Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F73 I1 R1	27	59.0	2035004	99.3%	2020758	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,057,232	C→A	A393A (GCG→GCT)	serA ←	phosphoglycerate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,057,232	0	C	A	100.0%	157.6 / NA	45	A393A (GCG→GCT)	serA	phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (19/26); total (19/26)

TGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAG  >  NC_000913/3057095‑3057360

tGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgagc                                                                                                                                <  2:419627/140‑1 (MQ=255)

    cAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCtttt                                                                                                                            >  1:708550/1‑140 (MQ=255)

     aaCGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCttttt                                                                                                                           >  1:517590/1‑140 (MQ=255)

         cgGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGc                                                                                                                       <  2:137354/140‑1 (MQ=255)

          ggCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCa                                                                                                                      <  2:313352/140‑1 (MQ=255)

          ggCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCa                                                                                                                      <  2:53871/140‑1 (MQ=255)

               cGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTc                                                                                                                 <  2:197376/140‑1 (MQ=255)

                     ggAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTtcgtc                                                                                                           <  1:986267/140‑1 (MQ=255)

                      gagaAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTtcgtcg                                                                                                          <  2:727975/140‑1 (MQ=255)

                         aaGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCt                                                                                                       <  2:708550/140‑1 (MQ=255)

                          aGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCtt                                                                                                      >  1:672750/1‑140 (MQ=255)

                           gggATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTtc                                                                                                     <  2:517590/140‑1 (MQ=255)

                            ggATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCt                                                                                                       >  2:452812/1‑137 (MQ=255)

                            ggATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCt                                                                                                       <  1:452812/137‑1 (MQ=255)

                            ggATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTtca                                                                                                    >  1:927164/1‑140 (MQ=255)

                            ggATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTtca                                                                                                    >  2:190879/1‑140 (MQ=255)

                             gATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTtcaa                                                                                                   <  2:222308/140‑1 (MQ=255)

                             gATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTtcaa                                                                                                   <  2:636701/140‑1 (MQ=255)

                              aTAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTtcgtc                                                                                                           <  1:641637/131‑1 (MQ=255)

                              aTAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTtcgtc                                                                                                           >  2:641637/1‑131 (MQ=255)

                               tAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCCGAGCTTTTTCGGCAACGTCTTCGTCGGCTtcaata                                                                                                 <  2:173593/140‑1 (MQ=255)

                                    aaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTtcaatatcaat                                                                                            <  2:992024/140‑1 (MQ=255)

                                    aaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTtcaatatcaat                                                                                            <  1:190879/140‑1 (MQ=255)

                                     aaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTtcaatatcaata                                                                                           <  2:927164/140‑1 (MQ=255)

                                         ggCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACca                                                                                       <  1:651782/140‑1 (MQ=255)

                                                                    ttCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCa                                                            <  2:787456/140‑1 (MQ=255)

                                                                                  aTTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCgcggcg                                              <  2:544568/140‑1 (MQ=255)

                                                                                       tACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGtt                                         >  2:670927/1‑140 (MQ=255)

                                                                                        aCAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTg                                        <  1:670927/140‑1 (MQ=255)

                                                                                        aCAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTg                                        <  2:531804/140‑1 (MQ=255)

                                                                                           gcagACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACg                                     >  2:187252/1‑140 (MQ=255)

                                                                                            cagACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGc                                    >  1:741203/1‑140 (MQ=255)

                                                                                             agACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGcc                                   <  1:573111/140‑1 (MQ=255)

                                                                                              gACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTg                                        >  2:936308/1‑134 (MQ=255)

                                                                                              gACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTg                                        <  1:936308/134‑1 (MQ=255)

                                                                                               aCGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCt                                 >  2:587210/1‑140 (MQ=255)

                                                                                                 gggCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGc                               >  1:65345/1‑140 (MQ=255)

                                                                                                    cgcgAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGtt                                         <  1:77988/127‑1 (MQ=255)

                                                                                                    cgcgAATGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGtt                                         >  2:77988/1‑127 (MQ=255)

                                                                                                        aaTGGTACCCGGAATAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGa                        <  1:187252/140‑1 (MQ=255)

                                                                                                                    aaTAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCa            >  2:717613/1‑140 (MQ=255)

                                                                                                                     aTAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAg           >  2:1015850/1‑140 (MQ=255)

                                                                                                                      tAGCTTTCATTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAgc          >  2:922922/1‑140 (MQ=255)

                                                                                                                              aTTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAg  >  1:518294/1‑140 (MQ=255)

                                                                                                                              aTTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAg  <  2:65345/140‑1 (MQ=255)

                                                                                                                              aTTGCCTGCAGAGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAg  >  1:572883/1‑140 (MQ=255)

TGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAG  >  NC_000913/3057095‑3057360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A