Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	224	87.5	2960670	99.3%	2939945	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,451,262	C→T	E35K (GAA→AAA)	rplB ←	50S ribosomal subunit protein L2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,451,262	0	C	T	98.8%	275.2 / ‑5.4	80	E35K (GAA→AAA)	rplB	50S ribosomal subunit protein L2
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (37/42); total (37/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GTTCAACAACTGCCGGGATACCGTCTTTGTTGCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTCCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACG  >  NC_000913/3451127‑3451400

gTTCAACAACTGCCGGGATACCGTCTTTGTTGCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGc                                                                                                                                        <  1:377424/140‑1 (MQ=255)

gTTCAACAACTGCCGGGATACCGTCTTTGTTGCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGc                                                                                                                                        >  1:1079233/1‑140 (MQ=255)

gTTCAACAACTGCCGGGATACCGTCTTTGTTGCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGc                                                                                                                                        >  1:1132983/1‑140 (MQ=255)

gTTCAACAACTGCCGGGATACCGTCTTTGTTGCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGc                                                                                                                                        >  1:339421/1‑140 (MQ=255)

     acaacTGCCGGGATACCGTCTTTGTTGCGTTTGAAGTCAACAATACGGTAAGCTTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACgg                                                                                                                                   >  2:1034299/1‑140 (MQ=255)

        acTGCCGGGATACCGTCTTTGTTGCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAAc                                                                                                                                     <  1:368118/135‑1 (MQ=255)

        acTGCCGGGATACCGTCTTTGTTGCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAAc                                                                                                                                     >  2:368118/1‑135 (MQ=255)

         cTGCCGGGATACCGTCTTTGTTGCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCa                                                                                                                               <  1:133400/140‑1 (MQ=255)

         cTGCCGGGATACCGTCTTTGTTGCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCa                                                                                                                               <  2:1280906/140‑1 (MQ=255)

              gggATACCGTCTTTGTTGCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAgg                                                                                                                          >  2:1339710/1‑140 (MQ=255)

               ggATACCGTCTTTGTTGCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGt                                                                                                                         <  2:887666/140‑1 (MQ=255)

                gATACCGTCTTTGTTGCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGtt                                                                                                                        <  2:969270/140‑1 (MQ=255)

                gATACCGTCTTTGTTGCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGtt                                                                                                                        <  2:120688/140‑1 (MQ=255)

                   aCCGTCTTTGTTGCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGc                                                                                                                     >  2:442638/1‑140 (MQ=255)

                     cGTCTTTGTTGCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGccc                                                                                                                   <  1:442638/140‑1 (MQ=255)

                     cGTCTTTGTTGCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGccc                                                                                                                   <  2:986253/140‑1 (MQ=255)

                         tttGTTGCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTtgt                                                                                                               >  2:1402619/1‑140 (MQ=255)

                          ttgttgCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGtt                                                                                                                        >  2:499935/1‑130 (MQ=255)

                          ttgttgCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGtt                                                                                                                        <  1:499935/130‑1 (MQ=255)

                          ttgttgCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTtgtg                                                                                                              <  1:784001/140‑1 (MQ=255)

                          ttgttgCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTtgtg                                                                                                              <  1:484346/140‑1 (MQ=255)

                               gCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCt                                                                                                         <  2:1079233/140‑1 (MQ=255)

                               gCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCt                                                                                                         >  1:688448/1‑140 (MQ=255)

                               gCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCt                                                                                                         <  2:1038110/140‑1 (MQ=255)

                               gCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCt                                                                                                         <  1:358870/140‑1 (MQ=255)

                                  tttGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAg                                                                                                      >  1:236906/1‑140 (MQ=255)

                                   ttGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAgg                                                                                                     <  2:1132983/140‑1 (MQ=255)

                                    tGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAggg                                                                                                    >  2:525821/1‑140 (MQ=255)

                                                tACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTa                                                                                        >  1:1463518/1‑140 (MQ=255)

                                                 aCGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTaa                                                                                       >  1:442592/1‑140 (MQ=255)

                                                   ggTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAGAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACt                                                                                     >  2:145881/1‑140 (MQ=255)

                                                           tGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGcg                                                                             <  2:804738/140‑1 (MQ=255)

                                                            gCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGcga                                                                            <  2:688448/140‑1 (MQ=255)

                                                                  ggCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGAccc                                                                      >  2:16967/1‑140 (MQ=255)

                                                                  ggCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGAccc                                                                      >  2:168074/1‑140 (MQ=255)

                                                                       ccaccGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGgaga                                                                 >  2:806649/1‑140 (MQ=255)

                                                                        caccGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCg                                                                     <  1:802239/135‑1 (MQ=255)

                                                                        caccGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCg                                                                     >  2:802239/1‑135 (MQ=255)

                                                                             atatGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCgg                                                           >  1:438604/1‑140 (MQ=255)

                                                                              tatGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGt                                                          <  1:1402619/140‑1 (MQ=255)

                                                                              tatGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGt                                                          <  2:417788/140‑1 (MQ=255)

                                                                                  aCGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGt                                                          >  1:665666/1‑136 (MQ=255)

                                                                                  aCGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGt                                                          <  2:665666/136‑1 (MQ=255)

                                                                                  aCGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTAc                                                      >  2:11859/1‑140 (MQ=255)

                                                                                  aCGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTAc                                                      >  1:1037862/1‑140 (MQ=255)

                                                                                     aGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACAtt                                                   >  1:1244662/1‑140 (MQ=255)

                                                                                     aGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACAtt                                                   >  2:1474017/1‑140 (MQ=255)

                                                                                          tgATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTaaca                                              <  1:1272041/140‑1 (MQ=255)

                                                                                              aCGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTg                                          >  1:1003110/1‑140 (MQ=255)

                                                                                               cGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGc                                         <  1:1474017/140‑1 (MQ=255)

                                                                                               cGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGc                                         <  2:236906/140‑1 (MQ=255)

                                                                                               cGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGc                                         <  2:1463518/140‑1 (MQ=255)

                                                                                               cGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGc                                         <  2:1037862/140‑1 (MQ=255)

                                                                                               cGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGc                                         <  2:213868/140‑1 (MQ=255)

                                                                                               cGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGc                                         <  2:227946/140‑1 (MQ=255)

                                                                                                 gCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACTACCCGGAGATGTCGGTTTACATTTAACAACTGCCa                                       <  1:450023/140‑1 (MQ=255)

                                                                                                 gCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGcta                                       <  1:16967/140‑3 (MQ=255)

                                                                                                 gCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCa                                       >  1:7441/1‑140 (MQ=255)

                                                                                                 gCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCa                                       <  2:311722/140‑1 (MQ=255)

                                                                                                 gCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCa                                       <  2:1244662/140‑1 (MQ=255)

                                                                                                 gCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCa                                       <  1:168074/140‑1 (MQ=255)

                                                                                                 gCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCa                                       >  2:1005925/1‑140 (MQ=255)

                                                                                                 gCCATTGTTGTTACGACCACCGGATTCGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCa                                       <  1:1034299/140‑1 (MQ=255)

                                                                                                  ccATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCa                                       >  2:337939/1‑139 (MQ=255)

                                                                                                  ccATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCa                                       <  1:337939/139‑1 (MQ=255)

                                                                                                  ccATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCAt                                      <  1:525821/140‑1 (MQ=255)

                                                                                                    aTTGTTGTTACGACCACCGGGTTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCa                                       >  2:601949/1‑137 (MQ=255)

                                                                                                    aTTGTTGTTACGACCACCGGGTTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCa                                       <  1:601949/137‑1 (MQ=255)

                                                                                                    aTTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTg                                    >  2:906025/1‑140 (MQ=255)

                                                                                                    aTTGTTGTTACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTg                                    >  1:1153030/1‑140 (MQ=255)

                                                                                                        ttgttACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTAtt                                <  1:1339710/140‑1 (MQ=255)

                                                                                                          gttACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTGTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTAc                              >  2:1322212/1‑140 (MQ=255)

                                                                                                           ttACGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTAct                             >  2:265553/1‑140 (MQ=255)

                                                                                                             aCGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTActcc                           >  2:1079491/1‑140 (MQ=255)

                                                                                                              cGACCACCGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTActcct                          <  2:442592/140‑1 (MQ=255)

                                                                                                                   accGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGAc                     >  1:499490/1‑140 (MQ=255)

                                                                                                                   accGGATTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGAc                     <  2:339421/140‑1 (MQ=255)

                                                                                                                        aTTTGCTGTTTTTTTTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGCCCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACt                <  1:806649/140‑1 (MQ=255)

                                                                                                                                 ttttttTCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGcgccgc       >  2:1085432/1‑140 (MQ=255)

                                                                                                                                      tCCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACg  <  2:1003110/140‑1 (MQ=255)

GTTCAACAACTGCCGGGATACCGTCTTTGTTGCGTTTGAAGTCAACAATACGGTAAGCCTGCTTGTGGCCACCACCGATATGACGAGTGGTGATACGGCCATTGTTGTTACGACCACCGGATTTGCTGTTTTTTTCCAGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACG  >  NC_000913/3451127‑3451400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A