Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F73 I1 R1	29	168.5	5953234	99.3%	5911561	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,730,681	G→T	P29T (CCC→ACC)	xylA ←	xylose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,730,681	0	G	T	99.1%	787.7 / ‑1.0	231	P29T (CCC→ACC)	xylA	xylose isomerase
Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (132/96); total (134/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.84e-01

Only 100 of 236 total aligned reads displayed.

CACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATA  >  NC_000913/3730546‑3730816

cACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTg                                                                                                                                     >  1:1430409/1‑140 (MQ=255)

cACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTg                                                                                                                                     >  2:675054/1‑140 (MQ=255)

cACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTg                                                                                                                                     >  2:2470223/1‑140 (MQ=255)

   cAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAg                                                                                                                                  <  1:2085189/140‑1 (MQ=255)

      gctgctGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGa                                                                                                                               <  2:1427781/140‑1 (MQ=255)

        tgctgcCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACg                                                                                                                             >  2:958677/1‑140 (MQ=255)

          ctgcCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGa                                                                                                                           >  1:428289/1‑140 (MQ=255)

                    cGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACgg                                                                                                                 >  2:2154863/1‑140 (MQ=255)

                       ttAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGa                                                                                                                           >  2:252582/1‑127 (MQ=255)

                       ttAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGa                                                                                                                           <  1:252582/127‑1 (MQ=255)

                          aaCGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGGGTGCCAGTAGCAGGCGGCAAAACGCAAGGGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTgg                                                                                                           >  2:2929716/1‑139 (MQ=255)

                                 ccACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGAtttt                                                                                                    >  2:1982446/1‑140 (MQ=255)

                                 ccACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGAtttt                                                                                                    >  1:301100/1‑140 (MQ=255)

                                    cacCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAg                                                                                                 <  2:1300749/140‑1 (MQ=255)

                                    cacCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAg                                                                                                 <  1:2556417/140‑1 (MQ=255)

                                         aaCATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAg                                                                                                 <  1:1206806/135‑1 (MQ=255)

                                         aaCATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAg                                                                                                 >  2:1206806/1‑135 (MQ=255)

                                         aaCATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTc                                                                                            >  2:2638901/1‑140 (MQ=255)

                                         aaCATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTc                                                                                            >  1:104711/1‑140 (MQ=255)

                                         aaCATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTc                                                                                            >  1:113707/1‑140 (MQ=255)

                                         aaCATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTc                                                                                            >  1:1937051/1‑140 (MQ=255)

                                           cATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGAttt                                                                                                     <  1:944701/129‑1 (MQ=255)

                                               tCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaacg                                                                                      >  2:857945/1‑140 (MQ=255)

                                               tCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaacg                                                                                      >  2:566743/1‑140 (MQ=255)

                                               tCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaacg                                                                                      >  2:431630/1‑140 (MQ=255)

                                               tCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaacg                                                                                      >  1:2821437/1‑140 (MQ=255)

                                               tCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaacg                                                                                      >  1:261523/1‑140 (MQ=255)

                                                 cGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaacgaa                                                                                    >  1:1273297/1‑140 (MQ=255)

                                                   ccccGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAg                                                                                                 >  1:1682871/1‑125 (MQ=255)

                                                   ccccGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaacgaacg                                                                                  >  2:1608347/1‑140 (MQ=255)

                                                   ccccGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaacgaacg                                                                                  >  1:967807/1‑140 (MQ=255)

                                                     ccGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAAcgcg                                                                                >  2:1357172/1‑140 (MQ=255)

                                                      cGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGa                                                                               <  1:566743/140‑1 (MQ=255)

                                                        ttCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATc                                                                             >  1:202114/1‑140 (MQ=255)

                                                        ttCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATc                                                                             >  1:2352719/1‑140 (MQ=255)

                                                        ttCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATc                                                                             >  1:1003782/1‑140 (MQ=255)

                                                           cagcagAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAg                                                                          <  1:1912828/140‑1 (MQ=255)

                                                                gAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGt                                                                     <  1:2470223/140‑1 (MQ=255)

                                                                gAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGt                                                                     <  1:340889/140‑1 (MQ=255)

                                                                 aaGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTc                                                                    >  1:784608/1‑140 (MQ=255)

                                                                  aGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCa                                                                   >  2:1109626/1‑140 (MQ=255)

                                                                  aGGTGTACCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCa                                                                   >  1:1534606/1‑140 (MQ=255)

                                                                      gtgCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAAt                                                               >  1:1858807/1‑140 (MQ=255)

                                                                       tgCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATa                                                              >  2:1090455/1‑140 (MQ=255)

                                                                       tgCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATa                                                              >  2:1746985/1‑140 (MQ=255)

                                                                       tgCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATa                                                              >  2:609819/1‑140 (MQ=255)

                                                                       tgCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATa                                                              <  1:1982446/140‑1 (MQ=255)

                                                                       tgCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATa                                                              <  1:2912570/140‑1 (MQ=255)

                                                                        gCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAg                                                             >  2:803681/1‑140 (MQ=255)

                                                                        gCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAg                                                             >  2:2545216/1‑140 (MQ=255)

                                                                         ccAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAgg                                                            <  2:1968201/140‑1 (MQ=255)

                                                                            gTAGCAGGCGGTAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCtt                                                         <  1:1461141/140‑1 (MQ=255)

                                                                            gTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCtt                                                         >  1:765268/1‑140 (MQ=255)

                                                                             tAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTg                                                        >  2:117017/1‑140 (MQ=255)

                                                                                   gcggcAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATAtt                                                  <  2:1855005/140‑1 (MQ=255)

                                                                                    cggcAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTg                                                 <  1:221806/140‑1 (MQ=255)

                                                                                    cggcAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTg                                                 <  1:1074539/140‑1 (MQ=255)

                                                                                     ggcAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGa                                                <  1:1639700/140‑1 (MQ=255)

                                                                                        aaaaCGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTTGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACt                                             <  2:2790378/140‑1 (MQ=255)

                                                                                          aaCGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAgg                                                            >  2:2372078/1‑123 (MQ=255)

                                                                                                aGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATc                                     <  2:2845059/140‑1 (MQ=255)

                                                                                                aGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATc                                     <  1:280664/140‑1 (MQ=255)

                                                                                                 gTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCa                                    >  1:887908/1‑140 (MQ=255)

                                                                                                           aTACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCcg                          <  2:91692/140‑1 (MQ=255)

                                                                                                           aTACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCcg                          <  1:1406465/140‑1 (MQ=255)

                                                                                                           aTACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCcg                          <  2:1574532/140‑1 (MQ=255)

                                                                                                           aTACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCcg                          <  2:113707/140‑1 (MQ=255)

                                                                                                              cGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCgg                       <  2:2583554/140‑1 (MQ=255)

                                                                                                              cGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCgg                       <  2:2332681/140‑1 (MQ=255)

                                                                                                               gCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCggg                      >  2:1017259/1‑140 (MQ=255)

                                                                                                                 ttACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCggg                      >  1:600191/1‑138 (MQ=255)

                                                                                                                 ttACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTg                    >  1:546942/1‑140 (MQ=255)

                                                                                                                   aCCCAGCACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTgat                  >  2:2974346/1‑140 (MQ=255)

                                                                                                                   aCCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTgat                  >  2:755087/1‑140 (MQ=255)

                                                                                                                   aCCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTgat                  >  1:2190442/1‑140 (MQ=255)

                                                                                                                   aCCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTgat                  >  2:778213/1‑140 (MQ=255)

                                                                                                                   aCCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTgat                  >  2:1219332/1‑140 (MQ=255)

                                                                                                                   aCCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTgat                  >  2:2403676/1‑140 (MQ=255)

                                                                                                                   aCCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTgat                  >  1:1333190/1‑140 (MQ=255)

                                                                                                                   aCCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTgat                  >  2:1445197/1‑140 (MQ=255)

                                                                                                                   aCCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTgat                  >  1:2640963/1‑140 (MQ=255)

                                                                                                                   aCCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTgat                  >  1:2137999/1‑140 (MQ=255)

                                                                                                                   aCCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTgat                  >  2:275665/1‑140 (MQ=255)

                                                                                                                   aCCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCCTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTgat                  >  1:998538/1‑140 (MQ=255)

                                                                                                                   aCCCAACACCAGTTCGTCGGCATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGCCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTgat                  >  1:2499581/1‑140 (MQ=255)

                                                                                                                   aCCCAACACCAGGTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTgat                  >  1:1244271/1‑140 (MQ=255)

                                                                                                                      cAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTgatgga               <  2:503233/140‑1 (MQ=255)

                                                                                                                        acacCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTgatggatg             <  1:715382/140‑1 (MQ=255)

                                                                                                                         cacCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGTCGCGGGTGATGgatga            <  2:1701707/140‑1 (MQ=255)

                                                                                                                         cacCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGgatga            <  1:1828920/140‑1 (MQ=255)

                                                                                                                         cacCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGgatga            <  1:2766390/140‑1 (MQ=255)

                                                                                                                         cacCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGgatga            <  2:1881643/140‑1 (MQ=255)

                                                                                                                         cacCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGgatga            <  1:2154863/140‑1 (MQ=255)

                                                                                                                         cacCAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGACGCGGGTgatggatta            <  2:22511/140‑3 (MQ=255)

                                                                                                                            cAGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGt         <  1:241679/140‑1 (MQ=255)

                                                                                                                             aGTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTc        >  2:2080177/1‑140 (MQ=255)

                                                                                                                              gTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTga                   <  1:2264930/128‑1 (MQ=255)

                                                                                                                              gTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCg       <  2:1245813/140‑1 (MQ=255)

                                                                                                                              gTTCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCg       <  1:2898785/140‑1 (MQ=255)

                                                                                                                               ttCGTCGGTATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGt      >  2:330924/1‑140 (MQ=255)

CACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGATGATGTCGTAATA  >  NC_000913/3730546‑3730816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A