Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F73 I1 R1	22	108.2	3720756	99.3%	3694710	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,327,503	C→A	T54T (ACG→ACT)	rlmE ←	23S rRNA 2'‑O‑ribose U2552 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,327,503	0	C	A	100.0%	481.5 / NA	132	T54T (ACG→ACT)	rlmE	23S rRNA 2'‑O‑ribose U2552 methyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (65/67); total (65/67)

Only 100 of 132 total aligned reads displayed.

AAATCGCCCTGAAGAAAGTCCACACCAACGATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACCGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGCTAAAGTGTTCCTGAAGCCAGCGGCTGG  >  NC_000913/3327366‑3327636

aaaTCGCCCTGAAGAAAGTCCACACCAACGATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGt                                                                                                                                     >  1:901804/1‑140 (MQ=255)

      cccTGAAGAAAGTCCACACCAACGATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACATTATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTc                                                                                                                                <  1:1812254/140‑1 (MQ=255)

       ccTGAAGAAAGTCCACACCAACGATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTccc                                                                                                                              <  2:301336/140‑1 (MQ=255)

        cTGAAGAAAGTCCACACCAACGATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCg                                                                                                                             <  2:901804/140‑1 (MQ=255)

                     acacCAACGATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGttt                                                                                                                >  1:234721/1‑140 (MQ=255)

                     acacCAACGATAGGATCCATAGGTAAAAGATCGCAAGAGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGttt                                                                                                                >  1:1663630/1‑140 (MQ=255)

                         cAACGATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCa                                                                                                            <  1:1285021/140‑1 (MQ=255)

                            cGATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACtt                                                                                                         >  1:1770649/1‑140 (MQ=255)

                            cGATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACtt                                                                                                         >  2:1682030/1‑140 (MQ=255)

                               tAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGc                                                                                                      <  1:32010/140‑1 (MQ=255)

                                aGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCt                                                                                                     >  1:1278132/1‑140 (MQ=255)

                                 ggATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTg                                                                                                    >  1:692310/1‑140 (MQ=255)

                                   aTCCATAGGTAAAAGATCGCAAGGGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACt                                                                                                          <  2:18552/132‑1 (MQ=255)

                                   aTCCATAGGTAAAAGATCGCAAGGGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACt                                                                                                          >  1:18552/1‑132 (MQ=255)

                                   aTCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCa                                                                                                            <  1:1146635/130‑1 (MQ=255)

                                   aTCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCa                                                                                                            >  2:1146635/1‑130 (MQ=255)

                                   aTCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACt                                                                                                          <  2:671435/132‑1 (MQ=255)

                                   aTCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTa                                                                                                  >  2:1047016/1‑140 (MQ=255)

                                   aTCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTa                                                                                                  >  2:8468/1‑140 (MQ=255)

                                       aTAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTtc                                                                                              >  1:1615046/1‑140 (MQ=255)

                                       aTAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTtc                                                                                              >  1:344543/1‑140 (MQ=255)

                                       aTAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTtc                                                                                              >  2:450046/1‑140 (MQ=255)

                                       aTAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTtc                                                                                              >  2:1303248/1‑140 (MQ=255)

                                        tAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCt                                                                                                     <  1:1770077/132‑1 (MQ=255)

                                        tAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCt                                                                                                     >  2:1770077/1‑132 (MQ=255)

                                          ggTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTtcatc                                                                                           <  2:1137540/140‑1 (MQ=255)

                                          ggTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTtcatc                                                                                           <  2:1760815/140‑1 (MQ=255)

                                          ggTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTtcatc                                                                                           <  2:1064082/140‑1 (MQ=255)

                                          ggTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTtcatc                                                                                           <  2:549633/140‑1 (MQ=255)

                                          ggTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTtcatc                                                                                           <  2:1278132/140‑1 (MQ=255)

                                          ggTAAAAGATCGCAAGAGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTtcatc                                                                                           <  2:1663630/140‑1 (MQ=255)

                                           gTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTtcatca                                                                                          >  2:373443/1‑140 (MQ=255)

                                            tAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCaa                                                                                         >  2:409464/1‑140 (MQ=255)

                                              aaaGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTtcatc                                                                                           >  1:381214/1‑136 (MQ=255)

                                              aaaGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTtcatc                                                                                           <  2:381214/136‑1 (MQ=255)

                                              aaaGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGt                                                                                       >  1:181942/1‑140 (MQ=255)

                                              aaaGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGt                                                                                       >  1:177645/1‑140 (MQ=255)

                                                    cGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTtcat                                                                                            >  1:995566/1‑129 (MQ=255)

                                                    cGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTtcat                                                                                            <  2:995566/129‑1 (MQ=255)

                                                    cGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAAc                                                                                 >  2:1043656/1‑140 (MQ=255)

                                                     gCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTtcatc                                                                                           >  1:622143/1‑129 (MQ=255)

                                                     gCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGtt                                                                                      <  1:1735611/134‑1 (MQ=255)

                                                     gCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAAcc                                                                                <  2:1615046/140‑1 (MQ=255)

                                                     gCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAAcc                                                                                <  2:1479801/140‑1 (MQ=255)

                                                     gCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAAcc                                                                                >  1:211213/1‑140 (MQ=255)

                                                     gCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAAcc                                                                                >  1:1479801/1‑140 (MQ=255)

                                                     gCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAAcc                                                                                >  1:1238783/1‑140 (MQ=255)

                                                        aGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAgg                                                                             <  1:1047016/140‑1 (MQ=255)

                                                                    gCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTa                                                                 <  1:770828/140‑1 (MQ=255)

                                                                      cTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAAc                                                               <  2:767485/140‑1 (MQ=255)

                                                                         tGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACggg                                                                       <  1:329982/129‑1 (MQ=255)

                                                                         tGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACggg                                                                       >  2:329982/1‑129 (MQ=255)

                                                                         tGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAAcccc                                                            >  1:466345/1‑140 (MQ=255)

                                                                         tGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAAcccc                                                            <  2:138215/140‑1 (MQ=255)

                                                                                aaTTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCt                                                     <  1:1043656/140‑1 (MQ=255)

                                                                                    tGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGc                                                 <  2:211213/140‑1 (MQ=255)

                                                                                    tGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGc                                                 <  1:1303248/140‑1 (MQ=255)

                                                                                    tGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGc                                                 <  2:1238783/140‑1 (MQ=255)

                                                                                      ggTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCt                                               <  1:522348/140‑1 (MQ=255)

                                                                                      ggTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCt                                               <  1:1357433/140‑1 (MQ=255)

                                                                                          aCCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTg                                           >  2:350835/1‑140 (MQ=255)

                                                                                          aCCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTg                                           >  2:462091/1‑140 (MQ=255)

                                                                                           ccACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGa                                          >  1:1093984/1‑140 (MQ=255)

                                                                                           ccACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGa                                          <  2:200198/140‑1 (MQ=255)

                                                                                           ccACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGa                                          <  1:462091/140‑1 (MQ=255)

                                                                                            caca‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGaa                                         <  1:8468/140‑1 (MQ=255)

                                                                                             aca‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAAc                                        <  2:177645/140‑1 (MQ=255)

                                                                                                   ttGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATAtt                                   >  1:55370/1‑140 (MQ=255)

                                                                                                   ttGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATAtt                                   <  2:547012/140‑1 (MQ=255)

                                                                                                   ttGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATAtt                                   >  1:719953/1‑140 (MQ=255)

                                                                                                   ttGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATAtt                                   <  1:450046/140‑1 (MQ=255)

                                                                                                     gtgACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTa                                 <  1:1682030/140‑1 (MQ=255)

                                                                                                     gtgACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTa                                 <  2:692310/140‑1 (MQ=255)

                                                                                                     gtgACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTa                                 <  2:181942/140‑1 (MQ=255)

                                                                                                      tgACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTAt                                >  2:46704/1‑140 (MQ=255)

                                                                                                      tgACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTAt                                >  1:1346479/1‑140 (MQ=255)

                                                                                                       gACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATc                               >  1:1010829/1‑140 (MQ=255)

                                                                                                       gACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATc                               >  2:1262338/1‑140 (MQ=255)

                                                                                                       gACCAACCACCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGG‑ACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCg                              >  2:99551/1‑140 (MQ=255)

                                                                                                          caaccaCCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTg                                              <  1:1545225/122‑1 (MQ=255)

                                                                                                          caaccaCCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTg                                              >  2:1545225/1‑122 (MQ=255)

                                                                                                          caaccaCCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGc                             <  2:165916/139‑1 (MQ=255)

                                                                                                          caaccaCCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGc                             >  1:165916/1‑139 (MQ=255)

                                                                                                          caaccaCCCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGCt                            <  2:1346479/140‑1 (MQ=255)

                                                                                                            accaccCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGCTaa                          >  1:710025/1‑140 (MQ=255)

                                                                                                            accaccCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGCTaa                          >  1:1374991/1‑140 (MQ=255)

                                                                                                            accaccCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGCTaa                          >  1:651699/1‑140 (MQ=255)

                                                                                                             ccaccCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGCTaaa                         >  2:1013085/1‑140 (MQ=255)

                                                                                                             ccaccCGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGCTaaa                         >  2:465303/1‑140 (MQ=255)

                                                                                                                cccGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGCTAAAgtg                      <  1:256180/140‑1 (MQ=255)

                                                                                                                cccGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGCTAAAg                        >  1:981104/1‑138 (MQ=255)

                                                                                                                cccGGAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGCTAAAg                        <  2:981104/138‑1 (MQ=255)

                                                                                                                   ggAGCAGCACCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGCTAAAGTGTTc                   <  1:465303/140‑1 (MQ=255)

                                                                                                                      gcagcaCCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGCTAAAGTGTTCCTg                <  2:1010829/140‑1 (MQ=255)

                                                                                                                           aCCAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGCTAAAGTGTTCCTGAAGcc           >  1:1242624/1‑140 (MQ=255)

                                                                                                                            ccAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGCTAAAGTGTTCCTGAAGCCa          <  1:171244/140‑1 (MQ=255)

                                                                                                                            ccAAGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGCTAAAGTGTTCCTGAAGCCa          <  2:898336/140‑1 (MQ=255)

                                                                                                                               aGGTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGCTAAAGTGTTCCTGAAGCCAGCg       >  2:1152215/1‑140 (MQ=255)

                                                                                                                                 gTCGACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGCTAAAGTGTTCCTGAAGCCAGCGGc     <  1:1262338/140‑1 (MQ=255)

                                                                                                                                    gACAACAGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGCTAAAGCGGTCCTGAAGCAAGCGGCTgg  >  1:190684/1‑140 (MQ=255)

AAATCGCCCTGAAGAAAGTCCACACCAACGATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACA‑TATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACCGTCATTCCCGGTTTAAAGAGTTTGTCACTTTGCTGTATTTCATCAAGTTTAAACCAGGCACGGGAACGTAACCCCTTTTTCTGTGCCTGTTGAACATATTTATCGCTAAAGTGTTCCTGAAGCCAGCGGCTGG  >  NC_000913/3327366‑3327636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A