Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	162	86.7	2935410	99.3%	2914862	138.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,424,457	(A)_8→9	intergenic (+71/+59)	ulaF → / ← yjfY	L‑ribulose‑5‑phosphate 4‑epimerase UlaF/DUF1471 domain‑containing protein YjfY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,424,449	1	.	A	92.3%	256.7 / 11.5	78	intergenic (+63/+67)	ulaF/yjfY	L‑ribulose‑5‑phosphate 4‑epimerase UlaF/DUF1471 domain‑containing protein YjfY
Reads supporting (aligned to +/- strand): ref base . (4/2); new base A (34/38); total (38/40)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

ATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACG  >  NC_000913/4424310‑4424582

aTCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTc                                                                                                                                         >  2:543873/1‑140 (MQ=255)

aTCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTc                                                                                                                                         >  2:1154636/1‑140 (MQ=255)

aTCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTc                                                                                                                                         >  1:1316998/1‑140 (MQ=255)

      tCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑‑aaaaaa                                                                                                                                 >  2:827547/1‑140 (MQ=255)

      tCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑‑aaaaaa                                                                                                                                 >  2:1356756/1‑140 (MQ=255)

      tCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑‑aaaaaa                                                                                                                                 >  1:1400816/1‑140 (MQ=255)

           aGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑‑aaaaaaaaagc                                                                                                                            <  2:58456/140‑4 (MQ=255)

           aGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑‑aaaaaaaaagc                                                                                                                            <  1:543873/140‑4 (MQ=255)

             cTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑‑aaaaaaaaagc                                                                                                                            <  2:687793/138‑4 (MQ=255)

             cTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑‑aaaaaaaaagc                                                                                                                            >  1:687793/1‑135 (MQ=255)

                       aaTAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAgg                                                                                                                 >  1:1067941/1‑140 (MQ=255)

                       aaTAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAgg                                                                                                                 >  2:595489/1‑140 (MQ=255)

                       aaTAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAgg                                                                                                                 >  2:853571/1‑140 (MQ=255)

                         tAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑‑aaaaaaaaa                                                                                                                              >  1:902084/1‑123 (MQ=255)

                         tAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑‑aaaaaaaaa                                                                                                                              <  2:902084/124‑2 (MQ=255)

                          aaaCACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAggggg                                                                                                              <  2:1007618/140‑1 (MQ=255)

                          aaaCACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAggggg                                                                                                              <  2:1117492/140‑1 (MQ=255)

                          aaaCACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAggggg                                                                                                              <  1:827547/140‑1 (MQ=255)

                                ttCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGAAAATTTCCTTCCTCTGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGgcaaag                                                                                                         <  1:1286435/139‑1 (MQ=255)

                                ttCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGAAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGgcaaag                                                                                                         >  2:1286435/1‑139 (MQ=255)

                                 tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑‑AAAAAAAAGCCCCCTGCGAAGGGGgcaaagcaa                                                                                                      >  1:996358/1‑140 (MQ=255)

                                      cGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTa                                                                                                  >  1:404752/1‑140 (MQ=255)

                                       gTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCTGCCGTTTTTCAAAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTa                                                                                                  <  1:40040/140‑1 (MQ=255)

                                        tAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATg                                                                                                >  2:971419/1‑140 (MQ=255)

                                          aaCACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGc                                                                                              >  1:1183639/1‑140 (MQ=255)

                                          aaCACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGc                                                                                              >  2:475064/1‑140 (MQ=255)

                                           acacGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCa                                                                                             <  1:360180/140‑1 (MQ=255)

                                            cacGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCaa                                                                                            <  1:971419/140‑1 (MQ=255)

                                             acGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAAt                                                                                           <  1:1154636/140‑1 (MQ=255)

                                                gTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGtt                                                                                        >  1:925415/1‑140 (MQ=255)

                                                   cTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCg                                                                                     <  1:595489/140‑1 (MQ=255)

                                                   cTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCg                                                                                     <  2:1400816/140‑1 (MQ=255)

                                                   cTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCg                                                                                     <  2:1258647/140‑1 (MQ=255)

                                                   cTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCg                                                                                     <  1:1237455/140‑1 (MQ=255)

                                                      aCGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTg                                                                                  <  1:594641/140‑1 (MQ=255)

                                                      aCGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑‑AAAAAAAAAGCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTgg                                                                                 >  2:939270/1‑140 (MQ=255)

                                                        gCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGt                                                                                <  1:853571/140‑1 (MQ=255)

                                                         cTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGtt                                                                               <  2:1296640/140‑1 (MQ=255)

                                                           tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTc                                                                                      >  2:849744/1‑131 (MQ=255)

                                                           tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTc                                                                                      <  1:849744/131‑1 (MQ=255)

                                                           tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCg                                                                                     >  2:1174825/1‑132 (MQ=255)

                                                           tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCg                                                                                     <  2:1243005/132‑1 (MQ=255)

                                                           tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCg                                                                                     >  1:1243005/1‑132 (MQ=255)

                                                           tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCg                                                                                     <  1:1174825/132‑1 (MQ=255)

                                                           tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtat                                                                             >  2:452277/1‑140 (MQ=255)

                                                           tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtat                                                                             >  1:1026829/1‑140 (MQ=255)

                                                                   gCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGt                                                                                    >  2:1323928/1‑125 (MQ=255)

                                                                   gCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGt                                                                                    <  1:1323928/125‑1 (MQ=255)

                                                                     aGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCt                                                                   <  1:1175517/140‑1 (MQ=255)

                                                                        aGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCg                                                               <  2:996358/140‑1 (MQ=255)

                                                                             aaCACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATaa                                                           >  1:1150271/1‑140 (MQ=255)

                                                                                 cgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAatat                                                       >  2:1012789/1‑140 (MQ=255)

                                                                                 cgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAatat                                                       >  2:612865/1‑140 (MQ=255)

                                                                                 cgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAatat                                                       >  2:605889/1‑140 (MQ=255)

                                                                                 cgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAatat                                                       >  2:58549/1‑140 (MQ=255)

                                                                                 cgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAatat                                                       >  2:365097/1‑140 (MQ=255)

                                                                                 cgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑‑AAAAAAAAAGCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATc                                                      >  2:629668/1‑140 (MQ=255)

                                                                                  gcgcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATc                                                      >  1:1329808/1‑140 (MQ=255)

                                                                                   cgcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATcc                                                     >  1:312384/1‑140 (MQ=255)

                                                                                     cTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCcgc                                                   >  1:315700/1‑140 (MQ=255)

                                                                                      tGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCGGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATGCCTGGTGCTAGCGATAAATATCcgcg                                                  <  1:121223/140‑1 (MQ=255)

                                                                                      tGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCcgcg                                                  <  2:315700/140‑1 (MQ=255)

                                                                                      tGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCcgcg                                                  >  2:594715/1‑140 (MQ=255)

                                                                                        cGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCt                                                <  1:612865/140‑1 (MQ=255)

                                                                                            aaTTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCg                                            <  2:682024/140‑1 (MQ=255)

                                                                                                   ttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCatat                                     <  2:827814/140‑1 (MQ=255)

                                                                                                            agaTAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTAc                            <  2:925415/140‑1 (MQ=255)

                                                                                                               tAACTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTCATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTcc                         <  2:1183639/140‑1 (MQ=255)

                                                                                                                aattGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTccc                        >  2:1180172/4‑140 (MQ=255)

                                                                                                                aaCTGGTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTccc                        >  1:977042/1‑140 (MQ=255)

                                                                                                                     gTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCg                                >  2:902457/1‑127 (MQ=255)

                                                                                                                     gTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCg                                <  1:902457/127‑1 (MQ=255)

                                                                                                                     gTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGtt                   >  2:344508/1‑140 (MQ=255)

                                                                                                                     gTCTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGtt                   >  2:758783/1‑140 (MQ=255)

                                                                                                                       cTAATTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTcc                 <  1:452277/140‑1 (MQ=255)

                                                                                                                          aTTCCGCAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGc              <  2:643139/140‑1 (MQ=255)

                                                                                                                                cAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGa        >  2:1229428/1‑140 (MQ=255)

                                                                                                                                cAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGa        >  1:167394/1‑140 (MQ=255)

                                                                                                                                cAGCCGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGa        >  1:409746/1‑140 (MQ=255)

                                                                                                                                cAGCCGTTTTTC‑‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGt                    <  1:20580/127‑1 (MQ=255)

                                                                                                                                cAGCCGTTTTTC‑‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGt                    >  2:20580/1‑127 (MQ=255)

                                                                                                                                   ccGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAAtt     <  1:1229428/140‑1 (MQ=255)

                                                                                                                                    cGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa    <  2:834750/140‑1 (MQ=255)

                                                                                                                                    cGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa    <  1:605889/140‑1 (MQ=255)

                                                                                                                                    cGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa    >  1:821632/1‑140 (MQ=255)

                                                                                                                                    cGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa    <  2:977042/140‑1 (MQ=255)

                                                                                                                                    cGTTTTTCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa    <  1:1012789/140‑1 (MQ=255)

                                                                                                                                      tttttCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg  <  1:1356756/140‑1 (MQ=255)

                                                                                                                                      tttttCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg  <  1:497632/140‑1 (MQ=255)

                                                                                                                                      tttttCA‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg  <  2:1320413/140‑1 (MQ=255)

ATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACG  >  NC_000913/4424310‑4424582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A