Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F73 I1 R1	27	211.6	7358776	99.3%	7307264	139.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,868,172	C→A	E380* (GAA→TAA)	yidR ←	DUF3748 domain‑containing galacturonate catabolism protein YidR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,868,172	0	C	A	99.2%	471.7 / ‑3.3	133	E380* (GAA→TAA)	yidR	DUF3748 domain‑containing galacturonate catabolism protein YidR
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (70/62); total (70/63)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

Only 100 of 133 total aligned reads displayed.

CCGGCCAGAATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTG  >  NC_000913/3868037‑3868304

 cGGCCAGAATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTa                                                                                                                                 >  1:1028630/1‑140 (MQ=255)

 cGGCCAGAATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTa                                                                                                                                 <  1:1687789/140‑1 (MQ=255)

  ggCCAGAATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTaa                                                                                                                                >  1:2210099/1‑140 (MQ=255)

             cTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCt                                                                                                                     <  1:1769877/140‑1 (MQ=255)

                  aaCTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTcgccg                                                                                                                >  1:2628957/1‑140 (MQ=255)

                    cTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCt                                                                                                              <  2:155014/140‑1 (MQ=255)

                    cTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCt                                                                                                              <  1:3005721/140‑1 (MQ=255)

                    cTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGG‑TAAATACTCAACCTCGCCGCtt                                                                                                             >  2:2756375/1‑140 (MQ=255)

                     tGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCtt                                                                                                             >  2:2292272/1‑140 (MQ=255)

                         ttAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTgcg                                                                                                         >  1:929671/1‑140 (MQ=255)

                           aGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGCCCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCa                                                                                                       <  1:2292272/140‑1 (MQ=255)

                           aGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAGCCTCGCCGCTTTGCGCa                                                                                                       <  2:3524658/140‑1 (MQ=255)

                           aGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCa                                                                                                       <  1:3094011/140‑1 (MQ=255)

                           aGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCACAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCa                                                                                                       <  1:1794192/140‑1 (MQ=255)

                                tCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGc                                                                                                  <  1:1593499/140‑1 (MQ=255)

                                 cAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGca                                                                                                 >  2:3378587/1‑140 (MQ=255)

                                 cAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGca                                                                                                 >  1:2409230/1‑140 (MQ=255)

                                   gTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCTGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGcaca                                                                                               <  2:2794603/140‑1 (MQ=255)

                                   gTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGcaca                                                                                               >  1:3652588/1‑140 (MQ=255)

                                   gTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGcaca                                                                                               >  2:1764920/1‑140 (MQ=255)

                                     ttCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAg                                                                                             <  2:3187608/140‑1 (MQ=255)

                                      tCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGc                                                                                            >  1:1609649/1‑140 (MQ=255)

                                        ggTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCaa                                                                                          <  1:2490671/140‑1 (MQ=255)

                                         gTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAAt                                                                                         >  1:3578088/1‑140 (MQ=255)

                                         gTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAAt                                                                                         >  1:1210763/1‑140 (MQ=255)

                                            aTCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCg                                                                                      >  2:3255627/1‑140 (MQ=255)

                                            aTCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGGGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCg                                                                                      >  2:1061455/1‑140 (MQ=255)

                                             tCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGa                                                                                     <  2:1643497/140‑1 (MQ=255)

                                             tCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGa                                                                                     <  2:1792449/140‑1 (MQ=255)

                                              ccACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGAt                                                                                    >  2:728516/1‑140 (MQ=255)

                                              ccACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGAt                                                                                    >  1:2920469/1‑140 (MQ=255)

                                              ccACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGAt                                                                                    >  2:616579/1‑140 (MQ=255)

                                              ccACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGAt                                                                                    >  1:746877/1‑140 (MQ=255)

                                              ccACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGAt                                                                                    >  1:2487712/1‑140 (MQ=255)

                                              ccACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGCGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGAt                                                                                    >  1:1990233/1‑140 (MQ=255)

                                                 caGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTAt                                                                                 >  1:1079296/1‑140 (MQ=255)

                                                    cTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCa                                                                              <  2:1990233/140‑1 (MQ=255)

                                                      ggCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGc                                                                            >  2:2469436/1‑140 (MQ=255)

                                                       gccgccTTCCATCCACGCCAGCCTCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGca                                                                           >  1:574758/1‑140 (MQ=255)

                                                       gccgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGca                                                                           >  1:3676146/1‑140 (MQ=255)

                                                       gccgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGca                                                                           >  2:696993/1‑140 (MQ=255)

                                                       gccgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGca                                                                           >  1:350162/1‑140 (MQ=255)

                                                       gccgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGca                                                                           >  2:2092174/1‑140 (MQ=255)

                                                       gccgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGca                                                                           >  2:3339968/1‑140 (MQ=255)

                                                       gccgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGca                                                                           >  1:749939/1‑140 (MQ=255)

                                                       gccgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGca                                                                           >  1:1063202/1‑140 (MQ=255)

                                                       gccgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGca                                                                           >  1:2359535/1‑140 (MQ=255)

                                                       gccgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGca                                                                           >  2:3147179/1‑140 (MQ=255)

                                                       gccgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAACAATTCGATTATCCAGca                                                                           >  2:1815523/1‑140 (MQ=255)

                                                         cgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGa                                                                                     <  1:1853593/128‑1 (MQ=255)

                                                         cgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGa                                                                                     >  2:1853593/1‑128 (MQ=255)

                                                         cgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAACAATTCGATTATCCAGcaca                                                                         <  1:1815523/140‑1 (MQ=255)

                                                          gccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACaa                                                                        <  2:2442840/140‑1 (MQ=255)

                                                              tccatccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGcc                                                                    <  1:2901024/140‑1 (MQ=255)

                                                               ccatccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGccc                                                                   <  1:3163691/140‑1 (MQ=255)

                                                               ccatccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGccc                                                                   <  1:3147179/140‑1 (MQ=255)

                                                               ccatccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGccc                                                                   <  2:1160276/140‑1 (MQ=255)

                                                                  tccaCGCCAGCCACTGACCATCAGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAAc                                                                <  1:553001/140‑1 (MQ=255)

                                                                    caCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCa                                                              <  1:68920/140‑1 (MQ=255)

                                                                     aCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCAt                                                             >  2:116242/1‑140 (MQ=255)

                                                                     aCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCAt                                                             <  2:3652588/140‑1 (MQ=255)

                                                                     aCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCAt                                                             <  1:3339968/140‑1 (MQ=255)

                                                                      cGCCAGCCACTGACCATCCGGCGAGAAGACCGCGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCAtt                                                            <  1:2411885/140‑1 (MQ=255)

                                                                      cGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCAtt                                                            >  1:904941/1‑140 (MQ=255)

                                                                      cGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCAtt                                                            >  2:1932235/1‑140 (MQ=255)

                                                                          agccaCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTcc                                                        >  1:2816850/1‑140 (MQ=255)

                                                                           gccaCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCt                                                       <  2:2628957/140‑1 (MQ=255)

                                                                            ccaCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTg                                                      >  1:3178952/1‑140 (MQ=255)

                                                                            ccaCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTg                                                      >  1:2757947/1‑140 (MQ=255)

                                                                                  aCCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGAt                                                <  2:1991747/140‑1 (MQ=255)

                                                                                   ccATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATg                                               <  2:2829315/140‑1 (MQ=255)

                                                                                    cATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGc                                              <  2:1783580/140‑1 (MQ=255)

                                                                                    cATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGc                                              <  1:1875025/140‑1 (MQ=255)

                                                                                      tCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCa                                            >  2:336631/1‑140 (MQ=255)

                                                                                      tCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCa                                            >  2:3289556/1‑140 (MQ=255)

                                                                                      tCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCa                                            >  1:2289841/1‑140 (MQ=255)

                                                                                          gCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGG‑TAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTaa                                       <  1:2756375/140‑1 (MQ=255)

                                                                                           cGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTaa                                       >  1:1509647/1‑140 (MQ=255)

                                                                                              gaagaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATg                                    <  2:2920469/140‑1 (MQ=255)

                                                                                              gaagaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATg                                    <  2:1654045/140‑1 (MQ=255)

                                                                                                agaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCa                                  >  2:101490/1‑140 (MQ=255)

                                                                                                  aCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGa                                <  1:2256917/140‑1 (MQ=255)

                                                                                                  aCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGa                                <  2:746877/140‑1 (MQ=255)

                                                                                                   ccACTGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGAc                               <  1:3012868/140‑1 (MQ=255)

                                                                                                    cACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAg                                 >  1:465563/1‑137 (MQ=255)

                                                                                                    cACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAg                                 <  2:465563/137‑1 (MQ=255)

                                                                                                    cACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACt                              >  1:2289500/1‑140 (MQ=255)

                                                                                                       ggCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGaa                           >  1:3671447/1‑140 (MQ=255)

                                                                                                       ggCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGaa                           <  1:3542917/140‑1 (MQ=255)

                                                                                                         cGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAata                         >  2:2584675/1‑140 (MQ=255)

                                                                                                          gTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAatat                        <  2:891450/140‑1 (MQ=255)

                                                                                                           tCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACCGAATATc                       >  1:225516/1‑140 (MQ=255)

                                                                                                               cAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGtt                   <  1:3343881/140‑1 (MQ=255)

                                                                                                                   aGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGt               <  2:3178952/140‑1 (MQ=255)

                                                                                                                   aGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGt               <  1:3255627/140‑1 (MQ=255)

                                                                                                                    ggTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGtt              <  1:2584675/140‑1 (MQ=255)

                                                                                                                    ggTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGtt              <  1:2092174/140‑1 (MQ=255)

                                                                                                                     gTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTa             <  1:1764920/140‑1 (MQ=255)

                                                                                                                      tGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTAt            <  2:896791/140‑1 (MQ=255)

                                                                                                                       gAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATg           >  2:3540484/1‑140 (MQ=255)

CCGGCCAGAATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTG  >  NC_000913/3868037‑3868304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A