Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F73 I1 R1	26	81.7	2791006	99.4%	2774259	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,370,163	G→T	S63* (TCG→TAG)	nanK ←	N‑acetylmannosamine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,370,163	0	G	T	98.2%	192.5 / ‑3.7	55	S63* (TCG→TAG)	nanK	N‑acetylmannosamine kinase
Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (20/34); total (21/34)
Fisher's exact test for biased strand distribution p-value = 3.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCGAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAAT  >  NC_000913/3370030‑3370299

cTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGa                                                                                                                                    <  1:1037181/140‑1 (MQ=255)

   cgcgTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATgg                                                                                                                                 >  2:1188967/1‑140 (MQ=255)

        cgtTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAAcc                                                                                                                            <  2:836240/140‑1 (MQ=255)

          ttAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGGCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCg                                                                                                                          <  2:574073/140‑1 (MQ=255)

          ttAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCg                                                                                                                          <  2:940712/140‑1 (MQ=255)

           tAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGc                                                                                                                         <  2:20083/140‑1 (MQ=255)

            aaTGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCt                                                                                                                        <  1:1095282/140‑1 (MQ=255)

                 cAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGATTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCa                                                                                                                   <  1:999651/140‑1 (MQ=255)

                  aaTGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCAt                                                                                                                  <  2:651713/140‑1 (MQ=255)

                  aaTGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACAGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCAt                                                                                                                  <  1:665900/140‑1 (MQ=255)

                    tGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCa                                                                                                                   <  2:603982/137‑1 (MQ=255)

                    tGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCa                                                                                                                   >  1:603982/1‑137 (MQ=255)

                    tGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGa                                                                                                                >  2:666234/1‑140 (MQ=255)

                         ggCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTg                                                                                                           >  2:1330083/1‑140 (MQ=255)

                          gCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGc                                                                                                          >  1:5755/1‑140 (MQ=255)

                           cAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCa                                                                                                         <  1:372329/140‑1 (MQ=255)

                           cAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCa                                                                                                         <  1:737831/140‑1 (MQ=255)

                           cAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCa                                                                                                         <  1:1188967/140‑1 (MQ=255)

                                  gTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGgaga                                                                                                  <  1:823951/140‑1 (MQ=255)

                                         gttCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAAt                                                                                           <  2:369953/140‑1 (MQ=255)

                                          ctCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCACGGTTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATg                                                                                          >  2:760816/2‑140 (MQ=255)

                                           tCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGc                                                                                         <  2:1321000/140‑1 (MQ=255)

                                            ccAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCg                                                                                        >  1:979701/1‑140 (MQ=255)

                                                cGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATa                                                                                    <  1:1279365/140‑1 (MQ=255)

                                                  ttttGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAg                                                                                  >  2:138471/1‑140 (MQ=255)

                                                           aCGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGc                                                                         <  2:1222373/140‑1 (MQ=255)

                                                            cGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCa                                                                        >  1:460778/1‑140 (MQ=255)

                                                             ggAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCaa                                                                       <  1:1249908/140‑1 (MQ=255)

                                                             ggAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCaa                                                                       <  1:666234/140‑1 (MQ=255)

                                                               aaaGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAgg                                                                     <  1:679831/140‑1 (MQ=255)

                                                                     tAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTg                                                               >  1:1024142/1‑140 (MQ=255)

                                                                     tAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTg                                                               >  2:570003/1‑140 (MQ=255)

                                                                       gCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGgt                                                             >  1:154904/1‑140 (MQ=255)

                                                                           tCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCGAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTct                                                         >  1:903662/1‑140 (MQ=255)

                                                                             caccaAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTctgg                                                       >  1:561616/1‑140 (MQ=255)

                                                                             caccaAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTctgg                                                       >  1:1252265/1‑140 (MQ=255)

                                                                                 aaGATTATGCGGATTAAGCGCCGGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTctggctgg                                                   <  2:160227/140‑1 (MQ=255)

                                                                                 aaGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTctggctgg                                                   <  1:123591/140‑1 (MQ=255)

                                                                                 aaGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTctggctgg                                                   <  2:794647/140‑1 (MQ=255)

                                                                                 aaGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTctggctgg                                                   <  2:158050/140‑1 (MQ=255)

                                                                                 aaGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTctggctgg                                                   <  1:947658/140‑1 (MQ=255)

                                                                                        tGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGt                                            >  1:429169/1‑140 (MQ=255)

                                                                                           ggATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAgg                                         <  2:151613/140‑1 (MQ=255)

                                                                                             aTTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGaa                                       >  2:1239331/1‑140 (MQ=255)

                                                                                              ttAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAg                                      >  2:900645/1‑140 (MQ=255)

                                                                                              ttAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAg                                      <  1:870346/140‑1 (MQ=255)

                                                                                                     ccAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCacga                               <  1:1176361/140‑1 (MQ=255)

                                                                                                      cAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCacgac                              <  1:138471/140‑1 (MQ=255)

                                                                                                      cAGCAAGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACACAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCacgac                              <  1:1330083/140‑1 (MQ=255)

                                                                                                           aGCTGCCGTCACGGATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATcg                         <  2:460778/140‑1 (MQ=255)

                                                                                                                        gATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGt            <  2:1024142/140‑1 (MQ=255)

                                                                                                                        gATTATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGt            <  1:714547/140‑1 (MQ=255)

                                                                                                                          ttATCCCGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCa          >  1:707669/1‑140 (MQ=255)

                                                                                                                              cccGGTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAgcgc      >  1:161102/1‑140 (MQ=255)

                                                                                                                                  gTCTAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAAt  >  2:466553/1‑140 (MQ=255)

CTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATTATGCGGATTAAGCGCCAGCAAGCTGCCGTCACGGATTATCCCGGTCGAAGCGATGGCAACCCGCTGCGCATGAGCTTGCAACGGAGAGACTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAAT  >  NC_000913/3370030‑3370299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A