Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,001,304	(G)_7→8	coding (960/2259 nt)	xdhA →	putative xanthine dehydrogenase molybdenum‑binding subunit XdhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,001,297	1	.	G	96.7%	207.6 / ‑0.2	60	coding (953/2259 nt)	xdhA	putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (30/28); total (31/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

CCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑C‑GGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGTTGAGT  >  NC_000913/3001165‑3001431

cccGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑C‑ggggggg                                                                                                                                  >  1:711388/1‑140 (MQ=255)

     aCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑C‑ggggg                                                                                                                                    >  1:1240669/1‑133 (MQ=255)

     aCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑C‑ggggg                                                                                                                                    <  2:1240669/133‑1 (MQ=255)

            aCGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGc                                                                                                                      >  2:384089/1‑140 (MQ=255)

            aCGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGc                                                                                                                      <  1:384089/140‑1 (MQ=255)

             cGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGTCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCt                                                                                                                     <  1:1081977/140‑1 (MQ=255)

             cGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCt                                                                                                                     <  1:517497/140‑1 (MQ=255)

                         tGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCt                                                                                                         >  2:1014139/1‑140 (MQ=255)

                          gACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTc                                                                                                        >  1:1116331/1‑140 (MQ=255)

                            cGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGt                                                                                                      <  2:674897/140‑1 (MQ=255)

                               gCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTtgt                                                                                                   >  2:432733/1‑140 (MQ=255)

                                cAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTTGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTtgtg                                                                                                  <  1:238292/140‑1 (MQ=255)

                                cAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTtgtg                                                                                                  <  1:303009/140‑1 (MQ=255)

                                  aaTGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGcc                                                                                                >  1:301802/1‑140 (MQ=255)

                                   aTGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCt                                                                                               >  2:75011/1‑140 (MQ=255)

                                          tGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTa                                                                                        <  2:204154/140‑1 (MQ=255)

                                           gAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTAc                                                                                       >  2:16877/1‑140 (MQ=255)

                                                 cgACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCa                                                                                 <  1:435527/140‑1 (MQ=255)

                                                 cgACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCa                                                                                 >  1:439922/1‑140 (MQ=255)

                                                    cGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAg                                                                              <  2:579209/140‑1 (MQ=255)

                                                     ggAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGa                                                                             <  1:432733/140‑1 (MQ=255)

                                                     ggAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGa                                                                             <  1:678049/140‑1 (MQ=255)

                                                     ggAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGa                                                                             <  2:568808/140‑1 (MQ=255)

                                                         cATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTg                                                                         <  1:75011/140‑1 (MQ=255)

                                                                   ttATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAAc                                                               <  1:16877/140‑1 (MQ=255)

                                                                        gTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTccc                                                          <  2:711388/140‑1 (MQ=255)

                                                                           tGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTc                                                       >  1:298049/1‑140 (MQ=255)

                                                                            ggATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCg                                                      >  1:726818/1‑140 (MQ=255)

                                                                             gATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCgg                                                     >  2:315770/1‑140 (MQ=255)

                                                                                   cTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTg                                               >  2:602138/1‑140 (MQ=255)

                                                                                   cTGTCTAACACCGGCGCATATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTg                                               <  2:1116331/140‑1 (MQ=255)

                                                                                       cTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGAt                                           >  1:1235160/1‑140 (MQ=255)

                                                                                           cacCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGTG‑GGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGt                                       >  2:291911/1‑140 (MQ=255)

                                                                                                gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTg                                               <  2:732614/127‑1 (MQ=255)

                                                                                                gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTg                                               >  1:732614/1‑127 (MQ=255)

                                                                                                gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCg                                        >  2:636186/1‑134 (MQ=255)

                                                                                                gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCg                                        <  1:636186/134‑1 (MQ=255)

                                                                                                gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTa                                  >  1:9911/1‑140 (MQ=255)

                                                                                                gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTa                                  >  2:1098043/1‑140 (MQ=255)

                                                                                                gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTa                                  >  1:407923/1‑140 (MQ=255)

                                                                                                gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTa                                  >  1:379263/1‑140 (MQ=255)

                                                                                                gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTa                                  >  2:288058/1‑140 (MQ=255)

                                                                                                     tATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGgcg                             <  2:9911/140‑1 (MQ=255)

                                                                                                     tATGCATCTCACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGgcg                             >  1:998924/1‑140 (MQ=255)

                                                                                                           tctcACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCcac                       <  1:957982/140‑1 (MQ=255)

                                                                                                           tctcACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCcac                       >  1:187027/1‑140 (MQ=255)

                                                                                                           tctcACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCcac                       <  1:530845/140‑1 (MQ=255)

                                                                                                              cACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAg                    <  2:407923/140‑1 (MQ=255)

                                                                                                              cACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAg                    >  1:541097/1‑140 (MQ=255)

                                                                                                              cACGGGCACTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAg                    >  1:524112/1‑140 (MQ=255)

                                                                                                                      cTCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTAttt            >  1:571737/1‑140 (MQ=255)

                                                                                                                       tCCATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTg           <  2:439922/140‑1 (MQ=255)

                                                                                                                         cATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGcc         >  1:950201/1‑140 (MQ=255)

                                                                                                                         cATCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGcc         >  2:667036/1‑140 (MQ=255)

                                                                                                                          aTCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCg        <  2:571737/140‑1 (MQ=255)

                                                                                                                           tCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGt       <  1:315770/140‑1 (MQ=255)

                                                                                                                           tCGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTATGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGt       >  1:249834/1‑140 (MQ=255)

                                                                                                                            cGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGtt      <  1:602138/140‑1 (MQ=255)

                                                                                                                            cGCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGtt      <  1:1098043/140‑1 (MQ=255)

                                                                                                                            cGCTTCTG‑C‑GGGGGGGAAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGtt      <  2:379263/140‑1 (MQ=255)

                                                                                                                             gCTTCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGTTg     >  2:776847/1‑140 (MQ=255)

                                                                                                                               ttCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGTTGAg   >  2:758655/1‑140 (MQ=255)

                                                                                                                                tCTG‑CGGGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGTTGAGt  <  1:288058/140‑1 (MQ=255)

CCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTG‑C‑GGGGGGG‑AATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGTTGAGT  >  NC_000913/3001165‑3001431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A