Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	151	87.4	2971636	99.2%	2947862	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,462,889	(C)_9→10	intergenic (+243/‑123)	fadL → / → yfdF	long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,462,880	1	.	C	88.7%	187.6 / 17.4	62	intergenic (+234/‑132)	fadL/yfdF	long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
Reads supporting (aligned to +/- strand): ref base . (4/3); new base C (23/32); total (27/35)
Fisher's exact test for biased strand distribution p-value = 6.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.27e-01

TTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CT‑‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCT  >  NC_000913/2462741‑2463016

ttttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑Ct                                                                                                                                            <  2:197636/140‑1 (MQ=255)

    gCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CT‑‑cccc                                                                                                                                      <  2:1427219/140‑1 (MQ=255)

    gCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CT‑‑cccc                                                                                                                                      <  1:1333397/140‑1 (MQ=255)

       aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CT‑‑ccccccc                                                                                                                                   <  1:496487/140‑1 (MQ=255)

        aCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CT‑‑cccccccc                                                                                                                                  <  1:971424/140‑1 (MQ=255)

        aCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CT‑‑cccccccc                                                                                                                                  <  1:385437/140‑1 (MQ=255)

        aCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CT‑‑cccccccc                                                                                                                                  <  2:651616/140‑1 (MQ=255)

        aCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CT‑‑cccccccc                                                                                                                                  <  2:684684/140‑1 (MQ=255)

        aCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CT‑‑cccccccc                                                                                                                                  <  1:761287/140‑1 (MQ=255)

        aCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CT‑‑cccccccc                                                                                                                                  <  1:114327/140‑1 (MQ=255)

          aGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CT‑‑CCCCCCCCCt                                                                                                                                >  1:552903/1‑140 (MQ=255)

            tcTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CT‑‑cccccccccctg                                                                                                                              >  2:1364514/2‑137 (MQ=255)

                    cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATtgt                                                                                                                       >  2:1091790/1‑140 (MQ=255)

                     aGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATtgtg                                                                                                                      <  2:57785/140‑1 (MQ=255)

                     aGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATtgtg                                                                                                                      <  2:554010/140‑1 (MQ=255)

                     aGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATtgtg                                                                                                                      <  2:808928/140‑1 (MQ=255)

                     aGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATtgtg                                                                                                                      <  1:468983/140‑1 (MQ=255)

                      gCTTTCCCTTTCAAAACAATAAACGGTTTCCACAAGAAAACCCGAAAGGAAAGGCATAATTTCAGGCCTTTTTCTCCTCCTTCTCAAAGAGTTTTCTTTTCTGCATTTCCAGGATACCCC‑‑CCCCCCCCCTGGCTATtgtg                                                                                                                      <  2:309870/140‑1 (MQ=255)

                      gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTCCCCCCCCCCCTGGCTATtgtg                                                                                                                      <  1:617693/140‑1 (MQ=255)

                      gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGACCTTCTTAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTgc                                                                                                                     >  2:249674/1‑140 (MQ=255)

                       cTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTgcg                                                                                                                    <  2:1349683/140‑1 (MQ=255)

                          gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTCCCCCCCCCCCTGGCTATTGTGCGCt                                                                                                                  >  2:20689/1‑140 (MQ=255)

                          gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTa                                                                                                                           >  1:539171/1‑130 (MQ=255)

                          gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTa                                                                                                                           <  2:539171/130‑1 (MQ=255)

                          gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATtgtg                                                                                                                      >  1:693894/1‑135 (MQ=255)

                          gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATtgtg                                                                                                                      <  2:693894/135‑1 (MQ=255)

                           cccTTTCAAATCAATACGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTTTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCa                                                                                                                <  2:1334065/140‑1 (MQ=255)

                           cccTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCa                                                                                                                <  2:740653/140‑1 (MQ=255)

                                tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATacac                                                                                                           <  2:628592/140‑1 (MQ=255)

                                 cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACt                                                                                                          <  2:936119/140‑1 (MQ=255)

                                        aTAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTa                                                                                                   >  2:287146/1‑140 (MQ=255)

                                        aTAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CT‑‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTaa                                                                                                  >  1:693569/1‑140 (MQ=255)

                                         tAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTaa                                                                                                  >  2:174400/1‑140 (MQ=255)

                                            gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGa                                                                                               <  1:890417/140‑1 (MQ=255)

                                            gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CT‑‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                              <  2:552903/140‑1 (MQ=255)

                                             cGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                              <  1:1091790/140‑1 (MQ=255)

                                                tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                              >  2:1221506/1‑137 (MQ=255)

                                                tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                              <  1:1221506/137‑1 (MQ=255)

                                                tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAgg                                                                                           <  1:983176/140‑1 (MQ=255)

                                                 aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGt                                                                                          >  1:238582/1‑140 (MQ=255)

                                                  tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                         >  2:370257/1‑140 (MQ=255)

                                                  tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                         <  1:174400/140‑1 (MQ=255)

                                                    cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCt                                                                                       <  1:1405085/140‑1 (MQ=255)

                                                       aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaa                                                                                    >  1:216343/1‑140 (MQ=255)

                                                       aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaa                                                                                    >  1:1150279/1‑140 (MQ=255)

                                                       aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCta                                                                                      <  2:1277651/138‑1 (MQ=255)

                                                       aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCta                                                                                      >  1:1277651/1‑138 (MQ=255)

                                                         aCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTCCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaat                                                                                   >  2:629655/1‑140 (MQ=255)

                                                             aCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CT‑‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGa                                                                             >  2:416167/1‑140 (MQ=255)

                                                                tGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTCCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                            <  1:20689/140‑1 (MQ=255)

                                                                  aaGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CT‑‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGtt                                                                        <  2:359522/140‑1 (MQ=255)

                                                                         aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                  >  1:742539/1‑140 (MQ=255)

                                                                         aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACCCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                  >  2:337012/1‑140 (MQ=255)

                                                                          ggCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTCCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                  <  1:629655/140‑1 (MQ=255)

                                                                             atatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGt                                                              <  1:337012/140‑1 (MQ=255)

                                                                               atTTTCAGGAGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCt                                                            >  2:201873/1‑140 (MQ=255)

                                                                                ttttCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                           <  1:485961/140‑1 (MQ=255)

                                                                                     aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                      <  1:448447/140‑1 (MQ=255)

                                                                                       gagTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                    <  1:201873/138‑1 (MQ=255)

                                                                                       gCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                    <  2:216343/140‑1 (MQ=255)

                                                                                       gCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                    <  1:777805/140‑1 (MQ=255)

                                                                                        ggTTCTGCTCGTCCTTCTCAAAAAGTTTCCTTTTCTGCCTTTCCAAGATAC‑CC‑‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTa                                                   <  2:799820/139‑1 (MQ=255)

                                                                                         gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CT‑‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                                 <  2:693569/140‑1 (MQ=255)

                                                                                          ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                                 <  2:742539/140‑1 (MQ=255)

                                                                                           tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CT‑‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                                               >  1:3752/1‑140 (MQ=255)

                                                                                            cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                      <  1:1061984/133‑1 (MQ=255)

                                                                                            cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                      >  2:1061984/1‑133 (MQ=255)

                                                                                            cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                                 >  2:615755/1‑138 (MQ=255)

                                                                                            cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                                 <  1:615755/138‑1 (MQ=255)

                                                                                                    ccTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatatatat                                       <  2:283302/140‑1 (MQ=255)

                                                                                                    ccTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatatatat                                       >  1:669781/1‑140 (MQ=255)

                                                                                                       tctcAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTg                                    >  1:615423/1‑140 (MQ=255)

                                                                                                           aaaGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAAc                                >  2:472086/1‑140 (MQ=255)

                                                                                                                   tACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatcaat                        >  2:3009/1‑140 (MQ=255)

                                                                                                                   tACTTTTCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatcaat                        >  2:1212618/1‑140 (MQ=255)

                                                                                                                        ttCTGCATTTCCAGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCaaaa                   <  1:3009/140‑1 (MQ=255)

                                                                                                                              aTTTCCAGGATT‑‑CCCCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGaa              <  2:553298/140‑1 (MQ=255)

                                                                                                                                    aGGATA‑‑CTC‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATc       <  2:238582/140‑1 (MQ=255)

                                                                                                                                        tA‑‑CT‑‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCt  <  1:416167/140‑1 (MQ=255)

TTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑CT‑‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCT  >  NC_000913/2462741‑2463016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A