Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F73 I1 R1	26	81.7	2791006	99.4%	2774259	139.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,085,782	C→A	G14V (GGC→GTC)	fdoG ←	formate dehydrogenase O subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,085,782	0	C	A	100.0%	184.1 / NA	53	G14V (GGC→GTC)	fdoG	formate dehydrogenase O subunit alpha
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (31/22); total (31/22)

GCCCACAGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGCCAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCG  >  NC_000913/4085648‑4085917

gCCCACAGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCTGTGGTGCCTGCCATACCGACAgcg                                                                                                                                    >  2:900856/1‑140 (MQ=255)

gCCCACAGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAgcg                                                                                                                                    >  2:1101182/1‑140 (MQ=255)

gCCCACAGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTACAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAgcg                                                                                                                                    >  2:70999/1‑140 (MQ=255)

 cccACAGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAgcgc                                                                                                                                   <  2:1077636/140‑1 (MQ=255)

  ccACAGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCa                                                                                                                                  >  1:1284158/1‑140 (MQ=255)

   cacaGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAg                                                                                                                                 >  1:1037676/1‑140 (MQ=255)

   cacaGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAg                                                                                                                                 >  2:744794/1‑140 (MQ=255)

      aGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATc                                                                                                                              >  2:1285737/1‑140 (MQ=255)

                   aTAGGTGCAGGTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGcc                                                                                                                 <  1:1056075/140‑1 (MQ=255)

                         gCAGGTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTctgc                                                                                                           >  2:1283392/1‑140 (MQ=255)

                         gCAGGTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTctgc                                                                                                           >  2:395021/1‑140 (MQ=255)

                          cAGGTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTctgct                                                                                                          <  1:1020526/140‑1 (MQ=255)

                            ggTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGa                                                                                                        >  2:279056/1‑140 (MQ=255)

                            ggTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGa                                                                                                        <  2:774818/140‑1 (MQ=255)

                             gTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTAGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGAc                                                                                                       <  1:698632/140‑1 (MQ=255)

                                 tACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGc                                                                                                   >  2:456789/1‑140 (MQ=255)

                                  aCGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGa                                                                                                        <  1:247356/134‑1 (MQ=255)

                                  aCGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGa                                                                                                        >  2:247356/1‑134 (MQ=255)

                                      gTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGa                                                                                              <  1:1232579/140‑1 (MQ=255)

                                       tttCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGAt                                                                                             <  1:259793/140‑1 (MQ=255)

                                       tttCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGAt                                                                                             >  1:542613/1‑140 (MQ=255)

                                        ttCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGAtt                                                                                            >  2:61830/1‑140 (MQ=255)

                                         tCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTg                                                                                           >  2:1376539/1‑140 (MQ=255)

                                                         gTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACAt                                                                           <  1:456789/140‑1 (MQ=255)

                                                            tataCTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGt                                                                        <  1:1035289/140‑1 (MQ=255)

                                                            tataCTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGt                                                                        >  2:471850/1‑140 (MQ=255)

                                                             ataCTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTc                                                                       <  2:1284158/140‑1 (MQ=255)

                                                               aCTGCCGGGTTTCCGCGAGGGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCAc                                                                     >  1:858402/1‑140 (MQ=255)

                                                                  gCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTc                                                                  <  1:112713/140‑1 (MQ=255)

                                                                  gCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTc                                                                  <  1:1360154/140‑1 (MQ=255)

                                                                  gCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTc                                                                  <  1:63626/140‑1 (MQ=255)

                                                                    cGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTccc                                                                >  2:1363714/1‑140 (MQ=255)

                                                                    cGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTccc                                                                >  2:1260202/1‑140 (MQ=255)

                                                                    cGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTccc                                                                >  2:731425/1‑140 (MQ=255)

                                                                    cGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTACTAGTTTCGACATTGTCACGTcac                                                                >  1:884252/1‑138 (MQ=255)

                                                                      ggcttCCGCGAGTGCTACGATGGGCGCAAAACCCAGTGCCGCTTCCATGGTGCCTGTCATACCGACAGCGCAGAACTCAAAGCACGGCCTTCCGCTGACCTGCATGGATTGCTACTTGTTTCGACATTGTCACGTCCAAt                                                              >  2:252097/4‑140 (MQ=255)

                                                                                  tGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCtt                                                  <  1:731425/140‑1 (MQ=255)

                                                                                      aCGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTtgctg                                              <  1:1376539/140‑1 (MQ=255)

                                                                                        gCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCg                                            <  1:2129/140‑1 (MQ=255)

                                                                                            ggTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCg                                            <  1:308550/136‑1 (MQ=255)

                                                                                            ggTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCg                                            >  2:308550/1‑136 (MQ=255)

                                                                                            ggTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGtgtg                                        >  1:473470/1‑140 (MQ=255)

                                                                                            ggTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGtgtg                                        >  2:381465/1‑140 (MQ=255)

                                                                                            ggTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCCTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGtgtg                                        >  2:1067102/1‑140 (MQ=255)

                                                                                              tGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCa                                      <  2:580156/140‑1 (MQ=255)

                                                                                                  aaccccAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAggga                                  <  1:1116843/137‑1 (MQ=255)

                                                                                                    aCCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAgggagt                                <  1:1101182/140‑1 (MQ=255)

                                                                                                               cTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTAttt                     >  2:1153562/1‑140 (MQ=255)

                                                                                                               cTGCCGTGGTGCCTGCCATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTAttt                     >  2:717663/1‑140 (MQ=255)

                                                                                                                          cctgccATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGa          >  2:1178884/1‑140 (MQ=255)

                                                                                                                          cctgccATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGa          <  2:840660/140‑1 (MQ=255)

                                                                                                                            tgccATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAg        >  1:618140/1‑140 (MQ=255)

                                                                                                                            tgccATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAg        >  1:387231/1‑140 (MQ=255)

                                                                                                                            tgccATACCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAg        >  1:179474/1‑140 (MQ=255)

                                                                                                                                  aCCGACAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCg  >  2:1258556/1‑140 (MQ=255)

GCCCACAGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGCTGCCGTGGTGCCTGCCATACCGCCAGCGCAGATCTTAAAGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCG  >  NC_000913/4085648‑4085917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A