Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	183	98.6	3427438	96.9%	3321187	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,462,889	(C)_9→10	intergenic (+243/‑123)	fadL → / → yfdF	long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,462,880	1	.	C	85.6%	343.1 / 45.8	111	intergenic (+234/‑132)	fadL/yfdF	long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
Reads supporting (aligned to +/- strand): ref base . (7/9); new base C (40/55); total (47/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

TTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑T‑‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTA  >  NC_000913/2462741‑2463017

  ttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑T‑‑‑cc                                                                                                                                          >  2:785186/1‑140 (MQ=255)

    gCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑T‑‑‑cccc                                                                                                                                        <  2:184039/140‑1 (MQ=255)

    gCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑T‑‑‑cccc                                                                                                                                        <  1:366214/140‑1 (MQ=255)

    gCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑T‑‑‑cccc                                                                                                                                        <  1:260768/140‑1 (MQ=255)

     cAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑T‑‑‑ccccc                                                                                                                                       >  2:1063335/1‑140 (MQ=255)

     cAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑T‑‑‑ccccc                                                                                                                                       >  2:1343979/1‑140 (MQ=255)

            ccTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑T‑‑‑cccc                                                                                                                                        <  1:289243/132‑1 (MQ=255)

              tGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑T‑‑‑ccccccccccctgg                                                                                                                              <  1:333020/140‑6 (MQ=255)

                ttGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTa                                                                                                                             >  2:1001729/1‑140 (MQ=255)

                     aGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑Ttgtg                                                                                                                       <  2:915321/140‑1 (MQ=255)

                      gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTTCTTTTCTGCATTTCCAGGATT‑C‑TCC‑CCCCCCCCCTGGCTA‑Ttgtg                                                                                                                       <  1:1367156/140‑1 (MQ=255)

                       cTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTTAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTgcg                                                                                                                     >  1:1606170/1‑140 (MQ=255)

                       cTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TCC‑CCCCCCCCCTGGCTA‑Ttgtg                                                                                                                       >  2:1259128/1‑139 (MQ=255)

                       cTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTgcg                                                                                                                     >  2:298470/1‑140 (MQ=255)

                       cTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTgcg                                                                                                                     <  1:10619/140‑1 (MQ=255)

                       cTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCATTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TCC‑CCCCCCCCCTGGCTA‑Ttgtg                                                                                                                       <  1:1259128/139‑1 (MQ=255)

                        ttGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TCC‑CCCCCCCCCTGGCTA‑TTGTgcg                                                                                                                     <  1:55807/140‑1 (MQ=255)

                        ttGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGGTA‑Atgtggcc                                                                                                                    >  2:1482899/1‑137 (MQ=255)

                        ttGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑T‑‑‑CCCCCCCCCTGGCTA‑TTGTGCGCt                                                                                                                   >  2:1601301/1‑140 (MQ=255)

                          gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTc                                                                                                                  <  1:343766/140‑1 (MQ=255)

                          gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTc                                                                                                                  >  1:1454971/1‑140 (MQ=255)

                          gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTc                                                                                                                  >  2:871556/1‑140 (MQ=255)

                          gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑T‑‑‑ccccccccc                                                                                                                                   >  2:1381233/1‑123 (MQ=255)

                                tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATacac                                                                                                            <  1:871556/140‑1 (MQ=255)

                                  aaaTCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTc                                                                                                          <  1:271726/140‑1 (MQ=255)

                                         tAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TCC‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTa                                                                                                    >  1:297797/1‑140 (MQ=255)

                                            gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑T‑‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGAt                                                                                               <  2:729596/140‑1 (MQ=255)

                                             gggTATCCCCAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGAt                                                                                               <  2:1588416/139‑1 (MQ=255)

                                             cGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGAt                                                                                               <  2:1454971/140‑1 (MQ=255)

                                             cGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGAt                                                                                               <  1:793920/140‑1 (MQ=255)

                                             cGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGAt                                                                                               <  1:247487/140‑1 (MQ=255)

                                              ggTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TCC‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGAt                                                                                               <  2:297797/140‑1 (MQ=255)

                                              ggTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGGCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TCC‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGAt                                                                                               <  2:1288571/140‑1 (MQ=255)

                                                tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TCC‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAg                                                                                             >  2:673196/1‑140 (MQ=255)

                                                tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAgg                                                                                            >  2:733403/1‑140 (MQ=255)

                                                 aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGAt                                                                                               <  2:782388/136‑1 (MQ=255)

                                                 aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGAt                                                                                               >  1:662665/1‑136 (MQ=255)

                                                 aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGAt                                                                                               >  1:782388/1‑136 (MQ=255)

                                                 aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGt                                                                                           >  1:219595/1‑140 (MQ=255)

                                                 aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGt                                                                                           >  2:704061/1‑140 (MQ=255)

                                                  tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                          <  2:966988/140‑1 (MQ=255)

                                                   ccACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TCC‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                          <  1:191358/140‑1 (MQ=255)

                                                   ccACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTc                                                                                         <  1:1402909/140‑1 (MQ=255)

                                                       aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TCCCCCCCCCCCCGGGCTT‑TTGGGCGCTCATACACACAATTTAAAAATAGTTTCta                                                                                       >  1:650889/1‑140 (MQ=255)

                                                       aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaa                                                                                     >  1:1526515/1‑140 (MQ=255)

                                                        gACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGAt                                                                                               <  2:1449223/129‑1 (MQ=255)

                                                        gACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGAt                                                                                               >  1:1449223/1‑129 (MQ=255)

                                                         aCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGAt                                                                                               >  2:1211414/1‑128 (MQ=255)

                                                         aCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGAt                                                                                               <  1:1211414/128‑1 (MQ=255)

                                                            aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTaaataaat                                                                                >  2:1125796/1‑140 (MQ=255)

                                                             aCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑T‑‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGa                                                                              >  1:330423/1‑140 (MQ=255)

                                                             aCCTGAAGGGAAAGGCATATTTTAAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATg                                                                               >  1:739624/1‑140 (MQ=255)

                                                              ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTCTTCAATTTCCAGGATC‑C‑T‑‑‑CCCCCCCCCCTGCTATTTGTCCCCCCTTA‑ACTCAAATTAAAAAATAGTTCTAAATAAATTAg                                                                             >  2:895243/1‑140 (MQ=255)

                                                               cTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                             <  1:1098627/140‑1 (MQ=255)

                                                               cTGAAGGGAAAGGCAAATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACC‑C‑‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                             <  1:1052207/140‑1 (MQ=255)

                                                                 gAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCg                                                                           <  1:785186/140‑1 (MQ=255)

                                                                  aaGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGt                                                                          >  1:1445565/1‑140 (MQ=255)

                                                                   aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                             >  2:1075606/1‑136 (MQ=255)

                                                                   aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                             <  1:1075606/136‑1 (MQ=255)

                                                                     ggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TCC‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGttt                                                                        >  1:720836/1‑140 (MQ=255)

                                                                       aaaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑T‑‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTg                                                                    <  2:1672428/140‑1 (MQ=255)

                                                                        aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTg                                                                    >  1:136935/1‑140 (MQ=255)

                                                                         aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑T‑‑‑‑CCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa                                                                 >  1:1218336/1‑140 (MQ=255)

                                                                          ggCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TCC‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                   <  2:598889/140‑1 (MQ=255)

                                                                          ggCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TCC‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                   <  2:685180/140‑1 (MQ=255)

                                                                          ggCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TCC‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                   <  2:720836/140‑1 (MQ=255)

                                                                           gCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTACGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa                                                                 <  2:1194155/140‑1 (MQ=255)

                                                                                ttttCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                            <  1:1354303/140‑1 (MQ=255)

                                                                                ttttCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                            <  2:355854/140‑1 (MQ=255)

                                                                                   tCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTAttt                                                         <  1:1001729/140‑1 (MQ=255)

                                                                                    cAGGCGTTCTGTCCGCCCTTCTAAAAGAG‑TTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                       <  1:895243/140‑1 (MQ=255)

                                                                                     aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                       >  2:1085588/1‑140 (MQ=255)

                                                                                     aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                       <  1:733403/140‑1 (MQ=255)

                                                                                     aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                       <  2:558010/140‑1 (MQ=255)

                                                                                     aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑T‑‑‑CCCCCCCCGTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa                                                                 <  2:1503598/129‑1 (MQ=255)

                                                                                       gCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                     <  2:1575782/140‑1 (MQ=255)

                                                                                       gCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                     <  1:1460741/140‑1 (MQ=255)

                                                                                         gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCCTACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAg                                                   <  2:1526515/140‑1 (MQ=255)

                                                                                          ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                   <  1:1413073/123‑1 (MQ=255)

                                                                                          ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                                  >  1:165920/1‑140 (MQ=255)

                                                                                          ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                                  <  1:1085588/140‑1 (MQ=255)

                                                                                          ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                                  >  2:755904/1‑140 (MQ=255)

                                                                                          ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑T‑‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                     <  2:65743/136‑1 (MQ=255)

                                                                                           tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGt                                                 >  1:1488336/1‑140 (MQ=255)

                                                                                                tCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACC‑C‑‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatat                                            <  1:158276/140‑1 (MQ=255)

                                                                                                tCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatat                                            <  1:1125796/140‑1 (MQ=255)

                                                                                                       tctcAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatata                                           <  2:620616/134‑1 (MQ=255)

                                                                                                       tctcAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatata                                           >  1:620616/1‑134 (MQ=255)

                                                                                                         tcAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTa                                   >  1:1520331/1‑140 (MQ=255)

                                                                                                             agagTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACaa                               <  1:1381538/140‑1 (MQ=255)

                                                                                                             agagTTTACTTTTCTGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCAAACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACaa                               >  1:1680462/1‑140 (MQ=255)

                                                                                                                  ttACTTTTCTGCATTTCCAGGATA‑C‑TCC‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatca                           >  1:968500/1‑140 (MQ=255)

                                                                                                                           tGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATgg                 >  2:1055120/1‑140 (MQ=255)

                                                                                                                           tGCATTTCCAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATgg                 >  2:738124/1‑140 (MQ=255)

                                                                                                                             cATTTCCAGGATA‑CAC‑‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTGATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGaa               <  1:1310713/140‑1 (MQ=255)

                                                                                                                                  ccAGGATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATaaaa          >  2:314776/1‑140 (MQ=255)

                                                                                                                                  ccAGGATA‑C‑T‑‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAAt         <  1:1521551/140‑1 (MQ=255)

                                                                                                                                     ggATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCa       <  2:226089/140‑1 (MQ=255)

                                                                                                                                     ggATA‑C‑TC‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCa       <  1:1055120/140‑1 (MQ=255)

                                                                                                                                         a‑C‑T‑‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAGATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTa  <  1:1601301/140‑1 (MQ=255)

TTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑C‑T‑‑‑CCCCCCCCCTGGCTA‑TTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTA  >  NC_000913/2462741‑2463017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A