Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F28 I1 R1 116 72.9 2471836 99.3% 2454533 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,449,264 A→T I207N (ATC→AAC)  rpsC ← 30S ribosomal subunit protein S3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,449,2640AT97.0% 225.6 / ‑2.2 67I207N (ATC→AAC) rpsC30S ribosomal subunit protein S3
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base T (30/35);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

TTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGATCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCAGAGTGTGCAGCGGTACGCGACCTTCGCGGTACCATTCGGTACGTGCGAT  >  NC_000913/3449128‑3449400
                                                                                                                                        |                                                                                                                                        
tttGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTtct                                                                                                                                       <  2:868023/140‑1 (MQ=255)
tttGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTtct                                                                                                                                       <  1:51042/140‑1 (MQ=255)
  tgtgCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAGCAGCAGCCATACCACCCAGGTTCTCg                                                                                                                                     >  1:863089/1‑140 (MQ=255)
  tgtgCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCg                                                                                                                                     <  1:1092667/140‑1 (MQ=255)
  tgtgCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCg                                                                                                                                     >  1:1185136/1‑140 (MQ=255)
        ttttACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTg                                                                                                                               <  1:1080071/140‑1 (MQ=255)
        ttttACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTg                                                                                                                               >  1:400731/1‑140 (MQ=255)
        ttttACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTg                                                                                                                               >  1:86696/1‑140 (MQ=255)
           tACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAg                                                                                                                            <  1:787548/140‑1 (MQ=255)
                aaTTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCca                                                                                                                       <  1:285657/140‑1 (MQ=255)
                         cGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAAc                                                                                                              <  2:516004/140‑1 (MQ=255)
                         cGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAAc                                                                                                              <  1:791747/140‑1 (MQ=255)
                              tGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGa                                                                                                         >  2:68761/1‑140 (MQ=255)
                              tGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGa                                                                                                         >  1:945500/1‑140 (MQ=255)
                              tGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGa                                                                                                         <  1:981131/140‑1 (MQ=255)
                               ggTTGTAACATCAGCGACGCTCCTTATTTTCGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGAt                                                                                                        <  2:863089/140‑1 (MQ=255)
                                gTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGAtt                                                                                                       <  1:1224442/140‑1 (MQ=255)
                                gTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGAtt                                                                                                       <  2:63902/140‑1 (MQ=255)
                                  tGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTac                                                                                                     <  2:223653/140‑1 (MQ=255)
                                  tGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTac                                                                                                     <  1:375404/140‑1 (MQ=255)
                                     aaCATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACAcc                                                                                                  >  1:522366/1‑140 (MQ=255)
                                          cAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGATCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAg                                                                                             >  1:1186515/1‑140 (MQ=255)
                                           aGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAgt                                                                                            >  1:1015643/1‑140 (MQ=255)
                                               aCGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAgtggtg                                                                                        >  2:62351/1‑140 (MQ=255)
                                               aCGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAgtggtg                                                                                        >  2:298162/1‑140 (MQ=255)
                                                 gCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGtgtg                                                                                      <  1:62351/140‑1 (MQ=255)
                                                 gCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGtgtg                                                                                      <  1:414769/140‑1 (MQ=255)
                                                  cTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTgc                                                                                     >  1:836718/1‑140 (MQ=255)
                                                  cTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTgc                                                                                     >  1:156609/1‑140 (MQ=255)
                                                  cTCCTTATTTACGGCCTTTACGCTGCTGC‑‑TTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGtgtg                                                                                      <  2:241306/137‑1 (MQ=255)
                                                  cTCCTTATTTACGGCCTTTACGCTGCTGC‑‑TTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGtgtg                                                                                      >  1:241306/1‑137 (MQ=255)
                                                          ttACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCaga                                                                             <  2:683142/140‑1 (MQ=255)
                                                               gCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGGAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGgtgt                                                                        >  2:729595/1‑140 (MQ=255)
                                                               gCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGgtgt                                                                        >  2:624499/1‑140 (MQ=255)
                                                               gCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGgtgt                                                                        >  2:78263/1‑140 (MQ=255)
                                                                    tACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAg                                                                   >  2:896971/1‑140 (MQ=255)
                                                                     aCGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGt                                                                  <  1:521029/140‑1 (MQ=255)
                                                                     aCGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGATCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGt                                                                  <  2:1186515/140‑1 (MQ=255)
                                                                        ctgctgctTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGgtgt                                                                        <  1:231692/131‑1 (MQ=255)
                                                                        ctgctgctTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGgtgt                                                                        >  2:231692/1‑131 (MQ=255)
                                                                           ctgctTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCagag                                                                            >  1:154736/1‑124 (MQ=255)
                                                                           ctgctTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCagag                                                                            <  2:154736/124‑1 (MQ=255)
                                                                               tttttAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGc                                                        <  2:86696/140‑1 (MQ=255)
                                                                                 tttAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCAc                                                      <  2:1015643/140‑1 (MQ=255)
                                                                                  ttAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACg                                                     <  1:297451/140‑1 (MQ=255)
                                                                                     ggCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCag                                                  >  2:452381/1‑140 (MQ=255)
                                                                                      gCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCaga                                                 <  1:1073654/140‑1 (MQ=255)
                                                                                      gCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCaga                                                 >  2:951037/1‑140 (MQ=255)
                                                                                      gCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCaga                                                 <  1:452381/140‑1 (MQ=255)
                                                                                      gCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCaga                                                 <  2:522366/140‑1 (MQ=255)
                                                                                         gAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAg                                                         <  2:1148418/129‑1 (MQ=255)
                                                                                         gAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAg                                                         >  1:1148418/1‑129 (MQ=255)
                                                                                          agcagcCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCAGAgtgt                                             >  1:1075267/1‑140 (MQ=255)
                                                                                          agcagcCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCAGAgtgt                                             >  2:995218/1‑140 (MQ=255)
                                                                                          agcagcCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCAGAgtgt                                             >  1:486170/1‑140 (MQ=255)
                                                                                          agcagcCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCAGAgtgt                                             <  2:1075267/140‑1 (MQ=255)
                                                                                           gcagcCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCAGAGtgtg                                            <  2:279967/140‑1 (MQ=255)
                                                                                                         cGGTTGTTCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCAGAGTGTGCAGCGGTACGCGAc                              <  2:486170/140‑1 (MQ=255)
                                                                                                              gttCAACAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCAGAGTGTGCAGCGGTACGCGACCTTcg                         >  1:1111913/1‑140 (MQ=255)
                                                                                                                   aCAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCAGAGTGTGCAGCGGTACGCGACCTTCGCGGTa                    <  2:1185136/140‑1 (MQ=255)
                                                                                                                   aCAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCAGAGTGTGCAGCGGTACGCGACCTTCGCGGTa                    <  2:1111913/140‑1 (MQ=255)
                                                                                                                   aCAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCAGAGTGTGCAGCGGTACGCGACCTTCGCGGTa                    <  1:78263/140‑1 (MQ=255)
                                                                                                                   aCAGCAGCCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGCGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCAGAGTGTGCAGCGGTACGCGACCTTCGCGGTa                    <  1:341219/140‑1 (MQ=255)
                                                                                                                      gcagcCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCAGAGTGTGCAGCGGTACGCGACCTTCGCGGTACCa                 >  2:906799/1‑140 (MQ=255)
                                                                                                                         gcCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCAGAGTGTGCAGCGGTACGCGACCTTCGCGGTACCATTc              >  2:536019/1‑140 (MQ=255)
                                                                                                                         gcCATACCACCCAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCAGAGTGTGCAGCGGTACGCGACCTTCGCGGTACCATTc              >  1:484609/1‑140 (MQ=255)
                                                                                                                                   ccAGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCAGAGTGTGCAGCGGTACGCGACCTTCGCGGCACCATTCGGTACGTGCg    >  1:1011160/1‑140 (MQ=255)
                                                                                                                                     aGGTTCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCAGAGTGTGCAGCGGTACGCGACCTTCGCGGTACCATTCGGTACGTGCGAt  >  2:769913/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACAGCAGCCATACCACCCAGGATCTCGCCTTTGAAGATCCACACTTTAACGCCGATTACACCGTAAGTGGTGTGCGCTTCAGAGGTGTTGTAGTCGATGTCAGCACGCAGAGTGTGCAGCGGTACGCGACCTTCGCGGTACCATTCGGTACGTGCGAT  >  NC_000913/3449128‑3449400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A