Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	128	54.7	1845674	99.3%	1832754	139.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,017,609	A→G	D517G (GAC→GGC)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,017,609	0	A	G	100.0%	130.8 / NA	36	D517G (GAC→GGC)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/9); total (27/9)

ATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGC  >  NC_000913/3017479‑3017741

aTCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGAGCGCt                                                                                                                             >  2:816044/1‑140 (MQ=255)

       tGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATc                                                                                                                      >  1:478569/1‑140 (MQ=255)

                 acacCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGa                                                                                                            >  1:168869/1‑140 (MQ=255)

                      gCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGaaaaaa                                                                                                       >  2:687253/1‑140 (MQ=255)

                                            cATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTggg                                                                                 >  2:775183/1‑140 (MQ=255)

                                             aTCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGa                                                                                >  2:482320/1‑140 (MQ=255)

                                                 aCCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGaa                                                                            >  1:677623/1‑140 (MQ=255)

                                                  ccAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAat                                                                           >  2:108110/1‑140 (MQ=255)

                                                   caacgcaacgCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAata                                                                          >  2:786157/1‑140 (MQ=255)

                                                     acgcaacgcTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAAtata                                                                        >  1:496216/1‑140 (MQ=255)

                                                       gcaacgcTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAg                                                                      >  1:45296/1‑140 (MQ=255)

                                                       gcaacgcTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAg                                                                      >  2:222220/1‑140 (MQ=255)

                                                                tGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTgg                                                             >  1:707669/1‑140 (MQ=255)

                                                                    cGCCATTCCCGGTAATATTTGCGACCCCCAGTCCCAATACACCTGTCCCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGcaca                                                         <  2:316752/140‑1 (MQ=255)

                                                                            ccGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGcc                                                 >  1:262514/1‑140 (MQ=255)

                                                                               gTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGt                                              >  1:658682/1‑140 (MQ=255)

                                                                                taatttttGCGATCACCAGTGCAAATACAACTGTCCCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGtt                                             <  1:52048/135‑1 (MQ=255)

                                                                                aattttttGCGACCACCAGTGCAAACACAACTGTCCCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGtt                                             <  2:278134/135‑1 (MQ=255)

                                                                                 cATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTc                                            >  2:331748/1‑140 (MQ=255)

                                                                                 cATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTc                                            >  1:722854/1‑140 (MQ=255)

                                                                                    atTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTgg                                         >  1:347466/1‑140 (MQ=255)

                                                                                        gCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCa                                     <  1:331748/140‑1 (MQ=255)

                                                                                          gATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACg                                   >  1:717492/1‑140 (MQ=255)

                                                                                          gATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACg                                   >  2:461276/1‑140 (MQ=255)

                                                                                              aCCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGcctg                               >  1:278134/1‑138 (MQ=255)

                                                                                                   tGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGt                          >  1:180053/1‑140 (MQ=255)

                                                                                                       aaTACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGcc                      <  1:222220/140‑1 (MQ=255)

                                                                                                        aTACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCg                     >  1:864701/1‑140 (MQ=255)

                                                                                                         tACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGt                    >  1:391843/1‑140 (MQ=255)

                                                                                                            aaCTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGAt                 <  1:60765/140‑1 (MQ=255)

                                                                                                            aaCTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGAt                 <  2:707669/140‑1 (MQ=255)

                                                                                                             aCTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGAtt                <  2:347466/140‑1 (MQ=255)

                                                                                                                  aCCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGc           <  2:478569/140‑1 (MQ=255)

                                                                                                                     cGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAgg        >  2:788407/1‑140 (MQ=255)

                                                                                                                      gCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGt       >  2:102732/1‑140 (MQ=255)

                                                                                                                           gATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCcggc  <  1:566204/140‑1 (MQ=255)

                                                                                                                           gATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCcggc  >  2:308998/1‑140 (MQ=255)

ATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGC  >  NC_000913/3017479‑3017741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A