Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F73 I1 R1	29	73.2	2528136	99.3%	2510439	139.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,323,958	G→T	A38E (GCG→GAG)	glmM ←	phosphoglucosamine mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,323,958	0	G	T	100.0%	187.0 / NA	52	A38E (GCG→GAG)	glmM	phosphoglucosamine mutase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (33/19); total (33/19)

GCCGGTGTTGGCATCGGGCCAGTGAAGAGTGCGGAAAGGCCCGCTGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGAAATATTTACGATTACTCATAGCGTTTGTTTTCCTTTGCAG  >  NC_000913/3323823‑3324091

gCCGGTGTTGGCATCGGGCCAGTGAAGAGTGCGGAAAGGCCCGCTGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAg                                                                                                                                   >  1:848745/1‑140 (MQ=255)

           cATCGGGCCAGTGAAGAGTGCGGAAAGGCCCGCTGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCcg                                                                                                                        <  2:29557/140‑1 (MQ=255)

               gggCCAGTGAAGAGTGCGGAAAGGCCCGCTGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGc                                                                                                                    >  2:709334/1‑140 (MQ=255)

                     gTGAAGAGTGCGGAAAGGCCCGCTGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAAcc                                                                                                              <  2:759005/140‑1 (MQ=255)

                           agTGCGGAAAGGCCCGCTGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAAAAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCtt                                                                                                        >  2:1127935/1‑140 (MQ=255)

                             tGCGGAAAGGCCCGCTGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTaa                                                                                                      >  1:680040/1‑140 (MQ=255)

                                ggAAAGGCCCGCTGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGca                                                                                                   >  2:397807/1‑140 (MQ=255)

                                      gCCCGCTGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAAt                                                                                             <  2:1234310/140‑1 (MQ=255)

                                      gCCCGCTGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAAt                                                                                             <  1:397807/140‑1 (MQ=255)

                                       cccGCTGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATc                                                                                            >  2:37106/1‑140 (MQ=255)

                                           cTGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGgt                                                                                        <  2:848745/140‑1 (MQ=255)

                                                gccAGACCCGCTTCCAGTGCTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGAt                                                                                   <  1:37106/140‑1 (MQ=255)

                                                     gcccGCTTCCAGTGCTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGgcg                                                                              <  1:682337/139‑1 (MQ=255)

                                                         gCTTCCAGTGCTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATc                                                                          <  1:187471/140‑1 (MQ=255)

                                                               aGTGCTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTac                                                                    >  1:1247248/1‑140 (MQ=255)

                                                                gTGCTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTaca                                                                   >  1:473225/1‑140 (MQ=255)

                                                                gTGCTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTaca                                                                   <  1:302479/140‑1 (MQ=255)

                                                                   cTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGa                                                                >  1:367348/1‑140 (MQ=255)

                                                                   cTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGa                                                                >  2:274952/1‑140 (MQ=255)

                                                                      aCTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCa                                                             >  1:632253/1‑140 (MQ=255)

                                                                      aCTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCa                                                             >  2:180591/1‑140 (MQ=255)

                                                                        tCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACg                                                           >  1:975808/1‑140 (MQ=255)

                                                                           aGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATTGGCGCATCCCCTACACGACCa                                                             >  1:532791/1‑135 (MQ=255)

                                                                           aGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATTGGCGCATCCCCTACACGACCa                                                             <  2:532791/135‑1 (MQ=255)

                                                                           aGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAAt                                                        <  2:975808/140‑1 (MQ=255)

                                                                                ataGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCAt                                                   <  1:274952/140‑1 (MQ=255)

                                                                                  aGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCg                                                 >  1:134583/1‑140 (MQ=255)

                                                                                  aGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCg                                                 >  1:1213282/1‑140 (MQ=255)

                                                                                       gAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTAcc                                            >  2:896563/1‑140 (MQ=255)

                                                                                        aaaTACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCg                                           <  2:680040/140‑1 (MQ=255)

                                                                                         aaTACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGa                                          >  2:602477/1‑140 (MQ=255)

                                                                                             cgcgTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGAAata                                      >  1:612479/1‑140 (MQ=255)

                                                                                              gcgTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGAAatat                                     >  2:1215304/1‑140 (MQ=255)

                                                                                               cgTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGAAATAtt                                    <  1:1128863/140‑1 (MQ=255)

                                                                                                 tgtCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGAAATATTTa                                  >  1:729928/1‑140 (MQ=255)

                                                                                                   tCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGAAATATTTACg                                <  1:896563/140‑1 (MQ=255)

                                                                                                          cAATAATAATCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGAAATATTTACGATTACTc                         <  2:72722/140‑1 (MQ=255)

                                                                                                            ataataatCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGAAATATTTACGATTACTCAt                       >  1:980548/1‑140 (MQ=255)

                                                                                                             taataatCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGAAATATTTACGATTACTCATa                      >  1:588803/1‑140 (MQ=255)

                                                                                                             taataatCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGAAATATTTACGATTACTCATa                      >  1:1125669/1‑140 (MQ=255)

                                                                                                              aataatCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGAAATATTTACGATTACTCATAg                     >  1:324069/1‑140 (MQ=255)

                                                                                                               ataatCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGTATCCCATCGGTACCGAAATATTTACGATTACTCATAGc                    <  1:602477/140‑1 (MQ=255)

                                                                                                               ataatCTTACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGAAATATTTACGATTACTCATAGc                    <  1:1127935/140‑1 (MQ=255)

                                                                                                                      tACGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGAAATATTTACGATTACTCATAGCgtttgtt             >  2:1209479/1‑140 (MQ=255)

                                                                                                                       aCGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGAAATATTTACGATTACTCATAGCgtttgttt            >  1:222223/1‑140 (MQ=255)

                                                                                                                        cGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGAAATATTTACGATTACTCATAGCGTTTGtttt           >  1:247512/1‑140 (MQ=255)

                                                                                                                        cGGGAGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGGACCGAAATATTTACGATTACTCATAGCGTTTGtttt           >  2:1149518/1‑140 (MQ=255)

                                                                                                                           gAGCCGTGGCGCTCCAGGACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGAAATATTTACGATTACTCATAGCGTTTGTTTTCCt        >  2:982798/1‑140 (MQ=255)

                                                                                                                            aGCCGTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGAAATATTTACGATTACTCATAGCGTTTGTTTTCCtt       >  1:563621/1‑140 (MQ=255)

                                                                                                                                gTGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGAAATATTTACGATTACTCATAGCGTTTGTTTTCCTTTGca   <  2:980548/140‑1 (MQ=255)

                                                                                                                                 tGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGAAATATTTACGATTACTCATAGCGTTTGTTTTCCTTTGcag  >  2:358373/1‑140 (MQ=255)

                                                                                                                                 tGGCGCTCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATACCATCGGTACCGAAATATTTACGATTACTCATAGCGTTTGTTTTCCTTTGcag  >  2:444325/1‑140 (MQ=255)

GCCGGTGTTGGCATCGGGCCAGTGAAGAGTGCGGAAAGGCCCGCTGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATATAGCCAGAAATACGCGTGTCTTTACCAATAATAATCTTACGGGAGCCGTGGCGCGCCAGCACTTTACCCGCGGCCCAACCCAGCTTAAGCACAAAATCAGGTGTGATCGGCGCATCCCCTACACGACCACGAATCCCATCGGTACCGAAATATTTACGATTACTCATAGCGTTTGTTTTCCTTTGCAG  >  NC_000913/3323823‑3324091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A