Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F73 I1 R1 30 54.0 1878228 99.2% 1863202 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,512,520 G→T A297E (GCA→GAA)  fecC ← ferric citrate ABC transporter membrane subunit FecC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,512,5200GT98.1% 185.7 / ‑2.5 53A297E (GCA→GAA) fecCferric citrate ABC transporter membrane subunit FecC
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (17/35);  total (18/35)
Fisher's exact test for biased strand distribution p-value = 3.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

GGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACG  >  NC_000913/4512384‑4512645
                                                                                                                                        |                                                                                                                             
gggTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCca                                                                                                                            >  2:118495/1‑140 (MQ=255)
 ggTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCcag                                                                                                                           <  1:310084/140‑1 (MQ=255)
     atgaAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCcag                                                                                                                           <  1:525618/136‑1 (MQ=255)
     atgaAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCcag                                                                                                                           >  2:525618/1‑136 (MQ=255)
     atgaAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCagcagc                                                                                                                       >  1:595034/1‑140 (MQ=255)
     atgaAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCagcagc                                                                                                                       >  2:118722/1‑140 (MQ=255)
     atgaAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCagcagc                                                                                                                       >  1:457707/1‑140 (MQ=255)
            tAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCg                                                                                                                <  1:77484/140‑1 (MQ=255)
            tAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCg                                                                                                                <  1:430537/140‑1 (MQ=255)
              aCCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTg                                                                                                              <  2:293553/140‑1 (MQ=255)
                   cgcAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGcccc                                                                                                         >  1:683643/1‑140 (MQ=255)
                    gcAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGccccc                                                                                                        >  1:766073/1‑140 (MQ=255)
                           tCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAgcagca                                                                                                 <  2:690388/140‑1 (MQ=255)
                               ccTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCt                                                                                             >  1:25488/1‑140 (MQ=255)
                               ccTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCt                                                                                             <  2:94326/140‑1 (MQ=255)
                                 tCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCa                                                                                           <  2:25488/140‑1 (MQ=255)
                                  cgtctcctcACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGAACTCCGGGGAAGGCCAGCGCGCGGGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCAc                                                                                          <  2:118268/137‑1 (MQ=255)
                                  cGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCAc                                                                                          >  1:770325/1‑140 (MQ=255)
                                     ctcctcACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTgg                                                                                       <  2:100733/140‑1 (MQ=255)
                                       cctcACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCa                                                                                     <  2:457707/140‑1 (MQ=255)
                                       cctcACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCa                                                                                     <  1:110468/140‑1 (MQ=255)
                                       cctcACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCa                                                                                     <  1:666585/140‑1 (MQ=255)
                                         tcACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGt                                                                                   <  2:652764/140‑1 (MQ=255)
                                                  aGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTgcgc                                                                          <  2:371483/140‑1 (MQ=255)
                                                  aGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTgcgc                                                                          <  1:880860/140‑1 (MQ=255)
                                                  aGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTgcgc                                                                          <  2:128456/140‑1 (MQ=255)
                                                   gACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCt                                                                         >  1:887368/1‑140 (MQ=255)
                                                       aaGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATc                                                                     <  1:900909/140‑1 (MQ=255)
                                                          cAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGaa                                                                  <  1:118495/140‑1 (MQ=255)
                                                          cAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGaa                                                                  >  2:204527/1‑140 (MQ=255)
                                                            aGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGc                                                                <  2:83536/140‑1 (MQ=255)
                                                            aGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGc                                                                <  2:766073/140‑1 (MQ=255)
                                                            aGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGc                                                                <  1:118722/140‑1 (MQ=255)
                                                            aGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGc                                                                <  2:335740/140‑1 (MQ=255)
                                                            aGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGc                                                                <  2:464386/140‑1 (MQ=255)
                                                            aGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATACTCACTGGCAGTACGTTGCGCTGATCGAAGc                                                                <  2:683643/140‑1 (MQ=255)
                                                             gggCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGcc                                                               <  1:492281/140‑1 (MQ=255)
                                                                       tCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAg                                                     <  1:204527/140‑1 (MQ=255)
                                                                        cAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAgc                                                    >  1:349044/1‑140 (MQ=255)
                                                                                gCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGa                                            <  2:595034/140‑1 (MQ=255)
                                                                                      cgcCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGc                                      >  2:695254/1‑140 (MQ=255)
                                                                                      cgcCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGc                                      >  1:757180/1‑140 (MQ=255)
                                                                                        ccTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCAc                                    >  1:108614/1‑140 (MQ=255)
                                                                                         cTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCAcc                                   <  2:887368/140‑1 (MQ=255)
                                                                                          tGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACca                                  >  2:654152/1‑140 (MQ=255)
                                                                                                cAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGAc                            <  2:818589/140‑1 (MQ=255)
                                                                                                             aaGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCAc               <  2:349044/140‑1 (MQ=255)
                                                                                                             aaGGCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCAc               >  1:9835/1‑140 (MQ=255)
                                                                                                                gCCAGCGCGCGTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCgg            <  2:798020/140‑1 (MQ=255)
                                                                                                                     cgcgcgTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTg       <  1:500669/140‑1 (MQ=255)
                                                                                                                          gTGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACg  <  2:9835/140‑1 (MQ=255)
                                                                                                                           tGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGAc          <  1:189478/131‑1 (MQ=255)
                                                                                                                           tGCGAGTACATCTTCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGAc          >  2:189478/1‑131 (MQ=255)
                                                                                                                                        |                                                                                                                             
GGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACG  >  NC_000913/4512384‑4512645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A