Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F73 I1 R1 27 211.6 7358776 99.3% 7307264 139.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,771,593 C→A S51I (AGC→ATC)  yibI ← DUF3302 domain‑containing protein YibI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,771,5930CA99.5% 652.9 / ‑4.2 192S51I (AGC→ATC) yibIDUF3302 domain‑containing protein YibI
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base A (115/75);  minor base T (1/0);  total (117/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.89e-01

Only 100 of 206 total aligned reads displayed.

GCGGCGATTTCCGGGTCAGTCGCTTTCTCCTGCGACGCAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑CTCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAA  >  NC_000913/3771464‑3771729
                                                                                                                                   |                                                                                                                                        
gcggcgATTTCCGGGTCAGTCGCTTTCTCCTGCGACGCAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGcc                                                                                                                                <  1:394541/140‑1 (MQ=255)
 cggcgATTTCCGGGTCAGTCGCTTTCTCCTGCGACGCAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGccc                                                                                                                               <  2:2564891/140‑1 (MQ=255)
 cggcgATTTCCGGGTCAGTCGCTTTCTCCTGCGACGCAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGccc                                                                                                                               <  1:1386641/140‑1 (MQ=255)
 cggcgATTTCCGGGTCAGTCGCTTTCTCCTGCGACGCAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGccc                                                                                                                               >  1:2017948/1‑140 (MQ=255)
   gcgATTTCCGGGTCAGTCGCTTTCTCCTGCGACGCAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAACCGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCccgc                                                                                                                             <  2:2807754/140‑1 (MQ=255)
   gcgATTTCCGGGTCAGTCGCTTTCTCCTGCGACGCAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCccgc                                                                                                                             <  2:1320580/140‑1 (MQ=255)
   gcgATTTCCGGGTCAGTCGCTTTCTCCTGCGACGCAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCccgc                                                                                                                             <  1:1376989/140‑1 (MQ=255)
   gcgATTTCCGGGTCAGTCGCTTTCTCCTGCGACGCAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCccgc                                                                                                                             <  2:3367984/140‑1 (MQ=255)
           cGGGTCAGTCGCTTTCTCCTGCGACGCAACATGTGACTGCATC‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGaa                                                                                                                     <  1:533901/140‑1 (MQ=255)
           cGGGTCAGTCGCTTTCTCCTGCGACGCAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGaa                                                                                                                     <  2:2864545/140‑1 (MQ=255)
                 aGTCGCTTTCTCCGGCGACGCAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGt                                                                                                               <  2:310808/140‑1 (MQ=255)
                  gTCGCTTTCTCCTGCGACGCAACATGTGACTGCATACCCCCAGCCACGCTCCGGTTGATAGAGCGCCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGt                                                                                                               <  2:2187085/140‑1 (MQ=255)
                  gTCGCTTTCTCCTGCGACGCAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTc                                                                                                              >  1:12677/1‑140 (MQ=255)
                   tCGCTTTCTCCTGCGACGCAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTAGGAATAGCGTCg                                                                                                             >  2:3627879/1‑140 (MQ=255)
                     gCTTTCTCCTGCGACGCAACATGTGACTGCATC‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGc                                                                                                           <  2:3166707/140‑1 (MQ=255)
                     gCTTTCTCCTGCGACGCAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGc                                                                                                           >  2:595568/1‑140 (MQ=255)
                     gCTTTCTCCTGCGACGCAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGc                                                                                                           <  2:2914374/140‑1 (MQ=255)
                     gCTTTCTCCTGCGACGCAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGc                                                                                                           <  1:2580245/140‑1 (MQ=255)
                            ccTGCGACGCAACATGTGATTGAATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGACCCACAGAACCGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGa                                                                                                    <  2:1684875/140‑1 (MQ=255)
                                 gACGCAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGtt                                                                                               >  1:1176478/1‑140 (MQ=255)
                                   cGCAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTgc                                                                                             >  2:1341571/1‑140 (MQ=255)
                                     cAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTgcgc                                                                                           >  1:2025767/1‑140 (MQ=255)
                                     cAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTgcgc                                                                                           >  1:1500929/1‑140 (MQ=255)
                                        cATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCttt                                                                                        <  1:3247044/140‑1 (MQ=255)
                                             gACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGc                                                                                   >  1:692083/1‑140 (MQ=255)
                                               cTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGGATTCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCa                                                                                  >  2:3407056/1‑140 (MQ=255)
                                               cTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCaa                                                                                 <  2:1500929/140‑1 (MQ=255)
                                               cTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCaa                                                                                 <  2:2025767/140‑1 (MQ=255)
                                                tGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAAt                                                                                <  2:12677/140‑1 (MQ=255)
                                                tGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAAt                                                                                <  1:893517/140‑1 (MQ=255)
                                                tGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCCCCCAGACCCCCAGAACCGGCCAGTTAACATGCGGAGTAAACGGG‑TTCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAAt                                                                                <  2:771170/140‑1 (MQ=255)
                                                  cATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCa                                                                              >  2:2358337/1‑140 (MQ=255)
                                                  cATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCa                                                                              >  1:2699068/1‑140 (MQ=255)
                                                  cATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCa                                                                              >  2:3667230/1‑140 (MQ=255)
                                                  cATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCa                                                                              >  2:1748710/1‑140 (MQ=255)
                                                  cATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCa                                                                              >  2:3015028/1‑140 (MQ=255)
                                                  cATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCa                                                                              >  1:668087/1‑140 (MQ=255)
                                                  cATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGAAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAAt                                                                                <  2:2323642/138‑1 (MQ=255)
                                                     a‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGAt                                                                           <  1:2618095/140‑1 (MQ=255)
                                                     a‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGAt                                                                           <  1:1341571/140‑1 (MQ=255)
                                                      cccccAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGACCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGAt                                                                           <  2:783947/139‑1 (MQ=255)
                                                       ccccAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATa                                                                          >  2:544712/1‑140 (MQ=255)
                                                        cccAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATaa                                                                         >  2:1156472/1‑140 (MQ=255)
                                                           aGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATACCATGCAGAGTAAACGGG‑ATCTCCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAggg                                                                      <  2:2231909/140‑1 (MQ=255)
                                                           aGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCCCCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAggg                                                                      <  1:2097087/140‑1 (MQ=255)
                                                           aGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAggg                                                                      >  2:3145445/1‑140 (MQ=255)
                                                           aGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAggg                                                                      <  1:2006373/140‑1 (MQ=255)
                                                           aGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTACGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAggg                                                                      >  2:135532/1‑140 (MQ=255)
                                                           aGCCACGCTCCGGTGGATAGAGCGTCGCCCAGACCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAggg                                                                      <  1:2138812/140‑1 (MQ=255)
                                                            gCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGa                                                                     >  2:324880/1‑140 (MQ=255)
                                                             ccACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAAt                                                                                >  2:2379415/1‑128 (MQ=255)
                                                             ccACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAAt                                                                                <  1:2379415/128‑1 (MQ=255)
                                                             ccACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGCAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGAt                                                                    >  2:2822970/1‑140 (MQ=255)
                                                             ccACGCCCCGGTTTAGAGAGCGTCGCCCAGGTCCACAAAAGCGGCCAGATAACATCGAGAGAAAAGAGG‑ATCAACCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGAt                                                                    <  2:2238375/140‑1 (MQ=255)
                                                                cGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTc                                                                 <  2:1875634/140‑1 (MQ=255)
                                                                  cTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCaa                                                                                 <  2:1080818/122‑1 (MQ=255)
                                                                  cTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCAt                                                               >  1:2446704/1‑140 (MQ=255)
                                                                  cTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCAt                                                               >  2:117162/1‑140 (MQ=255)
                                                                  cTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCAt                                                               >  1:2311002/1‑140 (MQ=255)
                                                                  cTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCAt                                                               >  2:398212/1‑140 (MQ=255)
                                                                       gTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATc                                                          >  1:999872/1‑140 (MQ=255)
                                                                           aTAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑CTCACCCAACCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCga                                                      >  1:3252806/1‑140 (MQ=255)
                                                                            tAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCgat                                                     <  2:2017948/140‑1 (MQ=255)
                                                                                 cGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTc                                                >  2:1759467/1‑140 (MQ=255)
                                                                                     gCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATa                                            >  1:2706629/1‑140 (MQ=255)
                                                                                     gCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATa                                            >  2:1575029/1‑140 (MQ=255)
                                                                                      cccAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATaa                                           >  2:2958624/1‑140 (MQ=255)
                                                                                           aTCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAAt                                      >  2:2438725/1‑140 (MQ=255)
                                                                                                   aaaCGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGaa                              <  1:3145445/140‑1 (MQ=255)
                                                                                                     aCGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAg                            >  1:101130/1‑140 (MQ=255)
                                                                                                     aCGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAg                            >  2:228461/1‑140 (MQ=255)
                                                                                                     aCGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAg                            >  1:966498/1‑140 (MQ=255)
                                                                                                     aCGGCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAg                            >  1:1551467/1‑140 (MQ=255)
                                                                                                       ggCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGAc                          >  2:1710985/1‑140 (MQ=255)
                                                                                                        gCCAGATAACATGCAGAGTAACCAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACg                         <  1:2822970/140‑1 (MQ=255)
                                                                                                        gCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAGAAAATCACCAGAAAGACg                         <  1:782390/140‑1 (MQ=255)
                                                                                                        gCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACg                         <  1:1079362/140‑1 (MQ=255)
                                                                                                        gCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACg                         <  1:1309363/140‑1 (MQ=255)
                                                                                                        gCCAGATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACg                         >  1:195452/1‑140 (MQ=255)
                                                                                                         ccAGATAACATGCAGAGTGAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGa                        >  1:2981162/1‑140 (MQ=255)
                                                                                                            gATAACATGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGa                     <  1:3624412/140‑1 (MQ=255)
                                                                                                                   tGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCAc              >  2:3613408/1‑140 (MQ=255)
                                                                                                                   tGCAGAGTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCAc              >  1:916361/1‑140 (MQ=255)
                                                                                                                       gagTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGGTAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCAATccc          >  2:3460935/1‑140 (MQ=255)
                                                                                                                       gagTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGa                           >  2:1381069/1‑123 (MQ=255)
                                                                                                                       gagTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTccc          >  1:1791083/1‑140 (MQ=255)
                                                                                                                       gagTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTccc          >  2:3252551/1‑140 (MQ=255)
                                                                                                                       gagTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTccc          >  2:333931/1‑140 (MQ=255)
                                                                                                                       gagTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTccc          >  1:1755837/1‑140 (MQ=255)
                                                                                                                       gagTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTccc          >  1:502666/1‑140 (MQ=255)
                                                                                                                       gagTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTccc          >  1:2763195/1‑140 (MQ=255)
                                                                                                                        agTAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCa         >  1:2873316/1‑140 (MQ=255)
                                                                                                                          tAAACAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAAc       >  1:2550725/1‑140 (MQ=255)
                                                                                                                           aaaCAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAAcg      <  1:333931/140‑1 (MQ=255)
                                                                                                                            aaCAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAg                 >  1:1115434/1‑128 (MQ=255)
                                                                                                                            aaCAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAAcgc     >  1:562074/1‑140 (MQ=255)
                                                                                                                             aCAGG‑ATCCCCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAAcgcg    <  2:1135480/140‑1 (MQ=255)
                                                                                                                             aCAGG‑ATCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAg                 >  1:3080629/1‑127 (MQ=255)
                                                                                                                             aCAGG‑AACACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAg                 <  2:3080629/127‑1 (MQ=255)
                                                                                                                                   cTCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGaa  >  1:442480/1‑137 (MQ=255)
                                                                                                                                   |                                                                                                                                        
GCGGCGATTTCCGGGTCAGTCGCTTTCTCCTGCGACGCAACATGTGACTGCATA‑CCCCAGCCACGCTCCGGTTGATAGAGCGTCGCCCAGATCCACAGAAACGGCCAGATAACATGCAGAGTAAACAGG‑CTCACCCAGCCCGCCGTATGAATAGCGTCGGCATGGGGATGGTTGCGCTTTTTGGCAATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAA  >  NC_000913/3771464‑3771729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A