Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F73 I1 R1	30	54.0	1878228	99.2%	1863202	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,215,332	C→A	D48Y (GAC→TAC)	mug ←	stationary phase mismatch/uracil DNA glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,215,332	0	C	A	100.0%	190.6 / NA	54	D48Y (GAC→TAC)	mug	stationary phase mismatch/uracil DNA glycosylase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (28/26); total (28/26)

GCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGCCAAAATATCCT  >  NC_000913/3215200‑3215466

gCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAAc                                                                                                                                 <  2:919753/140‑1 (MQ=255)

gCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAAc                                                                                                                                 <  1:429117/140‑1 (MQ=255)

 cTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAAcc                                                                                                                                <  1:784844/140‑1 (MQ=255)

 cTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAAcc                                                                                                                                <  2:2299/140‑1 (MQ=255)

    aCGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCgg                                                                                                                             <  1:933082/140‑1 (MQ=255)

      gcccgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGcc                                                                                                                           <  2:144625/140‑1 (MQ=255)

         cgccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGa                                                                                                                        >  2:672463/1‑140 (MQ=255)

           ccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGata                                                                                                                      >  1:751863/1‑140 (MQ=255)

           ccTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGata                                                                                                                      >  1:644924/1‑140 (MQ=255)

           ccTGCGCGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGcc                                                                                                                           <  2:513499/135‑1 (MQ=255)

           ccTGCGCGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGcc                                                                                                                           >  1:513499/1‑135 (MQ=255)

              gCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGatatat                                                                                                                   <  1:672463/140‑1 (MQ=255)

                gtgtAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCa                                                                                                                 >  1:740019/1‑140 (MQ=255)

                gtgtAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCa                                                                                                                 <  1:938954/140‑1 (MQ=255)

                         cTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGAGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGa                                                                                                        >  1:415528/1‑140 (MQ=255)

                                   aCTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGc                                                                                              <  2:740019/140‑1 (MQ=255)

                                      tCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCgg                                                                                           >  1:882509/1‑140 (MQ=255)

                                      tCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCgg                                                                                           >  1:332983/1‑140 (MQ=255)

                                             cTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCa                                                                                    <  1:905623/140‑1 (MQ=255)

                                                 cACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAgg                                                                                >  2:303852/1‑140 (MQ=255)

                                                      ttGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCATAAGCGATTTGCCGGATGAGCAAAGGGaaaa                                                                           >  2:156227/1‑140 (MQ=255)

                                                      ttGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGaaaa                                                                           >  1:328975/1‑140 (MQ=255)

                                                      ttGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGaaaa                                                                           >  2:742596/1‑140 (MQ=255)

                                                           cGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGt                                                                      <  1:142220/140‑1 (MQ=255)

                                                                       ttGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGa                                                          <  1:286442/140‑1 (MQ=255)

                                                                       ttGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGa                                                          >  1:67496/1‑140 (MQ=255)

                                                                       ttGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGa                                                          <  2:167188/140‑1 (MQ=255)

                                                                        tGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGaa                                                         >  1:740949/1‑140 (MQ=255)

                                                                        tGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGaa                                                         >  2:722452/1‑140 (MQ=255)

                                                                        tGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGaa                                                         >  2:629215/1‑140 (MQ=255)

                                                                        tGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGaa                                                         >  2:244873/1‑140 (MQ=255)

                                                                        tGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGaa                                                         >  2:747517/1‑140 (MQ=255)

                                                                        tGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGaa                                                         >  2:824177/1‑140 (MQ=255)

                                                                        tGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGa                                                          >  1:37516/1‑139 (MQ=255)

                                                                        tGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGa                                                          <  2:37516/139‑1 (MQ=255)

                                                                           tGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGc                                                      <  2:328975/140‑1 (MQ=255)

                                                                             aCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCATAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGccc                                                    <  1:156227/140‑1 (MQ=255)

                                                                             aCGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGccc                                                    <  2:882509/140‑1 (MQ=255)

                                                                                ccACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGa                                                          >  2:728448/1‑131 (MQ=255)

                                                                                ccACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGa                                                          <  1:728448/131‑1 (MQ=255)

                                                                                              cagcagATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCaaa                                   >  2:551669/1‑140 (MQ=255)

                                                                                                 cagATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAaca                                <  2:316558/140‑1 (MQ=255)

                                                                                                  agATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAacac                               <  1:244873/140‑1 (MQ=255)

                                                                                                   gATGCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGGCCCGGCGGATGAAAGCTCAGGGTTGATACCGCAAAAcgcg                              <  2:933694/140‑1 (MQ=255)

                                                                                                      gCTGTGCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGAcc                           >  1:113189/1‑140 (MQ=255)

                                                                                                          tgCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTa                       <  1:303852/140‑1 (MQ=255)

                                                                                                          tgCCTCCTGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTa                       <  1:551669/140‑1 (MQ=255)

                                                                                                                ctGCGGCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTg                 >  2:885466/1‑140 (MQ=255)

                                                                                                                    ggCTTCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGc             >  1:376575/1‑140 (MQ=255)

                                                                                                                        tCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGCCaaa         <  1:451869/140‑1 (MQ=255)

                                                                                                                        tCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGCCaaa         >  2:147610/1‑140 (MQ=255)

                                                                                                                        tCAACTGACGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGCCaaa         >  1:874531/1‑140 (MQ=255)

                                                                                                                        tCAACTGACGGTAGGGAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGCCaaa         >  2:64206/1‑140 (MQ=255)

                                                                                                                               aCGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGCCAAAATATCCt  <  2:332983/140‑1 (MQ=255)

                                                                                                                               aCGGTAGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGCCAAAATATCCt  <  2:874531/140‑1 (MQ=255)

GCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGCTTCAACTGACGGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGCCAAAATATCCT  >  NC_000913/3215200‑3215466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A