Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F73 I1 R1	27	59.0	2035004	99.3%	2020758	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,391,069	G→T	A147A (GCC→GCA)	rsgA ←	ribosome small subunit‑dependent GTPase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,391,069	0	G	T	97.8%	161.3 / ‑3.1	46	A147A (GCC→GCA)	rsgA	ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (23/22); total (24/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CTGGAAACCATCAATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATAGGTTTTACGCCGTCGTAGAAATCCGGACGCGTCAACACCGAGGTA  >  NC_000913/4390935‑4391201

cTGGAAACCATCAATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGccac                                                                                                                                 >  1:966944/1‑140 (MQ=255)

cTGGAAACCATCAATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGccac                                                                                                                                 <  1:909390/140‑1 (MQ=255)

   gAAACCATCAATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAg                                                                                                                              >  2:388950/1‑140 (MQ=255)

       ccATCAATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTaa                                                                                                                          <  2:592212/140‑1 (MQ=255)

         aTCAATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACg                                                                                                                        <  2:209666/140‑1 (MQ=255)

         aTCAATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACg                                                                                                                        <  2:708244/140‑1 (MQ=255)

           cAATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGt                                                                                                                      <  1:219868/140‑1 (MQ=255)

           cAATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGt                                                                                                                      <  2:456991/140‑1 (MQ=255)

             aTACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCg                                                                                                                    >  1:217372/1‑140 (MQ=255)

             aTACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCg                                                                                                                    <  2:885729/140‑1 (MQ=255)

             aTACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCg                                                                                                                    >  1:770199/1‑140 (MQ=255)

                               tGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGc                                                                                                  <  1:377355/140‑1 (MQ=255)

                                   gTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCg                                                                                              >  2:369818/1‑140 (MQ=255)

                                      gATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCa                                                                                           >  1:501717/1‑140 (MQ=255)

                                       atatCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCaa                                                                                          <  2:284630/140‑1 (MQ=255)

                                         atCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCaaaa                                                                                        >  2:870/1‑140 (MQ=255)

                                              tCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACAGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCg                                                                                   >  1:88858/1‑140 (MQ=255)

                                                tGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGga                                                                                 >  1:628879/1‑140 (MQ=255)

                                                tGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGga                                                                                 >  1:591898/1‑140 (MQ=255)

                                                  cTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGgaga                                                                               <  1:503069/140‑1 (MQ=255)

                                                  cTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGgaga                                                                               >  1:873464/1‑140 (MQ=255)

                                                            aaCGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAgacaatgacaat                                                                     <  1:434830/140‑1 (MQ=255)

                                                                 cATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGt                                                                <  2:501717/140‑1 (MQ=255)

                                                                   tGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCg                                                              >  1:927121/1‑140 (MQ=255)

                                                                     ccTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGAt                                                            >  2:180936/1‑140 (MQ=255)

                                                                               ccAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCa                                                  >  2:910884/1‑140 (MQ=255)

                                                                                 agcagGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAAt                                                <  1:388950/140‑1 (MQ=255)

                                                                                  gcagGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATa                                               >  1:432899/1‑140 (MQ=255)

                                                                                        cTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATAGGtttt                                         >  2:73892/1‑140 (MQ=255)

                                                                                           tCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATAGGTTTTACg                                      <  1:910884/140‑1 (MQ=255)

                                                                                                ttgAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATAGGTTTTACGCcgtc                                 >  1:949824/1‑140 (MQ=255)

                                                                                                ttgAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATAGGTTTTACGCcgtc                                 >  2:336672/1‑140 (MQ=255)

                                                                                                   aGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATAGGTTTTACg                                      <  2:561099/132‑1 (MQ=255)

                                                                                                   aGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATAGGTTTTACg                                      >  1:561099/1‑132 (MQ=255)

                                                                                                   aGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATAGGTTTTACGCCGTCGTa                              <  1:870/140‑1 (MQ=255)

                                                                                                   aGCACAATAATCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACAGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATAGGTTTTACGCCGTCGTa                              <  2:88858/140‑1 (MQ=255)

                                                                                                          taatCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATAGGTTTTACGCCGTCGTAGAAATcc                       >  1:784179/1‑140 (MQ=255)

                                                                                                             tCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATAGGTTTTACGCCGTCGTAGAAATCCGGa                    <  1:369818/140‑1 (MQ=255)

                                                                                                             tCGGCTCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATAGGTTTTACGCCGTCGTAGAAATCCGGa                    <  1:180936/140‑1 (MQ=255)

                                                                                                                  tCAATCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATAGGTTTTACGCCGTCGTAGAAATCCGGACGCGt               >  2:875732/1‑140 (MQ=255)

                                                                                                                    aaTCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATAGGTTTTACGCCGTCGTAGAAATCCGGACGCGTCa             >  2:491988/1‑140 (MQ=255)

                                                                                                                    aaTCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATAGGTTTTACGCCGTCGTAGAAATCCGGACGCGTCa             >  2:64467/1‑140 (MQ=255)

                                                                                                                     aTCTGCAAGGTTTCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATAGGTTTTACGCCGTCGTAGAAATCCGGACGCGTCaa            <  1:336672/140‑1 (MQ=255)

                                                                                                                               tttCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATAGGTTTTACGCCGTCGTAGAAATCCGGACGCGTCAACACCGAGGTa  <  2:770199/140‑1 (MQ=255)

                                                                                                                                ttCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATAGGTTTTACGCCGTCGTAGAAATCCGGACGCGTCAACACCGAgg    <  2:140315/137‑1 (MQ=255)

                                                                                                                                ttCGCATGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATAGGTTTTACGCCGTCGTAGAAATCCGGACGCGTCAACACCGAgg    >  1:140315/1‑137 (MQ=255)

CTGGAAACCATCAATACGCGATAACCGATATTGCGGTAGATATCCATCTGCTCGTTGACGAACGCCATGCCTTCGTCGTCCAGCAGGTCTATCTTGTTGAGCACAATAATCGGCTCAATCTGCAAGGTTTCGCAGGCCACCAGGTAACGGTCGATAATATTGAGCGACAGCTCCGGCAAAATGGCGGAGACAATGACAATCTGGTCGATGTTGGCGGCAATAGGTTTTACGCCGTCGTAGAAATCCGGACGCGTCAACACCGAGGTA  >  NC_000913/4390935‑4391201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A