Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F73 I1 R1	26	81.7	2791006	99.4%	2774259	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,966,245	G→T	Q5H (CAG→CAT)	rhoL →	rho operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,966,245	0	G	T	100.0%	301.7 / NA	85	Q5H (CAG→CAT)	rhoL	rho operon leader peptide
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (45/40); total (45/40)

GTGCCCCGTCGCTAAAAACTGGACGCCCGGCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAA  >  NC_000913/3966108‑3966378

gTGCCCCGTCGCTAAAAACTGGACGCCCGGCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACatat                                                                                                                                     >  2:1069422/1‑140 (MQ=255)

     ccGTCGCTAAAAACTGGACGCCCGGCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTg                                                                                                                                <  1:758510/140‑1 (MQ=255)

     ccGTCGCTAAAAACTGGACGCCCGGCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTg                                                                                                                                >  1:809308/1‑140 (MQ=255)

     ccGTCGCTAAAAACTGGACGCCCGGCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTg                                                                                                                                >  1:1061274/1‑140 (MQ=255)

        tCGCTAAAAACTGGACGCCCGGCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCt                                                                                                                             >  1:402029/1‑140 (MQ=255)

         cGCTAAAAACTGGACGCCCGGCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTc                                                                                                                            <  2:401937/140‑1 (MQ=255)

           cTAAAAACTGGACGCCCGGCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGt                                                                                                                          <  2:253265/140‑1 (MQ=255)

             aaaaaCTGGACGCCCGGCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCa                                                                                                                        <  2:1349710/140‑1 (MQ=255)

             aaaaaCTGGACGCCCGGCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCa                                                                                                                        <  2:1011931/140‑1 (MQ=255)

             aaaaaCTGGACGCCCGGCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCa                                                                                                                        <  1:359349/140‑1 (MQ=255)

              aaaaCTGGACGCCCGGCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCAc                                                                                                                       <  1:887208/140‑1 (MQ=255)

                     gACGCCCGGCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATcc                                                                                                                >  1:432826/1‑140 (MQ=255)

                      aCGCCCGGCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCg                                                                                                               >  1:1323560/1‑140 (MQ=255)

                          ccGGCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCtt                                                                                                           >  2:197191/1‑140 (MQ=255)

                          ccGGCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCtt                                                                                                           >  2:567186/1‑140 (MQ=255)

                          ccGGCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCtt                                                                                                           >  1:1051458/1‑140 (MQ=255)

                          ccGGCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCtt                                                                                                           >  1:531852/1‑140 (MQ=255)

                            ggCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGt                                                                                                         <  1:1009167/140‑1 (MQ=255)

                            ggCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGt                                                                                                         <  1:906373/140‑1 (MQ=255)

                                   gTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCa                                                                                                  <  2:743226/140‑1 (MQ=255)

                                      aTGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGtt                                                                                               <  1:740689/140‑1 (MQ=255)

                                        gCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCt                                                                                             <  2:421522/140‑1 (MQ=255)

                                          tAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGc                                                                                           <  1:793668/140‑1 (MQ=255)

                                          tAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGc                                                                                           <  1:534770/140‑1 (MQ=255)

                                           aaCTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCg                                                                                          <  2:402029/140‑1 (MQ=255)

                                            aCTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGt                                                                                         <  1:59552/140‑1 (MQ=255)

                                             cTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTa                                                                                        >  1:1055525/1‑140 (MQ=255)

                                             cTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTa                                                                                        <  1:866405/140‑1 (MQ=255)

                                             cTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTa                                                                                        >  2:1212512/1‑140 (MQ=255)

                                             cTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTa                                                                                        <  2:1061274/140‑1 (MQ=255)

                                              ttAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTAc                                                                                       <  2:835912/140‑1 (MQ=255)

                                               tAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTAct                                                                                      >  2:553153/1‑140 (MQ=255)

                                               tAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTAct                                                                                      <  1:692957/140‑1 (MQ=255)

                                               tAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTAct                                                                                      <  1:659682/140‑1 (MQ=255)

                                               tAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTAct                                                                                      <  2:531852/140‑1 (MQ=255)

                                               tAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTAct                                                                                      <  2:1147413/140‑1 (MQ=255)

                                                 gtgtTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTActct                                                                                    >  2:1108998/1‑140 (MQ=255)

                                                 gtgtTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTActct                                                                                    >  2:766354/1‑140 (MQ=255)

                                                  tgtTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCa                                                                                                  <  1:168200/125‑1 (MQ=255)

                                                  tgtTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCa                                                                                                  >  2:168200/1‑125 (MQ=255)

                                                  tgtTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTc                                                                                              >  2:1370320/1‑129 (MQ=255)

                                                  tgtTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTc                                                                                              <  1:1370320/129‑1 (MQ=255)

                                                  tgtTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACtctc                                                                                   >  1:907003/1‑140 (MQ=255)

                                                   gtTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTcc                                                                                  <  2:961800/140‑1 (MQ=255)

                                                    ttGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCt                                                                                 >  1:1363964/1‑140 (MQ=255)

                                                     tGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCtg                                                                                <  1:798024/140‑1 (MQ=255)

                                                        cTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGa                                                                             >  1:1291354/1‑140 (MQ=255)

                                                           cGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCa                                                                          >  2:1030839/1‑140 (MQ=255)

                                                                   aCATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGa                                                                  >  2:31143/1‑140 (MQ=255)

                                                                       aCCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAg                                                              >  2:680562/1‑140 (MQ=255)

                                                                       aCCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAg                                                              >  1:371994/1‑140 (MQ=255)

                                                                       aCCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAg                                                              >  2:1131269/1‑140 (MQ=255)

                                                                       aCCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAg                                                              >  1:758458/1‑140 (MQ=255)

                                                                       aCCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAg                                                              >  1:813254/1‑140 (MQ=255)

                                                                          ttattaAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACa                                                           >  2:723556/1‑140 (MQ=255)

                                                                           tattaAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACAt                                                          <  2:432826/140‑1 (MQ=255)

                                                                            attaAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGaa                                                                 <  2:874340/132‑1 (MQ=255)

                                                                            attaAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGaa                                                                 >  1:874340/1‑132 (MQ=255)

                                                                            attaAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATg                                                         >  2:596086/1‑140 (MQ=255)

                                                                            attaAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATg                                                         >  1:85989/1‑140 (MQ=255)

                                                                              taAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAg                                                       >  2:679413/1‑140 (MQ=255)

                                                                                  tttGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGa                                                   <  2:907003/140‑1 (MQ=255)

                                                                                  tttGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGa                                                   <  2:102290/140‑1 (MQ=255)

                                                                                   ttGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCTTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGAt                                                  <  1:1082065/140‑1 (MQ=255)

                                                                                   ttGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGAt                                                  >  1:277093/1‑140 (MQ=255)

                                                                                   ttGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGAt                                                  >  2:1016219/1‑140 (MQ=255)

                                                                                   ttGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGAt                                                  >  2:1143044/1‑140 (MQ=255)

                                                                                   ttGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGAt                                                  >  1:1354417/1‑140 (MQ=255)

                                                                                      aaTCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGAc                                               >  1:1077361/1‑140 (MQ=255)

                                                                                       aTCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGAcc                                              >  2:377276/1‑140 (MQ=255)

                                                                                                tttCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAgg                                     >  1:159999/1‑140 (MQ=255)

                                                                                                 ttCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGc                                    <  2:920716/140‑1 (MQ=255)

                                                                                                 ttCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGc                                    <  1:680562/140‑1 (MQ=255)

                                                                                                     aaCGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATgg                                <  1:553153/140‑1 (MQ=255)

                                                                                                      aCGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGga                               <  1:197191/140‑1 (MQ=255)

                                                                                                       cGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGgat                              >  1:601853/1‑140 (MQ=255)

                                                                                                       cGCTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGgat                              <  2:1051458/140‑1 (MQ=255)

                                                                                                       cGCTTCCCGTTTTATCTTAAATGCGAAGGGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGgat                              >  2:669044/1‑140 (MQ=255)

                                                                                                         cTTCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGgatga                            <  2:1291354/140‑1 (MQ=255)

                                                                                                           tCCCGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATc                          <  1:31143/140‑1 (MQ=255)

                                                                                                            cccGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATcc                         >  2:66567/1‑140 (MQ=255)

                                                                                                             ccGTTTTATCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCt                        >  2:362375/1‑140 (MQ=255)

                                                                                                                    aTCTTAAATGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATAc                 >  1:685893/1‑140 (MQ=255)

                                                                                                                          aaTGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTca           >  2:167790/1‑140 (MQ=255)

                                                                                                                          aaTGCGAAGTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTca           <  1:362375/140‑1 (MQ=255)

                                                                                                                                  gTGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTa   <  1:113330/140‑1 (MQ=255)

                                                                                                                                   tGAACATATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTaa  >  1:754254/1‑140 (MQ=255)

GTGCCCCGTCGCTAAAAACTGGACGCCCGGCGTGAGTCATGCTAACTTAGTGTTGACTTCGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAA  >  NC_000913/3966108‑3966378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A