Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F73 I1 R1	27	59.0	2035004	99.3%	2020758	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,303,260	G→T	D258Y (GAC→TAC)	yhbV →	putative peptidase YhbV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,303,260	0	G	T	98.7%	281.9 / ‑3.8	79	D258Y (GAC→TAC)	yhbV	putative peptidase YhbV
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (46/32); minor base A (1/0); total (47/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

TTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCGACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGCCTAAGTAAATAGCTCACTTTGTTAACAACTT  >  NC_000913/3303131‑3303394

tttGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCcg                                                                                                                              >  2:571649/1‑140 (MQ=255)

 ttGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCcgc                                                                                                                             >  2:656727/1‑140 (MQ=255)

          aTGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGaa                                                                                                                    >  2:362279/1‑140 (MQ=255)

                     aCCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGc                                                                                                         >  1:198856/1‑140 (MQ=255)

                      ccATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGca                                                                                                        <  2:554215/140‑1 (MQ=255)

                       cATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGcac                                                                                                       >  1:234124/1‑140 (MQ=255)

                         tGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGcacca                                                                                                     <  2:344487/140‑1 (MQ=255)

                          gAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGcaccac                                                                                                    >  2:143485/1‑140 (MQ=255)

                          gAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGcaccac                                                                                                    >  2:901986/1‑140 (MQ=255)

                           aGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACt                                                                                                   >  2:680129/1‑140 (MQ=255)

                           aGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACt                                                                                                   >  2:416875/1‑140 (MQ=255)

                             cGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGc                                                                                                 >  2:993319/1‑140 (MQ=255)

                               gCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTg                                                                                                          <  2:927819/129‑1 (MQ=255)

                               gCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTg                                                                                                          >  1:927819/1‑129 (MQ=255)

                               gCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCg                                                                                               >  1:427579/1‑140 (MQ=255)

                               gCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCg                                                                                               >  1:46351/1‑140 (MQ=255)

                               gCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCg                                                                                               >  2:316694/1‑140 (MQ=255)

                                    gTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGAc                                                                                          <  1:416875/140‑1 (MQ=255)

                                     tttACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACg                                                                                         <  2:936254/140‑1 (MQ=255)

                                              tCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGc                                                                                <  2:677918/140‑1 (MQ=255)

                                              tCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGc                                                                                <  1:143485/140‑1 (MQ=255)

                                              tCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGc                                                                                >  1:677918/1‑140 (MQ=255)

                                               cGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCg                                                                               >  2:304837/1‑140 (MQ=255)

                                               cGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCg                                                                               >  2:552296/1‑140 (MQ=255)

                                               cGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCg                                                                               >  2:682592/1‑140 (MQ=255)

                                               cGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCg                                                                               >  1:917427/1‑140 (MQ=255)

                                                    aCGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGt                                                                          >  2:701401/1‑140 (MQ=255)

                                                      gAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTaa                                                                        <  1:901986/140‑1 (MQ=255)

                                                       aGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAAc                                                                       >  2:157235/1‑140 (MQ=255)

                                                            gCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTa                                                                  >  2:38199/1‑140 (MQ=255)

                                                            gCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTa                                                                  >  2:579704/1‑140 (MQ=255)

                                                                ccATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTgg                                                              >  2:275281/1‑140 (MQ=255)

                                                                 cATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTggc                                                             >  1:391618/1‑140 (MQ=255)

                                                                        ggCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCt                                                      >  1:363169/1‑140 (MQ=255)

                                                                          ccTGGTTGATGTGGTCCGCCTGTCACCGCGGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTgg                                                    >  1:901771/1‑140 (MQ=255)

                                                                          ccTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTgg                                                    >  2:626844/1‑140 (MQ=255)

                                                                          ccTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTgg                                                    >  2:571008/1‑140 (MQ=255)

                                                                          ccTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTgg                                                    >  2:223021/1‑140 (MQ=255)

                                                                           cTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGc                                                   <  1:579704/140‑1 (MQ=255)

                                                                              gTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAgg                                                >  1:578529/1‑140 (MQ=255)

                                                                              gTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAgg                                                >  2:193375/1‑140 (MQ=255)

                                                                                   tgtgGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTgg                                           >  1:267419/1‑140 (MQ=255)

                                                                                       gTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCt                                       >  1:647580/1‑140 (MQ=255)

                                                                                       gTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCt                                       >  2:600548/1‑140 (MQ=255)

                                                                                        tCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTg                                      <  1:275281/140‑1 (MQ=255)

                                                                                        tCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTg                                      >  2:94023/1‑140 (MQ=255)

                                                                                        tCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTg                                      <  2:449645/140‑1 (MQ=255)

                                                                                        tCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTg                                      >  2:505809/1‑140 (MQ=255)

                                                                                        tCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTg                                      >  2:43901/1‑140 (MQ=255)

                                                                                         ccGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGc                                     <  2:647580/140‑1 (MQ=255)

                                                                                          cGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCa                                    <  2:454921/140‑1 (MQ=255)

                                                                                            ccTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAg                                  <  1:701401/140‑1 (MQ=255)

                                                                                            ccTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAg                                  <  1:193375/140‑1 (MQ=255)

                                                                                            ccTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAg                                  >  1:778599/1‑140 (MQ=255)

                                                                                            ccTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAg                                  <  2:556877/140‑1 (MQ=255)

                                                                                            ccTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTAGCGGCGGCTGGCAGGACTGGAGCTGCAAg                                  <  1:316694/140‑1 (MQ=255)

                                                                                              tGTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGcc                                <  1:682592/140‑1 (MQ=255)

                                                                                               gTCACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGCCt                               <  2:834766/140‑1 (MQ=255)

                                                                                                 cACCGCAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGCCTaa                             <  1:993319/140‑1 (MQ=255)

                                                                                                      cAGGGTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGCCTAAGTaaa                        >  1:720552/1‑140 (MQ=255)

                                                                                                          gTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGCCTAAGt                           <  2:30829/133‑1 (MQ=255)

                                                                                                          gTACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGCCTAAGt                           >  1:30829/1‑133 (MQ=255)

                                                                                                           tACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCa                                    <  1:452176/123‑1 (MQ=255)

                                                                                                           tACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCa                                    >  2:452176/1‑123 (MQ=255)

                                                                                                           tACTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGCCTAAGTAAATAGCt                   <  1:43901/140‑1 (MQ=255)

                                                                                                            aCTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGCCTAAGTAAATAGCTc                  <  1:571649/140‑1 (MQ=255)

                                                                                                            aCTGACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGCCTAAGTAAATAGCTc                  <  2:903423/140‑1 (MQ=255)

                                                                                                               gACACTTTCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGCCTAAGTAAATAGCTCACt               <  1:157235/140‑1 (MQ=255)

                                                                                                                     ttCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGTTGGCAGGACTGGAGCTGCAAGCCTAAGTAAATAGCt                   <  2:40540/130‑1 (MQ=255)

                                                                                                                     ttCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGTTGGCAGGACTGGAGCTGCAAGCCTAAGTAAATAGCt                   >  1:40540/1‑130 (MQ=255)

                                                                                                                     ttCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGCCTAAGTAAATAGCTCACTTTGTTa         >  1:106082/1‑140 (MQ=255)

                                                                                                                     ttCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGCCTAAGTAAATAGCTCACTTTGTTa         >  2:742193/1‑140 (MQ=255)

                                                                                                                      tCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGCCTAAGTAAATAGCTCACTTTGTTaa        <  2:917427/140‑1 (MQ=255)

                                                                                                                      tCGCGATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGCCTAAGTAAATAGCTCACTTTGTTaa        <  1:505809/140‑1 (MQ=255)

                                                                                                                       cgcgATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGCCTAAGTAAATAGCTCACTTTGTTaac       >  2:478034/1‑140 (MQ=255)

                                                                                                                        gcgATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGCCTAAGTAAATAGCTCACTTTGTTaaca      <  2:778599/140‑1 (MQ=255)

                                                                                                                        gcgATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGCCTAAGTAAATAGCTCACTTTGTTaaca      <  1:600548/140‑1 (MQ=255)

                                                                                                                          gATGCTCTACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGCCTAAGTAAATAGCTCACTTTGTTaacaac    <  1:793150/140‑1 (MQ=255)

                                                                                                                            tGCTCAACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGCCTAAGTAAATAGCTCACTTTGTTAACAACtt  >  1:996721/1‑140 (MQ=255)

TTTGTACTCAATGGCATTCAGACCATGAGCGGCTACGTTTACAACCTCGGTAACGAGCTGGCATCCATGCAGGGCCTGGTTGATGTGGTCCGCCTGTCACCGCAGGGTACTGACACTTTCGCGATGCTCGACGCCTTCCGCGCTAATGAAAATGGCGCTGCACCACTGCCGTTGACGGCGAACAGCGACTGTAACGGCTACTGGCGGCGGCTGGCAGGACTGGAGCTGCAAGCCTAAGTAAATAGCTCACTTTGTTAACAACTT  >  NC_000913/3303131‑3303394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A