Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F73 I1 R1 28 161.3 5699896 99.3% 5659996 139.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,177,114 G→T T56N (ACT→AAT)  yqiB ← DUF1249 domain‑containing protein YqiB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,177,1140GT100.0% 478.2 / NA 136T56N (ACT→AAT) yqiBDUF1249 domain‑containing protein YqiB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (76/60);  total (76/60)

Only 100 of 147 total aligned reads displayed.

ACCGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAGGCGCAT  >  NC_000913/3176977‑3177247
                                                                                                                                         |                                                                                                                                     
 ccGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACTATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTAttgt                                                                                                                                    >  2:1465351/1‑140 (MQ=255)
 ccGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTAttgt                                                                                                                                    >  1:2159236/1‑140 (MQ=255)
 ccGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTAttgt                                                                                                                                    >  2:2092520/1‑140 (MQ=255)
 ccGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTAttgt                                                                                                                                    >  2:1914924/1‑140 (MQ=255)
   gcgcTTTGAAGCGAAAAATCTTCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTAt                                                                                                                                  >  2:1821108/1‑140 (MQ=255)
    cgcTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATc                                                                                                                                 >  1:2288953/1‑140 (MQ=255)
    cgcTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATc                                                                                                                                 >  1:1178525/1‑140 (MQ=255)
    cgcTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATc                                                                                                                                 >  2:2655069/1‑140 (MQ=255)
    cgcTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATc                                                                                                                                 >  2:2430285/1‑140 (MQ=255)
      cTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCgg                                                                                                                               >  2:1344164/1‑140 (MQ=255)
      cTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCgg                                                                                                                               >  2:2701992/1‑140 (MQ=255)
      cTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCgg                                                                                                                               >  2:1787901/1‑140 (MQ=255)
      cTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCgg                                                                                                                               >  2:2070679/1‑140 (MQ=255)
      cTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCgg                                                                                                                               >  1:1684709/1‑140 (MQ=255)
        ttGAAGCGAAAAATCTGCTGGCTTGAGCACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGt                                                                                                                             <  1:2614354/140‑1 (MQ=255)
        ttGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGt                                                                                                                             <  1:1375938/140‑1 (MQ=255)
        ttGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGt                                                                                                                             <  1:291888/140‑1 (MQ=255)
         tGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTc                                                                                                                            >  1:823746/1‑140 (MQ=255)
           aaGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGa                                                                                                                          <  1:1827262/140‑1 (MQ=255)
                      ctgctgGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCg                                                                                                               <  2:1003684/140‑1 (MQ=255)
                        gctgGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTc                                                                                                             <  1:1902188/140‑1 (MQ=255)
                        gctgGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTc                                                                                                             <  1:2655069/140‑1 (MQ=255)
                          tgGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAg                                                                                                           <  1:1500385/140‑1 (MQ=255)
                                      acTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGc                                                                                               >  2:1639680/1‑140 (MQ=255)
                                      acTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGc                                                                                               >  2:2730475/1‑140 (MQ=255)
                                      acTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGc                                                                                               >  2:1110785/1‑140 (MQ=255)
                                      acTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGc                                                                                               >  1:85126/1‑140 (MQ=255)
                                        ttCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCg                                                                                              >  1:1101561/1‑139 (MQ=255)
                                        ttCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCg                                                                                              <  2:1101561/139‑1 (MQ=255)
                                        ttCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCg                                                                                              <  2:835599/139‑1 (MQ=255)
                                           aGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTg                                                                                          >  1:1086656/1‑140 (MQ=255)
                                            gCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGc                                                                                         <  1:1245331/140‑1 (MQ=255)
                                              caccaTCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCa                                                                                       <  1:1598630/140‑1 (MQ=255)
                                              caccaTCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCa                                                                                       <  2:2568705/140‑1 (MQ=255)
                                                ccaTCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCAcc                                                                                     >  2:139378/1‑140 (MQ=255)
                                                ccaTCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCAcc                                                                                     >  1:2450384/1‑140 (MQ=255)
                                                  aTCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGc                                                                                         >  2:1202327/1‑134 (MQ=255)
                                                  aTCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTg                                                                                   <  1:1787901/140‑1 (MQ=255)
                                                  aTCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTg                                                                                   >  1:869656/1‑140 (MQ=255)
                                                   tCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGa                                                                                  <  1:2345976/140‑1 (MQ=255)
                                                   tCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGa                                                                                  <  2:1447619/140‑1 (MQ=255)
                                                   tCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGa                                                                                  <  2:82438/140‑1 (MQ=255)
                                                    cgcgTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGAt                                                                                 >  1:1655498/1‑140 (MQ=255)
                                                    cgcgTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGAt                                                                                 >  1:2642669/1‑140 (MQ=255)
                                                         cATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCtt                                                                            <  2:1086656/140‑1 (MQ=255)
                                                                  gACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACTAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCg                                                                   <  2:1178525/140‑1 (MQ=255)
                                                                  gACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCg                                                                   >  2:349456/1‑140 (MQ=255)
                                                                   aCGCACCGTCATTGACGTAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGCATTGTATCGGGACGATTCCACAATCGTCAGCCGCTATTGTGCGTTTGCCACCCGCTAGCTTACAGTTTCGc                                                                  >  2:973165/1‑140 (MQ=255)
                                                                   aCGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGc                                                                  >  1:41420/1‑140 (MQ=255)
                                                                   aCGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGc                                                                  >  2:691278/1‑140 (MQ=255)
                                                                   aCGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGc                                                                  >  1:2204177/1‑140 (MQ=255)
                                                                   aCGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGc                                                                  >  2:2264065/1‑140 (MQ=255)
                                                                    cGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGcc                                                                 <  1:691278/140‑1 (MQ=255)
                                                                     gCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCg                                                                >  2:310600/1‑140 (MQ=255)
                                                                     gCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCg                                                                >  2:2352916/1‑140 (MQ=255)
                                                                       aCCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCggg                                                              >  1:2524891/1‑140 (MQ=255)
                                                                       aCCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCggg                                                              >  2:721277/1‑140 (MQ=255)
                                                                        ccGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGt                                                             <  1:2070679/140‑1 (MQ=255)
                                                                         cGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTg                                                            >  2:1241861/1‑140 (MQ=255)
                                                                         cGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTg                                                            >  1:119530/1‑140 (MQ=255)
                                                                           tCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCg                                                          >  2:1807255/1‑140 (MQ=255)
                                                                               tGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCAt                                                      >  2:1810157/1‑140 (MQ=255)
                                                                                 aCGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTg                                                    <  1:1810157/140‑1 (MQ=255)
                                                                                 aCGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTg                                                    <  2:2288953/140‑1 (MQ=255)
                                                                                  cGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTgc                                                   >  2:2423579/1‑140 (MQ=255)
                                                                                     aaGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGcgcg                                                >  1:2793282/1‑140 (MQ=255)
                                                                                     aaGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGcgcg                                                >  2:1189101/1‑140 (MQ=255)
                                                                                     aaGGCTCCAGTAACTGATCGCGGGCACAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGTGTCATTGcgcg                                                >  1:1503123/1‑140 (MQ=255)
                                                                                      aGGCTCCAGTAACTGATCGCGGGCACAGTCTGATCTATTGTCACCAGGGTATTGTATCGGGGCCAGTGCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCCGGTGCGTCATTGTGCgg                                               >  2:872872/1‑140 (MQ=255)
                                                                                       ggCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGt                                              >  2:446493/1‑140 (MQ=255)
                                                                                           ccAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACa                                          >  2:2764249/1‑140 (MQ=255)
                                                                                            cAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACaa                                         >  1:2707975/1‑140 (MQ=255)
                                                                                                  cTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGgcg                                   <  1:1639680/140‑1 (MQ=255)
                                                                                                  cTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGgcg                                   <  1:812477/140‑1 (MQ=255)
                                                                                                    gATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCa                                 <  2:1684709/140‑1 (MQ=255)
                                                                                                      tCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAAt                               >  1:2070286/1‑140 (MQ=255)
                                                                                                      tCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAAt                               >  1:2752606/1‑140 (MQ=255)
                                                                                                       cgcgGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAAtt                              >  2:563097/1‑140 (MQ=255)
                                                                                                       cgcgGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAAtt                              >  2:165348/1‑140 (MQ=255)
                                                                                                       cgcgGGCGCAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAAtt                              >  2:312161/1‑140 (MQ=255)
                                                                                                            gcgcAGTCTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGaa                         <  2:2065058/140‑1 (MQ=255)
                                                                                                                   cTGTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCa                  <  1:1821108/140‑1 (MQ=255)
                                                                                                                     gTTCTATTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCAtc                <  1:2764249/140‑1 (MQ=255)
                                                                                                                          aTTGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGca           >  2:2464913/1‑140 (MQ=255)
                                                                                                                           ttGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGcac          <  1:2224611/140‑1 (MQ=255)
                                                                                                                            tGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGcaca         >  2:2250372/1‑140 (MQ=255)
                                                                                                                            tGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGcaca         >  2:2728152/1‑140 (MQ=255)
                                                                                                                            tGTCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGcaca         <  1:2181675/140‑1 (MQ=255)
                                                                                                                              tCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAgg       >  2:495427/1‑140 (MQ=255)
                                                                                                                              tCACCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAgg       >  1:1920613/1‑140 (MQ=255)
                                                                                                                                aCCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAGgcg     >  1:2829566/1‑140 (MQ=255)
                                                                                                                                aCCAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAGgcg     >  2:354592/1‑140 (MQ=255)
                                                                                                                                 ccAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAGgcgc    <  2:2159236/140‑1 (MQ=255)
                                                                                                                                  cAGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAGGCGca   >  2:1897081/1‑140 (MQ=255)
                                                                                                                                   aGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGca           >  1:58345/1‑131 (MQ=255)
                                                                                                                                   aGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGca           <  2:58345/131‑1 (MQ=255)
                                                                                                                                   aGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAGGCGcat  <  1:165348/140‑1 (MQ=255)
                                                                                                                                   aGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAGGCGcat  <  2:1037607/140‑1 (MQ=255)
                                                                                                                                   aGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAGGCGcat  <  1:2728152/140‑1 (MQ=255)
                                                                                                                                   aGGGTATTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAGGCGcat  >  1:1037607/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                     
ACCGCGCTTTGAAGCGAAAAATCTGCTGGCTTGAACACACTTCAGCCACCATCGCGTCATGATACAGACGCACCGTCATTGACGGAAGGCTCCAGTAACTGATCGCGGGCGCAGTCTGTTCTATTGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAGGCGCAT  >  NC_000913/3176977‑3177247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A