Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	121	68.4	2351762	99.3%	2335299	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	684,726	T→C	D177G (GAC→GGC)	gltL ←	glutamate/aspartate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	684,726	0	T	C	100.0%	188.7 / NA	52	D177G (GAC→GGC)	gltL	glutamate/aspartate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (29/23); total (29/23)

AGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGA  >  NC_000913/684590‑684857

aGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCa                                                                                                                                  >  2:688168/1‑140 (MQ=255)

  cGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGt                                                                                                                                >  1:211757/1‑140 (MQ=255)

  cGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGt                                                                                                                                >  2:630888/1‑140 (MQ=255)

     cTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACt                                                                                                                             >  2:677984/1‑140 (MQ=255)

        tCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCg                                                                                                                          <  1:131255/140‑1 (MQ=255)

            cGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGa                                                                                                                      <  1:688168/140‑1 (MQ=255)

                          ttttACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATcc                                                                                                        >  2:436740/1‑140 (MQ=255)

                          ttttACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATcc                                                                                                        >  2:780617/1‑140 (MQ=255)

                                    gTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATg                                                                                              >  2:749690/1‑140 (MQ=255)

                                    gTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATg                                                                                              >  2:598678/1‑140 (MQ=255)

                                     tCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGt                                                                                             >  1:1105562/1‑140 (MQ=255)

                                       cATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCg                                                                                           <  2:1124166/140‑1 (MQ=255)

                                          aaaGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGtt                                                                                        <  1:749690/140‑1 (MQ=255)

                                              aTCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTc                                                                                    >  1:379440/1‑140 (MQ=255)

                                                 aCCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCaaa                                                                                 >  2:229065/1‑140 (MQ=255)

                                                 aCCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCaaa                                                                                 >  2:447167/1‑140 (MQ=255)

                                                 aCCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCaaa                                                                                 >  1:258340/1‑140 (MQ=255)

                                                 aCCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCaaa                                                                                 >  1:877985/1‑140 (MQ=255)

                                                  cccGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAAc                                                                                >  2:779267/1‑140 (MQ=255)

                                                      aTTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCa                                                                            <  1:598678/140‑1 (MQ=255)

                                                       ttCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCAt                                                                           <  1:891416/140‑1 (MQ=255)

                                                        tCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATc                                                                          >  2:909669/1‑140 (MQ=255)

                                                            cACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCaa                                                                      <  2:1105562/140‑1 (MQ=255)

                                                             aCTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAAt                                                                     <  2:1063717/140‑1 (MQ=255)

                                                             aCTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAAt                                                                     <  1:447167/140‑1 (MQ=255)

                                                             aCTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAAt                                                                     <  1:630888/140‑1 (MQ=255)

                                                             aCTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAAt                                                                     <  2:340275/140‑1 (MQ=255)

                                                               tttACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAg                                                                   <  1:780617/140‑1 (MQ=255)

                                                               tttACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAg                                                                   <  1:256296/140‑1 (MQ=255)

                                                               tttACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAg                                                                   <  1:335605/140‑1 (MQ=255)

                                                                      gCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAg                                                                   >  1:696363/1‑133 (MQ=255)

                                                                      gCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAg                                                                   <  2:696363/133‑1 (MQ=255)

                                                                          aGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATacac                                                        >  2:189056/1‑140 (MQ=255)

                                                                            cccATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAGCAGCATCGCAATAGGATCCATACACaa                                                      <  2:814599/140‑1 (MQ=255)

                                                                                tttCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAg                                                                   >  2:24289/1‑123 (MQ=255)

                                                                                tttCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAg                                                                   <  1:24289/123‑1 (MQ=255)

                                                                                   cGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCgcga                                               <  2:877985/140‑1 (MQ=255)

                                                                                       ggTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCgcgagcga                                           <  2:379440/140‑1 (MQ=255)

                                                                                          caccacCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTg                                        <  1:982280/140‑1 (MQ=255)

                                                                                           accaccATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGc                                       >  1:418863/1‑140 (MQ=255)

                                                                                                  tcatGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACgc                                >  2:1061671/1‑140 (MQ=255)

                                                                                                  tcatGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACgc                                >  2:72212/1‑140 (MQ=255)

                                                                                                       gTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACgctgctg                           >  2:379955/1‑140 (MQ=255)

                                                                                                         cATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACgctgctgct                         >  1:762204/1‑140 (MQ=255)

                                                                                                          aTTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGATTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGctgctgctg                        >  2:1015104/1‑140 (MQ=255)

                                                                                                            tCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGAc                      <  1:1166794/140‑1 (MQ=255)

                                                                                                            tCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGAc                      >  2:1166794/1‑140 (MQ=255)

                                                                                                              cTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGAccg                    >  2:664281/1‑140 (MQ=255)

                                                                                                               ttcgttcgCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGAccgc                   <  2:211757/140‑1 (MQ=255)

                                                                                                                tcgttcgCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGAccgcc                  >  1:95428/1‑140 (MQ=255)

                                                                                                                               accaTCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCgg   <  2:95428/140‑1 (MQ=255)

                                                                                                                                ccaTCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGa  >  1:306870/1‑140 (MQ=255)

AGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGA  >  NC_000913/684590‑684857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A