGACAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGAcgc                                                                                                                  >  2:213709/1‑137 (MQ=255)

           cTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAAc                                                                                                            <  1:233597/140‑1 (MQ=255)

           cTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAAc                                                                                                            <  2:187448/140‑1 (MQ=255)

                  agTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCtt                                                                                                     <  1:368557/140‑1 (MQ=255)

                      tCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTAGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGcc                                                                                                 >  2:197180/1‑140 (MQ=255)

                          ggCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGAt                                                                                             <  1:452135/140‑1 (MQ=255)

                               tttCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGtt                                                                                        >  1:770169/1‑140 (MQ=255)

                                  cACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTc                                                                                     <  2:112523/140‑1 (MQ=255)

                                          ggCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCTGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGtt                                                                             <  1:590023/140‑1 (MQ=255)

                                          ggCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGtt                                                                             <  2:518449/140‑1 (MQ=255)

                                               tttATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCa                                                                        <  1:503309/140‑1 (MQ=255)

                                                 tATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACg                                                                      <  1:859704/140‑1 (MQ=255)

                                                 tATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACg                                                                      <  2:342781/140‑1 (MQ=255)

                                                 tATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACg                                                                      >  2:258533/1‑140 (MQ=255)

                                                     gcgTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATAc                                                                  <  2:303914/140‑1 (MQ=255)

                                                            aGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGAc                                                           <  2:216025/140‑1 (MQ=255)

                                                             gCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACg                                                          <  2:770169/140‑1 (MQ=255)

                                                                          cgcCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCtt                                             >  2:684252/1‑140 (MQ=255)

                                                                           gcCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTtg                                            <  1:684252/140‑1 (MQ=255)

                                                                           gcCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTtg                                            >  1:813471/1‑140 (MQ=255)

                                                                                 gTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCAc                                      <  1:506912/140‑1 (MQ=255)

                                                                                           cGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAgg                            >  2:495117/1‑140 (MQ=255)

                                                                                                  cGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGa                     <  2:813471/140‑1 (MQ=255)

                                                                                                  cGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGa                     <  2:841537/140‑1 (MQ=255)

                                                                                                           aGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTaa            >  2:100709/1‑140 (MQ=255)

                                                                                                            gggAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCGTTGCCAGATCGGTTTTCTTGTCGTTAGCCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAAt           <  2:97746/140‑1 (MQ=255)

                                                                                                             ggAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATc          >  2:751922/1‑140 (MQ=255)

                                                                                                                  aGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACTGTTTTAATCAGCGt     >  1:555203/1‑140 (MQ=255)

                                                                                                                  aGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGt     >  1:827942/1‑140 (MQ=255)

                                                                                                                   gCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGtt    >  1:335783/1‑140 (MQ=255)

                                                                                                                    cTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTg   >  1:91903/1‑140 (MQ=255)

                                                                                                                     tCGAAATGCTGGAATGCCATCACGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGa  >  2:654446/1‑140 (MQ=255)

AGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGA  >  NC_000913/684879‑685135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A