Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F73 I1 R1 28 161.3 5699896 99.3% 5659996 139.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,013,373 C→A G107G (GGC→GGA)  metE → cobalamin‑independent homocysteine transmethylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,013,3730CA99.2% 456.9 / NA 129G107G (GGC→GGA) metEcobalamin‑independent homocysteine transmethylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (68/60);  minor base T (0/1);  total (68/61)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

GGCGATTTTGCCTGGTACGATCATGTACTGACCACCAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGCGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGCTGCTGGACGAAGTGGACGAGGCGCTGGCG  >  NC_000913/4013236‑4013508
                                                                                                                                         |                                                                                                                                       
        tGCCTGGTACGATCATGTACTGACCACCAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTgcgg                                                                                                                               >  1:1717937/1‑140 (MQ=255)
         gCCTGGTACGATCATGTACTGACCACCAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAACATGGTCCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTgcggc                                                                                                                              <  2:1006545/140‑1 (MQ=255)
                   aTCATGTACTGACCACCAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGaa                                                                                                                    >  2:1192064/1‑140 (MQ=255)
                    tCATGTACTGACCACCAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGaaa                                                                                                                   <  1:969178/140‑1 (MQ=255)
                        gTACTGACCACCAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGAc                                                                                                               >  1:1955519/1‑140 (MQ=255)
                         tACTGACCACCAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGAcc                                                                                                              >  2:1658408/1‑140 (MQ=255)
                         tACTGACCACCAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGAcc                                                                                                              >  2:2559070/1‑140 (MQ=255)
                         tACTGACCACCAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGAcc                                                                                                              >  2:2786528/1‑140 (MQ=255)
                            tGACCACCAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCaaa                                                                                                           <  2:1717937/140‑1 (MQ=255)
                            tGACCACCAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCaaa                                                                                                           <  2:2148072/140‑1 (MQ=255)
                             gACCACCAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAAt                                                                                                          >  1:1869610/1‑140 (MQ=255)
                             gACCACCAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAAt                                                                                                          >  2:706873/1‑140 (MQ=255)
                              accaccAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGGCACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCaaa                                                                                                           >  1:2228676/1‑138 (MQ=255)
                              accaccAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGGCACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCaaa                                                                                                           <  2:2228676/138‑1 (MQ=255)
                              accaccAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATg                                                                                                         >  1:2174287/1‑140 (MQ=255)
                              accaccAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATg                                                                                                         >  2:1887235/1‑140 (MQ=255)
                              accaccAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATg                                                                                                         >  1:1064510/1‑140 (MQ=255)
                              accaccAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATg                                                                                                         >  1:2342582/1‑140 (MQ=255)
                              accaccAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATg                                                                                                         >  1:737032/1‑140 (MQ=255)
                              accaccAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATg                                                                                                         >  2:2705300/1‑140 (MQ=255)
                              accaccAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATg                                                                                                         >  1:1166225/1‑140 (MQ=255)
                              accaccAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATg                                                                                                         >  1:1241004/1‑140 (MQ=255)
                              accaccAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCAGAAATGACCAGATg                                                                                                         >  1:281982/1‑140 (MQ=255)
                              accaccAGTCTGCTGCTGGGTAACGTTCCGACGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCAGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATg                                                                                                         >  2:682241/1‑140 (MQ=255)
                                caccaGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGt                                                                                                       >  1:1527597/1‑140 (MQ=255)
                                caccaGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGt                                                                                                       >  2:144428/1‑140 (MQ=255)
                                caccaGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGt                                                                                                       >  1:370955/1‑140 (MQ=255)
                                  ccaGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGttt                                                                                                     <  1:461568/140‑1 (MQ=255)
                                   caGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTa                                                                                                    >  2:861393/1‑140 (MQ=255)
                                     gTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAac                                                                                                  <  1:1267971/140‑1 (MQ=255)
                                      tCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAaca                                                                                                 <  2:493045/140‑1 (MQ=255)
                                       ctgctgctgGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAacac                                                                                                <  2:1527597/140‑1 (MQ=255)
                                        tgctgctgGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACAcc                                                                                               <  2:762480/140‑1 (MQ=255)
                                          ctgctgGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCa                                                                                              <  2:1528358/139‑1 (MQ=255)
                                           tgctgGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAAc                                                                                            <  1:861393/140‑1 (MQ=255)
                                           tgctgGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAAc                                                                                            <  2:1213811/140‑1 (MQ=255)
                                           tgctgGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAAc                                                                                            <  2:272370/140‑1 (MQ=255)
                                           tgctgGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAAc                                                                                            <  2:2438970/140‑1 (MQ=255)
                                           tgctgGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCAGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAAc                                                                                            >  1:2067920/1‑140 (MQ=255)
                                             ctgGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTa                                                                                          <  1:892428/140‑1 (MQ=255)
                                             ctgGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTa                                                                                          >  2:1166561/1‑140 (MQ=255)
                                              tgGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTAt                                                                                         <  2:1420174/140‑1 (MQ=255)
                                              tgGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTAt                                                                                         >  1:669500/1‑140 (MQ=255)
                                                 gTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCAc                                                                                      <  2:893291/140‑1 (MQ=255)
                                                  tAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACt                                                                                     >  1:1864415/1‑140 (MQ=255)
                                                  tAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACt                                                                                     >  1:1021138/1‑140 (MQ=255)
                                                     cGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACa                                                                                  <  2:672503/140‑1 (MQ=255)
                                                     cGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACa                                                                                  <  2:468055/140‑1 (MQ=255)
                                                     cGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACa                                                                                  <  2:2502649/140‑1 (MQ=255)
                                                     cGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACa                                                                                  <  1:878197/140‑1 (MQ=255)
                                                     cGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACa                                                                                  <  1:2705300/140‑1 (MQ=255)
                                                     cGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACa                                                                                  <  2:222674/140‑1 (MQ=255)
                                                     cGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACa                                                                                  <  2:1569672/140‑1 (MQ=255)
                                                     cGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACa                                                                                  <  2:2174287/140‑1 (MQ=255)
                                                       ttCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATg                                                                                >  2:2406877/1‑140 (MQ=255)
                                                       ttCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATg                                                                                >  1:1979992/1‑140 (MQ=255)
                                                            gcgcGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGcc                                                                           <  1:2786528/140‑1 (MQ=255)
                                                             cgcgTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCg                                                                          <  1:1750295/140‑1 (MQ=255)
                                                             cgcgTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCg                                                                          <  1:144428/140‑1 (MQ=255)
                                                                 tcatcaGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGt                                                                      <  1:706873/140‑1 (MQ=255)
                                                                 tcatcaGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGt                                                                      <  1:2269946/140‑1 (MQ=255)
                                                                     caGAACAAATATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGt                                                                  <  2:2774803/140‑1 (MQ=255)
                                                                     caGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGt                                                                  <  2:370955/140‑1 (MQ=255)
                                                                              gATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCa                                                         <  1:50646/140‑1 (MQ=255)
                                                                               aTGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCaa                                                        <  2:1869610/140‑1 (MQ=255)
                                                                               aTGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCaa                                                        <  1:1658408/140‑1 (MQ=255)
                                                                                  gTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAg                                                     >  1:508230/1‑140 (MQ=255)
                                                                                    tCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGtt                                                   >  1:1494199/1‑140 (MQ=255)
                                                                                        tAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGGGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCaaa                                               <  1:158372/140‑1 (MQ=255)
                                                                                        tAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCaaa                                               >  1:96645/1‑140 (MQ=255)
                                                                                        tAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCAGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTGCaaa                                               <  2:281982/140‑1 (MQ=255)
                                                                                                     ccTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTGTCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGc                                  >  2:2627428/1‑140 (MQ=255)
                                                                                                       tGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGa                                     <  1:1629855/135‑1 (MQ=255)
                                                                                                       tGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGa                                     >  2:1629855/1‑135 (MQ=255)
                                                                                                       tGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAg                                <  2:2206130/140‑1 (MQ=255)
                                                                                                       tGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAg                                <  1:1860922/140‑1 (MQ=255)
                                                                                                        gTTCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAgc                               >  1:1073788/1‑140 (MQ=255)
                                                                                                         ttCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAgct                              <  1:2662022/140‑1 (MQ=255)
                                                                                                         ttCCGTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAgct                              >  2:505397/1‑140 (MQ=255)
                                                                                                             gTATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGctgctg                          >  1:2420873/1‑140 (MQ=255)
                                                                                                              tATTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGCTGCTgg                         >  2:1394931/1‑140 (MQ=255)
                                                                                                               aTTGGTCGTGGACGTGCGCCGCCTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTGAAACTGTCCTGGACGCAGCTGCTGGa                        <  2:1923632/140‑1 (MQ=255)
                                                                                                               aTTGGTCGTGGACGTGCGCCGACTGGTGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGCTGCTGGa                        <  1:2158574/140‑1 (MQ=255)
                                                                                                               aTTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGCTGCTGGa                        >  2:1150/1‑140 (MQ=255)
                                                                                                               aTTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGCTGCTGGa                        <  2:2420873/140‑1 (MQ=255)
                                                                                                               aTTGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGCTGCTGGa                        <  2:1742236/140‑1 (MQ=255)
                                                                                                                ttGGTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGCTGCTGGAc                       >  1:2196407/1‑140 (MQ=255)
                                                                                                                   gTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGCTGCTGGACGaa                    >  2:760795/1‑140 (MQ=255)
                                                                                                                   gTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGCTGCTGGACGaa                    >  2:1698525/1‑140 (MQ=255)
                                                                                                                   gTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGCTGCTGGACGaa                    >  2:348157/1‑140 (MQ=255)
                                                                                                                   gTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGCTGCTGGACGaa                    >  1:1699104/1‑140 (MQ=255)
                                                                                                                   gTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGCTGCTGGACGaa                    >  2:1160767/1‑140 (MQ=255)
                                                                                                                   gTCGTGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGCTGCTGGACGaa                    >  1:377482/1‑140 (MQ=255)
                                                                                                                       tGGACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACCGACCTGGACGCAGCTGCTGGACGAAGTgg                >  2:1625591/1‑140 (MQ=255)
                                                                                                                        ggACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGCTGCTGGACGAAGTGGa               >  2:2176493/1‑140 (MQ=255)
                                                                                                                        ggACGTGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGCTGCTGGACGAAGTGGa               >  2:773979/1‑140 (MQ=255)
                                                                                                                             tGCGCCGACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGCTGCTGGACGAAGTGGACGAgg          >  2:286489/1‑140 (MQ=255)
                                                                                                                                   gACTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGCTGCTGGACGAAGTGGACGAggcgctgg    <  2:377482/140‑1 (MQ=255)
                                                                                                                                     cTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGCTGCTGGACGAAGTGGACGAggcgctggcg  <  1:2476108/140‑1 (MQ=255)
                                                                                                                                     cTGGAGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGCTGCTGGACGAAGTGGACGAggcgctggcg  <  1:760795/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
GGCGATTTTGCCTGGTACGATCATGTACTGACCACCAGTCTGCTGCTGGGTAACGTTCCGGCGCGTCATCAGAACAAAGATGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGGCGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCCAACAGTTCAAACTGACCTGGACGCAGCTGCTGGACGAAGTGGACGAGGCGCTGGCG  >  NC_000913/4013236‑4013508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A