Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F73 I1 R1	28	94.5	3320338	99.3%	3297095	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,223,176	G→T	D182Y (GAC→TAC)	ebgA →	evolved beta‑D‑galactosidase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,223,176	0	G	T	98.9%	301.1 / ‑3.0	87	D182Y (GAC→TAC)	ebgA	evolved beta‑D‑galactosidase subunit alpha
Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (51/35); total (52/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.52e-01

TATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGC  >  NC_000913/3223044‑3223312

tATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTctac                                                                                                                                   <  2:1024353/140‑1 (MQ=255)

        aGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTgg                                                                                                                           <  1:652028/140‑1 (MQ=255)

         gTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGTGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGa                                                                                                                          >  1:15226/1‑140 (MQ=255)

         gTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGa                                                                                                                          >  1:915354/1‑140 (MQ=255)

         gTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGa                                                                                                                          <  2:25453/140‑1 (MQ=255)

           cTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAg                                                                                                                        <  1:508460/140‑1 (MQ=255)

            tATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGa                                                                                                                       >  1:733331/1‑140 (MQ=255)

            tATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGa                                                                                                                       >  1:1252141/1‑140 (MQ=255)

            tATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGa                                                                                                                       >  1:186552/1‑140 (MQ=255)

             aTGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGAc                                                                                                                      <  2:154721/140‑1 (MQ=255)

              tGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGTGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGAcc                                                                                                                     <  2:15226/140‑1 (MQ=255)

              tGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGAcc                                                                                                                     <  2:255175/140‑1 (MQ=255)

              tGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGAcc                                                                                                                     <  1:853636/140‑1 (MQ=255)

              tGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGAcc                                                                                                                     <  1:583632/140‑1 (MQ=255)

              tGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGAcc                                                                                                                     <  1:785953/140‑1 (MQ=255)

              tGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGAcc                                                                                                                     <  1:1138045/140‑1 (MQ=255)

                 tAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAgg                                                                                                                  <  2:186552/140‑1 (MQ=255)

                   aCGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGat                                                                                                                <  1:934818/140‑1 (MQ=255)

                    cGGTCAGTATGTGGGTTTCAGCAAGGGCGGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGata                                                                                                               >  2:350504/1‑140 (MQ=255)

                    cGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGAcc                                                                                                                     <  1:612172/134‑1 (MQ=255)

                    cGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGAcc                                                                                                                     >  2:612172/1‑134 (MQ=255)

                    cGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGata                                                                                                               >  1:198565/1‑140 (MQ=255)

                      gTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGAc                                                                                                                      <  1:529690/131‑1 (MQ=255)

                      gTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGAc                                                                                                                      >  2:529690/1‑131 (MQ=255)

                      gTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATAtg                                                                                                             >  1:1322370/1‑140 (MQ=255)

                      gTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATAtg                                                                                                             >  1:813827/1‑140 (MQ=255)

                      gTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATAtg                                                                                                             >  2:470025/1‑140 (MQ=255)

                       tCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATAtgt                                                                                                            >  1:869725/1‑140 (MQ=255)

                        cAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATAtgtg                                                                                                           >  2:457330/1‑140 (MQ=255)

                        cAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATAtgtg                                                                                                           >  2:894150/1‑140 (MQ=255)

                             tgtgGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTggtggt                                                                                                      <  2:1386331/140‑1 (MQ=255)

                               tgGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATAtgtg                                                                                                           <  1:602860/133‑1 (MQ=255)

                               tgGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATAtgtg                                                                                                           >  2:602860/1‑133 (MQ=255)

                                gggTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAg                                                                                                   <  1:894150/140‑1 (MQ=255)

                                 ggTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTggtggt                                                                                                      <  1:1582818/136‑1 (MQ=255)

                                 ggTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTggtggt                                                                                                      >  2:1582818/1‑136 (MQ=255)

                                     tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCgggg                                                                                              >  2:1524372/1‑140 (MQ=255)

                                     tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCgggg                                                                                              >  2:519498/1‑140 (MQ=255)

                                     tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCgggg                                                                                              >  2:756722/1‑140 (MQ=255)

                                     tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCgggg                                                                                              >  1:1297030/1‑140 (MQ=255)

                                     tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCgggg                                                                                              >  1:1195905/1‑140 (MQ=255)

                                     tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCgggg                                                                                              >  2:1569720/1‑140 (MQ=255)

                                     tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGGGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGT‑GTGGTCAGCGGGGa                                                                                             >  1:1472325/1‑140 (MQ=255)

                                             ggCGGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                                                      <  1:350504/140‑1 (MQ=255)

                                             ggCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                                                      <  1:756722/140‑1 (MQ=255)

                                             ggCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                                                      <  1:251783/140‑1 (MQ=255)

                                             ggCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                                                      <  1:116901/140‑1 (MQ=255)

                                               cAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcgcg                                                                                    <  1:634537/140‑1 (MQ=255)

                                                       tGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTAt                                                                            >  1:964645/1‑140 (MQ=255)

                                                            gCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGt                                                                       <  2:1195905/140‑1 (MQ=255)

                                                            gCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGt                                                                       <  2:1317067/140‑1 (MQ=255)

                                                             cAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTc                                                                      <  1:1524372/140‑1 (MQ=255)

                                                                    tGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAac                                                               >  2:416221/1‑140 (MQ=255)

                                                                        aTCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCt                                                           >  2:1460006/1‑140 (MQ=255)

                                                                        aTCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCt                                                           <  1:527731/140‑1 (MQ=255)

                                                                          cAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTaa                                                         >  2:1516981/1‑140 (MQ=255)

                                                                           aGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAAc                                                        >  2:1569788/1‑140 (MQ=255)

                                                                            gcgcGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAAAACCTAACg                                                       >  2:17202/1‑140 (MQ=255)

                                                                                  tGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATAtt                                                 <  2:198565/140‑1 (MQ=255)

                                                                                  tGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATAtt                                                 >  2:700735/1‑140 (MQ=255)

                                                                                  tGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATAtt                                                 <  1:700735/140‑1 (MQ=255)

                                                                                              gCGACAACCTGTTGTGTGTGTGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACt                                     >  2:1383845/1‑140 (MQ=255)

                                                                                              gCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTc                                        >  1:714071/1‑137 (MQ=255)

                                                                                              gCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTc                                        <  2:714071/137‑1 (MQ=255)

                                                                                              gCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACt                                     >  2:1336425/1‑140 (MQ=255)

                                                                                              gCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACt                                     >  1:1114211/1‑140 (MQ=255)

                                                                                              gCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACt                                     >  1:923222/1‑140 (MQ=255)

                                                                                              gCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACt                                     >  1:496228/1‑140 (MQ=255)

                                                                                                   aaCCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCg                                >  1:396075/1‑140 (MQ=255)

                                                                                                             gtgtgCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACttt                      >  1:118890/1‑140 (MQ=255)

                                                                                                              tgtgCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTg                     >  2:615844/1‑140 (MQ=255)

                                                                                                               gtgCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGa                    >  1:759841/1‑140 (MQ=255)

                                                                                                                tgCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGAc                   >  2:60216/1‑140 (MQ=255)

                                                                                                                tgCGCGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGAc                   >  1:1063612/1‑140 (MQ=255)

                                                                                                                 gcgcGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACg                  <  2:496228/140‑1 (MQ=255)

                                                                                                                 gcgcGTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACg                  <  1:494802/140‑1 (MQ=255)

                                                                                                                   gcgTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGaa                >  1:1261687/1‑140 (MQ=255)

                                                                                                                   gcgTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGaa                >  1:224855/1‑140 (MQ=255)

                                                                                                                   gcgTGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGaa                <  2:1322370/140‑1 (MQ=255)

                                                                                                                      tGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGcc             >  2:1497097/1‑140 (MQ=255)

                                                                                                                      tGATGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGcc             >  2:1308893/1‑140 (MQ=255)

                                                                                                                        aTGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTa           >  1:1059507/1‑140 (MQ=255)

                                                                                                                        aTGCAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTa           >  2:877490/1‑140 (MQ=255)

                                                                                                                           cAGTGGGCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTg        <  2:1436353/140‑1 (MQ=255)

                                                                                                                               gggCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGAt    >  1:653/1‑140 (MQ=255)

                                                                                                                                ggCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATg   <  1:1516981/140‑1 (MQ=255)

                                                                                                                                ggCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATg   >  1:727418/1‑140 (MQ=255)

                                                                                                                                 gCGTACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGc  >  1:350548/1‑140 (MQ=255)

TATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGC  >  NC_000913/3223044‑3223312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A