Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F73 I1 R1	27	59.0	2035004	99.3%	2020758	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,458,057	G→T	S33S (TCG→TCT)	pmbA →	metalloprotease PmbA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,458,057	0	G	T	100.0%	260.8 / NA	72	S33S (TCG→TCT)	pmbA	metalloprotease PmbA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (34/38); total (34/38)

CTCTGTTAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCGGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGATCACTGTTTATCACCAGAACCGCAAAGGTAGCG  >  NC_000913/4457927‑4458193

ctctGTTAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGCAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAGTCTGACGCTGCg                                                                                                                                 >  1:676493/1‑140 (MQ=255)

     ttAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGt                                                                                                                            >  2:458434/1‑140 (MQ=255)

             cAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGcc                                                                                                                        >  2:310557/1‑136 (MQ=255)

             cAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGcc                                                                                                                        <  1:310557/136‑1 (MQ=255)

             cAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCa                                                                                                                    >  1:32350/1‑140 (MQ=255)

               gagaAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGc                                                                                                                  <  1:42542/140‑1 (MQ=255)

                     ctctctACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGacc                                                                                                            >  2:795410/1‑140 (MQ=255)

                       ctctACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCg                                                                                                                       >  1:139392/1‑127 (MQ=255)

                       ctctACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCg                                                                                                                       <  2:139392/127‑1 (MQ=255)

                       ctctACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGa                                                                                                              >  2:406369/1‑136 (MQ=255)

                       ctctACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGa                                                                                                              <  1:406369/136‑1 (MQ=255)

                                  ggCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCg                                                                                               >  1:96357/1‑140 (MQ=255)

                                  ggCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCg                                                                                               >  1:783458/1‑140 (MQ=255)

                                  ggCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCg                                                                                               >  2:970473/1‑140 (MQ=255)

                                  ggCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCg                                                                                               >  2:32643/1‑140 (MQ=255)

                                      cTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAg                                                                                           >  1:781628/1‑140 (MQ=255)

                                      cTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAg                                                                                           >  2:458689/1‑140 (MQ=255)

                                      cTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAg                                                                                           >  1:95821/1‑140 (MQ=255)

                                      cTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAg                                                                                           >  1:821703/1‑140 (MQ=255)

                                      cTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAg                                                                                           >  2:332979/1‑140 (MQ=255)

                                      cTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAg                                                                                           >  1:445968/1‑140 (MQ=255)

                                      cTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCATAAg                                                                                           >  2:429498/1‑140 (MQ=255)

                                       ttGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGc                                                                                          >  2:779652/1‑140 (MQ=255)

                                       ttGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGc                                                                                          <  2:32350/140‑1 (MQ=255)

                                       ttGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGc                                                                                          <  2:5994/140‑1 (MQ=255)

                                       ttGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGc                                                                                          <  1:458434/140‑1 (MQ=255)

                                        tGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCa                                                                                         <  1:621808/140‑1 (MQ=255)

                                         gCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCa                                                                                         >  1:873801/1‑139 (MQ=255)

                                         gCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCa                                                                                         <  2:873801/139‑1 (MQ=255)

                                         gCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCAc                                                                                        <  1:795410/140‑1 (MQ=255)

                                         gCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCAc                                                                                        >  1:748866/1‑140 (MQ=255)

                                         gCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCAc                                                                                        <  2:445968/140‑1 (MQ=255)

                                          cAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCAcg                                                                                       <  1:292780/140‑1 (MQ=255)

                                          cAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCAcg                                                                                       <  1:154550/140‑1 (MQ=255)

                                             tGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGt                                                                                    >  1:958352/1‑140 (MQ=255)

                                                 aGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATg                                                                                <  2:119045/140‑1 (MQ=255)

                                                   tAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGt                                                                              <  2:783458/140‑1 (MQ=255)

                                                    aaTCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTg                                                                             <  1:195338/140‑1 (MQ=255)

                                                      tctctcAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGaa                                                                           <  1:458689/140‑1 (MQ=255)

                                                      tctctcAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGaa                                                                           <  1:332979/140‑1 (MQ=255)

                                                        tctcAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGt                                                                         <  1:322757/140‑1 (MQ=255)

                                                         ctcAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATg                                                                                >  1:339220/1‑132 (MQ=255)

                                                         ctcAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATg                                                                                <  2:339220/132‑1 (MQ=255)

                                                           cAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGga                                                                      >  1:965018/1‑140 (MQ=255)

                                                                tGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATg                                                                 <  2:821703/140‑1 (MQ=255)

                                                                  aaGCGCAGCGCAAGATTCTGGGAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGtt                                                               >  2:62046/1‑140 (MQ=255)

                                                                     cgcagcgcaAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGaa                                                            <  2:589362/140‑1 (MQ=255)

                                                                     cgcagcgcaAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGaa                                                            <  1:586264/140‑1 (MQ=255)

                                                                          cgcaAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCaa                                                       >  1:317737/1‑140 (MQ=255)

                                                                          cgcaAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCaa                                                       >  2:247817/1‑140 (MQ=255)

                                                                                      gaagaaGCAGTTTCCACTGCGCTGGAGTTGGCCTCTGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGgcgc                                           >  2:121819/1‑140 (MQ=255)

                                                                                       aagaagCAGTTTCCACTGCGCTGGAGTTGGCCTCTGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGgcgcg                                          <  1:121819/140‑1 (MQ=255)

                                                                                           agCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTgg                                      >  2:444024/1‑140 (MQ=255)

                                                                                            gCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTggg                                     <  2:958352/140‑1 (MQ=255)

                                                                                               gTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGAt                                  <  1:843638/140‑1 (MQ=255)

                                                                                               gTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGAt                                  <  2:757159/140‑1 (MQ=255)

                                                                                               gTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGAt                                  <  2:748866/140‑1 (MQ=255)

                                                                                               gTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGAt                                  <  2:47965/140‑1 (MQ=255)

                                                                                                 ttCCACTGCGCTGGAGTTGGCCTCTGGCGAGTCTGACGGAGCGGAAGTTGCCGTCAGCAAGACCCCCGACATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGCATGTTGAATTCAATAGCGATGGCGCGCTGGGGGTCa                                <  2:676493/140‑1 (MQ=255)

                                                                                                 ttCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGATCa                                <  2:96357/140‑1 (MQ=255)

                                                                                                 ttCCACTGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGATCa                                <  1:247817/140‑1 (MQ=255)

                                                                                                       tGCGCTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGATCACTGttt                          <  2:300115/140‑1 (MQ=255)

                                                                                                         cgcTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGATCACTGTTTAt                        >  1:905213/1‑140 (MQ=255)

                                                                                                          gcTGGAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGATCACTGTTTATc                       >  1:22822/1‑140 (MQ=255)

                                                                                                              gAGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGATCACTGTTTATCACCa                   >  2:536438/1‑140 (MQ=255)

                                                                                                               aGTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGATCACTGTTTATCACCAg                  <  2:997702/140‑1 (MQ=255)

                                                                                                                gTTGGCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGATCACTGTTTATCACCAGa                 <  1:970473/140‑1 (MQ=255)

                                                                                                                   ggCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGATCACTGTTTATCACCAGAAcc              >  1:685976/1‑140 (MQ=255)

                                                                                                                    gCCTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGATCACTGTTTATCACCAGAACCg             <  1:62046/140‑1 (MQ=255)

                                                                                                                     ccTCAGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGATCACTGTTTATCACCAGAACCGc            <  1:841325/140‑1 (MQ=255)

                                                                                                                         aGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGATCACTGTTTATCACCAGAACCGCaa          <  2:688191/138‑1 (MQ=255)

                                                                                                                         aGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGATCACTGTTTATCACCAGAACCGCaa          >  1:688191/1‑138 (MQ=255)

                                                                                                                         aGGCAAATCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGATCACTGTTTATCACCAGAACCGCAAAg        >  2:872403/1‑140 (MQ=255)

                                                                                                                               aTCTGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGATCACTGTTTATCACCAGAACCGCAAAGGTAgcg  <  2:905213/140‑1 (MQ=255)

CTCTGTTAGACTTCAGAGAAACTCTCTACATTATGGCACTTGCAATGAAAGTAATCTCTCAAGTTGAAGCGCAGCGCAAGATTCTGGAAGAAGCAGTTTCCACTGCGCTGGAGTTGGCCTCAGGCAAATCGGACGGTGCGGAAGTTGCCGTCAGCAAGACCACCGGCATTAGCGTAAGCACGCGTTATGGTGAAGTGGAGAATGTTGAATTCAATAGCGATGGCGCGCTGGGGATCACTGTTTATCACCAGAACCGCAAAGGTAGCG  >  NC_000913/4457927‑4458193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A