Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	159	82.1	2830106	99.3%	2810295	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,462,889	(C)_9→10	intergenic (+243/‑123)	fadL → / → yfdF	long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,462,880	1	.	C	84.0%	224.8 / 34.8	75	intergenic (+234/‑132)	fadL/yfdF	long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
Reads supporting (aligned to +/- strand): ref base . (5/7); new base C (24/39); total (29/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

TTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CT‑‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATG  >  NC_000913/2462743‑2463014

ttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CT‑‑cc                                                                                                                                        >  1:1020270/1‑140 (MQ=255)

  gCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATTAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CT‑‑cccc                                                                                                                                      <  2:95629/140‑1 (MQ=255)

  gCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CT‑‑cccc                                                                                                                                      <  2:916552/140‑1 (MQ=255)

  gCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CT‑‑cccc                                                                                                                                      <  1:571085/140‑1 (MQ=255)

   cAAACAGCCTGTTGGCAGCTTGCCCTTTCAGATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CT‑‑cccc‑c                                                                                                                                    <  1:642596/140‑1 (MQ=255)

      aCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CT‑‑cccc‑cccc                                                                                                                                 <  2:765689/140‑1 (MQ=255)

      aCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CT‑‑cccc‑cccc                                                                                                                                 <  1:543894/140‑1 (MQ=255)

      aCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CT‑‑cccc‑cccc                                                                                                                                 <  1:536972/140‑1 (MQ=255)

       cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CT‑‑cccc‑ccccc                                                                                                                                <  1:1083181/140‑1 (MQ=255)

        ggCCTGTTGGCGGCTTGCCCATTCAAACCAATAAACGGTATCCACCGACAAACCGGAAGGGAAAGGCTTATTTTCGGGGGTTCTGCGCGGCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CT‑‑cccc‑cccccc                                                                                                                               <  2:335951/139‑2 (MQ=255)

        aGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CT‑‑cccc‑cccccc                                                                                                                               >  2:181286/1‑139 (MQ=255)

           cTGGTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGGAGGGAAAGGCATATTTTCAGGCGTTTTGCTCCTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGAATA‑Cc‑‑cccc‑ccccccagg                                                                                                                            <  2:256781/140‑5 (MQ=255)

                ggCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CT‑‑CCCC‑CCCCCTTGGCTAtt                                                                                                                       <  1:655848/140‑1 (MQ=255)

                 gCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATtg                                                                                                                      <  2:289734/140‑1 (MQ=255)

                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CT‑‑CCCC‑CCCCC‑TGGCTATtgtg                                                                                                                    <  2:135481/140‑1 (MQ=255)

                   aggttGCCCTTTCAAAACAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGAATATTTTCAGGCGTTCTGCTCGTCCTTCCCAAAGAGTTTACTTTTCTGCATTTCCAGGATG‑CTC‑CCCC‑CCCCC‑TGGCTATtgtg                                                                                                                    <  1:362091/137‑1 (MQ=255)

                   aGCTTGCCCTTTCAAATCAATAAGCGGTATCCACGGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATtgtg                                                                                                                    <  1:1257631/140‑1 (MQ=255)

                   aGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATtgtg                                                                                                                    <  2:1018084/140‑1 (MQ=255)

                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTgc                                                                                                                   >  2:1353112/1‑140 (MQ=255)

                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CT‑‑CCCC‑CCCCC‑TGGCTATTGTgcg                                                                                                                  <  1:144705/140‑1 (MQ=255)

                     cTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTgcg                                                                                                                  <  1:404254/140‑1 (MQ=255)

                        gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATtgtg                                                                                                                    >  1:245928/1‑135 (MQ=255)

                        gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATtgtg                                                                                                                    <  2:245928/135‑1 (MQ=255)

                        gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTc                                                                                                               >  2:494470/1‑140 (MQ=255)

                        gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTc                                                                                                               >  2:735089/1‑140 (MQ=255)

                              tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTTCTTTTCTGCATTTCCAGGATT‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATacac                                                                                                         <  1:1273111/140‑1 (MQ=255)

                               cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACt                                                                                                        >  2:387200/1‑140 (MQ=255)

                                      aTAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTa                                                                                                 >  1:901449/1‑140 (MQ=255)

                                      aTAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTa                                                                                                 >  2:90794/1‑140 (MQ=255)

                                           cGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  2:1666/140‑1 (MQ=255)

                                           cGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  2:289270/140‑1 (MQ=255)

                                           cGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  1:1010233/140‑1 (MQ=255)

                                           cGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  2:787022/140‑1 (MQ=255)

                                           cGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  1:305789/140‑1 (MQ=255)

                                             gTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAg                                                                                          >  2:1024954/1‑140 (MQ=255)

                                                     aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CT‑‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGGTTtaaaa                                                                                 >  2:320642/1‑139 (MQ=255)

                                                     aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTGTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaa                                                                                  >  2:1244195/1‑140 (MQ=255)

                                                      gACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaat                                                                                 >  1:539829/1‑140 (MQ=255)

                                                      gACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CT‑‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaata                                                                                >  1:1009425/1‑140 (MQ=255)

                                                          aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTTTTGGGGGCCCTAACACTAAAATAAAAATTAGGTTTtaaaaaaag                                                                             >  1:734102/1‑139 (MQ=255)

                                                          aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTaaataaat                                                                             >  2:8251/1‑140 (MQ=255)

                                                           aCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATg                                                                            >  2:1362399/1‑140 (MQ=255)

                                                           aCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATg                                                                            >  1:1163189/1‑140 (MQ=255)

                                                               aaaGGGAAAGGCATATTTTCAGGCATTGTGCCCCTCCTTCCCAAAGAATTTACTTCTGTGCATTTCCAGGATACCC‑‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCGAATTAAAGATAGGTTCTAAATAAATGAGCg                                                                        <  2:607926/139‑1 (MQ=255)

                                                                  gggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGttt                                                                     <  2:1020270/140‑1 (MQ=255)

                                                                     aaaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGTTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGAGtttttt                                                                  >  2:2519/1‑140 (MQ=255)

                                                                       aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                <  2:525871/140‑1 (MQ=255)

                                                                       aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                <  1:387200/140‑1 (MQ=255)

                                                                       aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                <  2:34751/140‑1 (MQ=255)

                                                                       aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                <  2:1165455/140‑1 (MQ=255)

                                                                       aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CT‑‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGAt                                                               >  1:60188/1‑140 (MQ=255)

                                                                         gCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa                                                              <  2:1220932/140‑1 (MQ=255)

                                                                               tttCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTCCCCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                         <  2:9048/140‑1 (MQ=255)

                                                                                  cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CT‑‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                    <  1:1046890/140‑1 (MQ=255)

                                                                                   aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                    <  1:1362399/140‑1 (MQ=255)

                                                                                   aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                    <  2:330697/140‑1 (MQ=255)

                                                                                   aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                    <  1:1079642/140‑1 (MQ=255)

                                                                                   aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                    <  1:645240/140‑1 (MQ=255)

                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt                                                   >  1:1332828/1‑140 (MQ=255)

                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt                                                   <  1:968810/140‑1 (MQ=255)

                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CC‑‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                  <  1:320642/140‑1 (MQ=255)

                                                                                     gCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                  <  1:494470/140‑1 (MQ=255)

                                                                                     gCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                  <  1:1087347/140‑1 (MQ=255)

                                                                                       gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAg                                                <  2:1109352/140‑1 (MQ=255)

                                                                                        ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTa                                                 <  2:721746/138‑1 (MQ=255)

                                                                                        ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTa                                                 >  1:721746/1‑138 (MQ=255)

                                                                                        ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                               <  2:937859/140‑1 (MQ=255)

                                                                                          cTGCTCGTCCTTCTCAAAGAGTTTTCTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg                                                             <  2:1317505/124‑1 (MQ=255)

                                                                                          cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg                                                             >  1:1317505/1‑124 (MQ=255)

                                                                                          cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                                             <  1:132959/140‑1 (MQ=255)

                                                                                          cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                                             >  1:735552/1‑140 (MQ=255)

                                                                                           tGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTaa                                            >  2:886776/1‑140 (MQ=255)

                                                                                              tCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatat                                         <  2:1163189/140‑1 (MQ=255)

                                                                                                           agagTTTACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACaa                            >  1:1125387/1‑140 (MQ=255)

                                                                                                             gttttACTTTTCTGCACTTCCATGATA‑CT‑‑CCCCACCCCC‑TGGCTATTGTGCGCTCATACACTCAAAATAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaat                          <  2:858541/138‑1 (MQ=255)

                                                                                                               tttACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatca                        >  1:1276438/1‑140 (MQ=255)

                                                                                                               tttACTTTTCTGCATTTCCAGGATA‑CT‑‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatcaa                       >  1:818042/1‑140 (MQ=255)

                                                                                                                 tACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatcaat                      <  2:1140635/140‑1 (MQ=255)

                                                                                                                 tACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatcaat                      >  2:1168594/1‑140 (MQ=255)

                                                                                                                 tACTTTTCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatcaat                      <  2:633569/140‑1 (MQ=255)

                                                                                                                     tttCTGCATTTCCAGGATA‑CT‑‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCaaaa                 <  2:60188/140‑1 (MQ=255)

                                                                                                                      ttCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCaaaa                 <  1:997525/140‑1 (MQ=255)

                                                                                                                      ttCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCaaaa                 <  2:395696/140‑1 (MQ=255)

                                                                                                                      ttCTGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCaaaa                 <  2:1322702/140‑1 (MQ=255)

                                                                                                                       tCTGCATTTCCAGGATA‑CT‑‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTa                                <  1:16148/123‑1 (MQ=255)

                                                                                                                       tCTGCATTTCCAGGATA‑CT‑‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTa                                >  2:16148/1‑123 (MQ=255)

                                                                                                                         tGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                       <  2:1113298/67‑1 (MQ=255)

                                                                                                                         tGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                       >  1:1113298/1‑67 (MQ=255)

                                                                                                                         tGCATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATgg              >  1:933582/1‑140 (MQ=255)

                                                                                                                           cATTTCCAGGATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGaa            <  2:137252/140‑1 (MQ=255)

                                                                                                                                   ggATA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCa    <  2:504334/140‑1 (MQ=255)

                                                                                                                                     aTA‑CTC‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATg  <  2:111708/140‑1 (MQ=255)

TTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑CT‑‑CCCC‑CCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATG  >  NC_000913/2462743‑2463014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A