Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F73 I1 R1	26	81.7	2791006	99.4%	2774259	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,145,897	G→T	P122Q (CCA→CAA)	hybO ←	hydrogenase 2 small subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,145,897	0	G	T	98.8%	286.2 / ‑2.9	83	P122Q (CCA→CAA)	hybO	hydrogenase 2 small subunit
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (41/41); total (41/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

TCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACG  >  NC_000913/3145761‑3146031

tCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGa                                                                                                                                     <  1:416369/140‑1 (MQ=255)

tCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGa                                                                                                                                     >  1:911132/1‑140 (MQ=255)

tCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGa                                                                                                                                     <  2:642806/140‑1 (MQ=255)

tCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGa                                                                                                                                     <  1:212857/140‑1 (MQ=255)

tCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGa                                                                                                                                     >  2:1020236/1‑140 (MQ=255)

   aGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATgg                                                                                                                                  >  1:643479/1‑140 (MQ=255)

     cTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGaa                                                                                                                                <  2:990150/140‑1 (MQ=255)

        cGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCa                                                                                                                             >  1:980798/1‑140 (MQ=255)

         ggCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCAt                                                                                                                            <  2:1214652/140‑1 (MQ=255)

         ggCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCAt                                                                                                                            >  2:111330/1‑140 (MQ=255)

          gCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATc                                                                                                                           <  2:1301148/140‑1 (MQ=255)

            aaCACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATcca                                                                                                                         <  2:208345/140‑1 (MQ=255)

            aaCACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATcca                                                                                                                         <  2:348217/140‑1 (MQ=255)

             acacCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATccac                                                                                                                        <  2:156245/140‑1 (MQ=255)

             acacCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATccac                                                                                                                        <  1:887835/140‑1 (MQ=255)

              cacCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATccacc                                                                                                                       >  2:539630/1‑140 (MQ=255)

               cccGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATccacca                                                                                                                      <  2:287202/139‑1 (MQ=255)

               acCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATccacca                                                                                                                      <  2:81393/140‑1 (MQ=255)

               acCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATccacca                                                                                                                      <  1:500259/140‑1 (MQ=255)

               acCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATccacca                                                                                                                      <  1:81255/140‑1 (MQ=255)

                  gCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTa                                                                                                                   >  1:53828/1‑140 (MQ=255)

                   ccccACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATcc                                                                                                                          >  1:1357585/1‑132 (MQ=255)

                   ccccACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATcc                                                                                                                          <  2:1357585/132‑1 (MQ=255)

                    cccACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAac                                                                                                                 <  2:245913/140‑1 (MQ=255)

                        cGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACAt                                                                                                             >  1:776849/1‑140 (MQ=255)

                              gCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGcc                                                                                                       <  1:111330/140‑1 (MQ=255)

                                  gAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCttt                                                                                                   >  1:1306195/1‑140 (MQ=255)

                                   aaCCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTg                                                                                                  <  1:1028795/140‑1 (MQ=255)

                                   aaCCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTg                                                                                                  <  1:431856/140‑1 (MQ=255)

                                         tAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTtc                                                                                            >  1:768171/1‑140 (MQ=255)

                                         tAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTtc                                                                                            >  2:689463/1‑140 (MQ=255)

                                             gATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCga                                                                                        <  2:326194/140‑1 (MQ=255)

                                             gATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCga                                                                                        <  1:1146063/140‑1 (MQ=255)

                                              aTAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCgag                                                                                       <  1:539630/140‑1 (MQ=255)

                                                  tGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCg                                                                                   <  1:970402/140‑1 (MQ=255)

                                                  tGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTACTCGAGAGCg                                                                                   >  2:408547/1‑140 (MQ=255)

                                                   ggCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGt                                                                                  >  1:711112/1‑140 (MQ=255)

                                                    gctgctGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGtt                                                                                 <  2:159150/140‑1 (MQ=255)

                                                      tgctgcGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTAt                                                                               >  2:1256389/1‑140 (MQ=255)

                                                      tgctgcGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTAt                                                                               >  2:1276622/1‑140 (MQ=255)

                                                        ctgcGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTAtgt                                                                             >  1:242866/1‑140 (MQ=255)

                                                         tgcGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTAtgtt                                                                            >  1:848466/1‑140 (MQ=255)

                                                           cgcCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTAtgtttg                                                                          <  1:906117/140‑1 (MQ=255)

                                                           cgcCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTAtgtttg                                                                          >  2:1197970/1‑140 (MQ=255)

                                                           cgcCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTAtgtttg                                                                          <  1:684770/140‑1 (MQ=255)

                                                            gcCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTAtgtttgt                                                                         >  1:1317753/1‑140 (MQ=255)

                                                            gcCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTAtgtttgt                                                                         <  2:848466/140‑1 (MQ=255)

                                                                 ccgccgCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTctct                                                                    <  2:776849/140‑1 (MQ=255)

                                                                               aTGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGAcc                                                      <  2:1379626/140‑1 (MQ=255)

                                                                                    aTCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAgg                                                 >  2:993867/1‑140 (MQ=255)

                                                                                      ccACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAggcg                                               <  1:609428/140‑1 (MQ=255)

                                                                                        acaATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAggcggc                                             >  1:163090/1‑140 (MQ=255)

                                                                                           aTCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGa                                          <  2:1317753/140‑1 (MQ=255)

                                                                                           aTCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGa                                          >  1:1035778/1‑140 (MQ=255)

                                                                                           aTCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGa                                          <  2:1306195/140‑1 (MQ=255)

                                                                                            tCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGaa                                         >  1:1372983/1‑140 (MQ=255)

                                                                                            tCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGaa                                         >  1:907165/1‑140 (MQ=255)

                                                                                             cGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCATTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGaag                                        <  1:237707/140‑2 (MQ=255)

                                                                                              ggCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAg                                       >  2:521728/1‑140 (MQ=255)

                                                                                                cTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCa                                     <  2:630922/140‑1 (MQ=255)

                                                                                                cTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCa                                     >  2:414514/1‑140 (MQ=255)

                                                                                                   aCCGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACtt                                  <  2:402901/140‑1 (MQ=255)

                                                                                                     cGGCAACCATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCg                                <  2:163090/140‑1 (MQ=255)

                                                                                                           ccATGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATAc                          <  1:1020236/140‑1 (MQ=255)

                                                                                                             aTGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTc                        <  2:1035778/140‑1 (MQ=255)

                                                                                                              tGCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTcc                       >  1:769663/1‑140 (MQ=255)

                                                                                                               gCAATAAATACCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCa                      >  2:571758/1‑140 (MQ=255)

                                                                                                                  ataaataCCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCagag                   <  1:711744/140‑1 (MQ=255)

                                                                                                                     aataCCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAgagaga                <  1:689463/140‑1 (MQ=255)

                                                                                                                        aCCGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAg             >  1:1294540/1‑140 (MQ=255)

                                                                                                                          cGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATa              >  1:787558/1‑137 (MQ=255)

                                                                                                                          cGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATa              <  2:787558/137‑1 (MQ=255)

                                                                                                                          cGTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGtc           >  1:1151582/1‑140 (MQ=255)

                                                                                                                           gTTATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGtct          >  2:570040/1‑140 (MQ=255)

                                                                                                                            ttATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGtctc         >  1:261053/1‑140 (MQ=255)

                                                                                                                            ttATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGtctc         >  2:39525/1‑140 (MQ=255)

                                                                                                                            ttATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGtctc         >  1:660223/1‑140 (MQ=255)

                                                                                                                             tATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTcc        >  1:747053/1‑140 (MQ=255)

                                                                                                                             tATCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTcc        >  1:991663/1‑140 (MQ=255)

                                                                                                                               tCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAg      >  2:298917/1‑140 (MQ=255)

                                                                                                                               tCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAg      >  2:874348/1‑140 (MQ=255)

                                                                                                                               tCTTTTAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAg      >  2:366545/1‑140 (MQ=255)

                                                                                                                                   ttAATTGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCAAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACg  <  2:242866/140‑1 (MQ=255)

TCCAGCTGCGGCAACACCGCCCCACGCAGAGCAGGAACCGATAGCGATAATGGCTGCTGCGCCTTCCGCCGCTTTGCGGATGTGATCCACAATCGGCTCACCGGCAACCATGCAATAAATACCGTTATCTTTTAATGGGATGGAACCATCCACCACTAACACATACTGCCCTTTGTACTTCTCGAGAGCGTTATGTTTGTTCTCTTCGACCTGATGACCGAAGGCGGCGGAAAGCACTTCGTGATACTCCAGAGAGATAGTCTCCAGTACG  >  NC_000913/3145761‑3146031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A