Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F73 I1 R1	28	94.5	3320338	99.3%	3297095	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,537,408	C→A	R8R (CGC→CGA)	yhgF →	putative RNA‑binding protein YhgF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,537,408	0	C	A	100.0%	176.9 / NA	52	R8R (CGC→CGA)	yhgF	putative RNA‑binding protein YhgF
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (26/26); total (26/26)

GTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCGTTTATCGCACGTTATCGTAAGGAAATCACCGGCGGTCTGGATGACACGCAG  >  NC_000913/3537277‑3537540

gTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGc                                                                                                                              <  2:71771/140‑1 (MQ=255)

gTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGc                                                                                                                              <  2:1280253/140‑1 (MQ=255)

   aaaCCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCggg                                                                                                                           <  1:839458/140‑1 (MQ=255)

     aCCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTg                                                                                                                         >  2:1093120/1‑140 (MQ=255)

               agagTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGgc                                                                                                               <  2:976088/140‑1 (MQ=255)

               agagTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGgc                                                                                                               <  1:59224/140‑1 (MQ=255)

                   tCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGgcgcgc                                                                                                           >  2:1277067/1‑140 (MQ=255)

                       aaCCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCgg                                                                                                       >  2:1232847/1‑140 (MQ=255)

                        aCCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGa                                                                                                      >  1:889644/1‑140 (MQ=255)

                         cctcctGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGaa                                                                                                     <  1:397657/140‑1 (MQ=255)

                             ctGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGgc                                                                                                               >  1:197973/1‑126 (MQ=255)

                             ctGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGgc                                                                                                               <  2:197973/126‑1 (MQ=255)

                             ctGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAgg                                                                                                 >  2:416627/1‑140 (MQ=255)

                             ctGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAgg                                                                                                 >  1:1425801/1‑140 (MQ=255)

                              tGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGt                                                                                                <  2:171340/140‑1 (MQ=255)

                                   aTGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACg                                                                                           <  1:961571/140‑1 (MQ=255)

                                    tggctggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCTGAATTATTGCGGGTGAAATTCAGCCGCGCCCGGAACAGGTTGACGc                                                                                          >  2:215369/1‑140 (MQ=255)

                                    tggctggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGc                                                                                          >  2:6389/1‑140 (MQ=255)

                                    tggctggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAACATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGATCAAGTTGACGc                                                                                          <  2:374847/140‑1 (MQ=255)

                                     ggctggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCt                                                                                         >  1:254589/1‑140 (MQ=255)

                                       ctggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGc                                                                                       >  2:683235/1‑140 (MQ=255)

                                        tggcATTTTTGCCAGCCAGTCC‑TATAACTATCCCCTGATTTTTGATCCGAAAAGATGAATTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCg                                                                                     <  1:1048607/140‑1 (MQ=255)

                                             tttttGTCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCg                                                                                 <  2:634579/140‑1 (MQ=255)

                                              ttttGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGc                                                                                <  1:1493418/140‑1 (MQ=255)

                                                     agccagTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTg                                                                         <  2:1252188/140‑1 (MQ=255)

                                                       ccagTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGAc                                                                       <  1:161548/140‑1 (MQ=255)

                                                         agTCCGTATAACTAGCCCCTGATTTTCGATCCTAAAAGATGAACTCAATCCATGAAGAATGATTCGTTCTGCCGAATTATTGCTGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGa                                                                     <  2:1058074/140‑1 (MQ=255)

                                                            ccGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAgg                                                                  <  2:889644/140‑1 (MQ=255)

                                                                     tATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGTTTGACGAAGGGAATACCGt                                                         <  1:1608269/140‑1 (MQ=255)

                                                                     tATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGt                                                         >  2:718582/1‑140 (MQ=255)

                                                                       tCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGc                                                       >  2:807768/1‑140 (MQ=255)

                                                                       tCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGc                                                       >  2:797931/1‑140 (MQ=255)

                                                                       tCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGc                                                       >  2:510632/1‑140 (MQ=255)

                                                                         cccTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCg                                                     >  2:1289867/1‑140 (MQ=255)

                                                                         cccTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCg                                                     >  2:1417162/1‑140 (MQ=255)

                                                                         cccTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCg                                                     >  1:274847/1‑140 (MQ=255)

                                                                         cccTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCg                                                     >  1:825682/1‑140 (MQ=255)

                                                                            tGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCGttt                                                  >  1:1303450/1‑140 (MQ=255)

                                                                             gATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCGTTTa                                                 <  2:1399823/140‑1 (MQ=255)

                                                                                        cGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCGTTTATCGCACGTTAt                                      >  2:157175/1‑140 (MQ=255)

                                                                                           aaaGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCGTTTATCGCACGTTATCGt                                   <  1:72565/140‑1 (MQ=255)

                                                                                           aaaGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCGTTTATCGCACGTTATCGt                                   <  1:416627/140‑1 (MQ=255)

                                                                                           aaaGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCGTTTATCGCACGTTATCGt                                   <  2:254589/140‑1 (MQ=255)

                                                                                                    cTCAAACCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTAGCCTGCTTGACGAAGGGAATACCGTGCCGTTTATCGCACGTTATCGTAAGGAAATc                          >  2:1064544/1‑140 (MQ=255)

                                                                                                        aaCCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCGTTTATCGCACGTTATCGTAAGGAAATCACcg                      <  1:718582/140‑1 (MQ=255)

                                                                                                        aaCCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCGTTTATCGCACGTTATCGTAAGGAAATCACcg                      <  1:683235/140‑1 (MQ=255)

                                                                                                         aCCATGATGAATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCGTTTATCGCACGTTATCGTAAGGAAATCACcgg                     <  1:971616/140‑1 (MQ=255)

                                                                                                             tgatgaATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCGTTTATCGCACGTTATCGTAAGGAAATCACCGGCGGt                 >  2:1417757/1‑140 (MQ=255)

                                                                                                             tgatgaATGATTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCGTTTATCGCACGTTATCGTAAGGAAATCACCGGCGGt                 >  2:919412/1‑140 (MQ=255)

                                                                                                                 gaatgaTTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCGTTTATCGCACGTTATCGTAAGGAAATCACcg                      <  2:115522/131‑1 (MQ=255)

                                                                                                                 gaatgaTTCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCGTTTATCGCACGTTATCGTAAGGAAATCACcg                      >  1:115522/1‑131 (MQ=255)

                                                                                                                        tCGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCGTTTATCGCACGTTATCGTAAGGAAATCACCGGCGGTCTGGATGacac      >  2:1069053/1‑140 (MQ=255)

                                                                                                                         cGTTCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCGTTTATCGCACGTTATCGTAAGGAAATCACCGGCGGTCTGGATGACACg     <  1:1243608/140‑1 (MQ=255)

                                                                                                                           ttCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCGTTTATCGCACGTTATCGTAAGGAAATCACCGGCGGTCTGGATGACACGCa   <  1:1232847/140‑1 (MQ=255)

                                                                                                                            tCTGCCGAATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCGTTTATCGCACGTTATCGTAAGGAAATCACCGGCGGTCTGGATGACACGCAg  >  2:1236777/1‑140 (MQ=255)

GTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTGACGAAGGGAATACCGTGCCGTTTATCGCACGTTATCGTAAGGAAATCACCGGCGGTCTGGATGACACGCAG  >  NC_000913/3537277‑3537540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A