Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,240,984	(G)_7→8	coding (1041/1245 nt)	sstT →	serine/threonine:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,240,977	1	.	G	96.2%	83.8 / ‑2.9	26	coding (1034/1245 nt)	sstT	serine/threonine:Na(+) symporter
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (3/22); total (3/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

ATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑C‑GGGGGGGTCTCT‑GCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTC  >  NC_000913/3240839‑3241109

atatGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑C‑g                                                                                                                                      <  2:586702/140‑1 (MQ=255)

      cgggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑C‑ggggggg                                                                                                                                <  1:743042/140‑1 (MQ=255)

        ggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑C‑ggggggggt                                                                                                                              <  2:533763/140‑3 (MQ=255)

             agcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTct‑g                                                                                                                         <  1:103590/140‑1 (MQ=255)

             agcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTct‑g                                                                                                                         <  1:201880/140‑1 (MQ=255)

             agcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTct‑g                                                                                                                         <  1:219132/140‑1 (MQ=255)

                         tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGGCTct‑tctgctggttccc                                                                                                             >  2:377076/1‑134 (MQ=255)

                           ccGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACt                                                                                                           <  1:377076/140‑1 (MQ=255)

                           ccGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGGCTCT‑GCTGCTGATcccccc                                                                                                           >  2:229594/1‑137 (MQ=255)

                              tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTct‑gctgctg                                                                                                                   <  2:422370/129‑1 (MQ=255)

                              tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTct‑gctgctg                                                                                                                   >  1:422370/1‑129 (MQ=255)

                              tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCa                                                                                                             >  1:174568/1‑135 (MQ=255)

                              tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCa                                                                                                             <  2:174568/135‑1 (MQ=255)

                              tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACTGGc                                                                                                        <  2:131104/140‑1 (MQ=255)

                              tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGGCTCT‑GCTGCTGATCCCCCTGGc                                                                                                        >  2:690326/1‑140 (MQ=255)

                              tgtTGACGCTGGCTGCGGTTAATACGCTGCGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGGGAAACCGGCCTGGAG‑GGGGGGGTCTCT‑GCTGCTGATCCCACTGGc                                                                                                        <  2:603517/140‑1 (MQ=255)

                                ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACTGGc                                                                                                        >  1:839207/1‑138 (MQ=255)

                                ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACTGGc                                                                                                        <  2:839207/138‑1 (MQ=255)

                                 tGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑C‑GGGGGGGTCTCT‑GCTGCTGATCCCACTGGCCTGt                                                                                                    <  1:170702/140‑1 (MQ=255)

                                   aCGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACTGGCCTGTa                                                                                                   >  1:515699/1‑140 (MQ=255)

                                          ctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACTGGCCTGTAATATGtt                                                                                            <  1:771033/140‑1 (MQ=255)

                                          ctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGGCTCT‑GCTGCTGATCCCACTCGCCTGTAATATGtt                                                                                            >  2:816189/1‑140 (MQ=255)

                                           tgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACTGGCCTGTAATATGTTc                                                                                           <  2:515699/140‑1 (MQ=255)

                                           tgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACTGGCCTATAATATGTTc                                                                                           <  1:631464/140‑1 (MQ=255)

                                                   aTACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGGCTCT‑GCTGCTGATCCCCCCGGCCTGTAATATGTTCGGGAtttt                                                                                   >  1:268443/1‑139 (MQ=255)

                                                      cGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGaa                                                                                <  1:513583/140‑1 (MQ=255)

                                                            gTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGatat                                                                          <  1:386591/140‑1 (MQ=255)

                                                            gTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGGCTCT‑GCCGCTGCTTCCCCTTGCCTGTTATATGTTTGGTAATTTGCACCatat                                                                          >  2:410155/1‑140 (MQ=255)

                                                               ttCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGc                                                                       <  1:276048/140‑1 (MQ=255)

                                                                     tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGGGTCT‑GCTGCTGATCCCTCTGGCCTGTAATATGTTTGGGATTTTGCACGATATCGCCATGcg                                                                 >  2:449102/1‑139 (MQ=255)

                                                                     tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGGCTCT‑GCTGCTGGTCCCCCTGGCCTGTAATATGTTCGGGATTTCGAACGATATCGCCCTGCa                                                                 >  2:133954/1‑140 (MQ=255)

                                                                     tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGGCTCT‑GCTGCTGATCCCCCTTGCCTGTAATATGTTCGGTATTTTGAACGATATCGCCATGCa                                                                 >  2:800387/1‑140 (MQ=255)

                                                                     tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑C‑GGGGGGGGGCCTCTCTTGTGGTCCCCCGGGCCTGTAATATATTCGGGTTTTCGGACGCCATCGCCCTGcc                                                                 >  2:64720/1‑139 (MQ=255)

                                                                                aCGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCg                                                      <  2:322388/140‑1 (MQ=255)

                                                                                 cGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGt                                                     <  1:64720/140‑1 (MQ=255)

                                                                                 cGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGt                                                     <  1:410155/140‑1 (MQ=255)

                                                                                              gAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGGCTCT‑GCTGCTGATCCCACTGGCCTGTAATATGTTTGGGATTTTGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcatc                                        >  2:192750/1‑140 (MQ=255)

                                                                                                      tggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCtt                                               <  1:323891/125‑1 (MQ=255)

                                                                                                      tggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCtt                                               >  2:323891/1‑125 (MQ=255)

                                                                                                       ggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTg                               <  1:800387/140‑1 (MQ=255)

                                                                                                             tctGTGTGCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGAc                         <  1:19046/140‑1 (MQ=255)

                                                                                                                   tgCCTGTGGCGCATCCGGCGTGG‑CGGGGGGGGGCTCT‑GCTGCTGATCCCACTGGCCTGTAATATGTTCGGGATTTTGAACGATATCGCCCTGCAGGGGGGTGCCGTCGGCTTTTTCATCGGCGGATTTCAGGGCTCTTGc                   >  2:19046/1‑140 (MQ=255)

                                                                                                                      cTGTGGCGCATCCGGCGTGG‑CGGGGGGGGGCTCT‑GCTGCTGATCCCCCTGGCCTGTAATATGTTTGGGATTTCGGACGATATCGCCCTGCAGGTGGTTGCCGTCGGCTTTTTTATCGGCGGATTGCAGGGCTCTTGCgga                >  2:489896/1‑138 (MQ=255)

                                                                                                                              cATCCGGCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCt        >  1:549888/1‑140 (MQ=255)

                                                                                                                                 agggCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGaa     <  1:528150/138‑1 (MQ=255)

                                                                                                                                    gCGTGG‑CGGGGGGGGTCTCT‑GCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAActc  <  1:133954/140‑1 (MQ=255)

ATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGG‑C‑GGGGGGGTCTCT‑GCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTC  >  NC_000913/3240839‑3241109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A