Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F73 I1 R1	23	178.8	6243766	99.3%	6200059	139.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,356,973	G→T	S750I (AGC→ATC)	gltB →	glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,356,973	0	G	T	98.6%	500.0 / 0.7	141	S750I (AGC→ATC)	gltB	glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (67/72); total (68/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

Only 100 of 141 total aligned reads displayed.

GGCATCAACAAAGGCTTGTACAAAATCATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTA  >  NC_000913/3356837‑3357108

  cATCAACAAAGGCTTGTACAAAATCATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCa                                                                                                                                    <  2:3038805/140‑1 (MQ=255)

  cATCAACAAAGGCTTGTACAAAATCATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCa                                                                                                                                    <  1:2700441/140‑1 (MQ=255)

  cATCAACAAAGGCTTGTACAAAATCATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCa                                                                                                                                    >  1:2709437/1‑140 (MQ=255)

   aTCAACAAAGGCTTGTACAAAATCATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCg                                                                                                                                      <  2:2759625/137‑1 (MQ=255)

   aTCAACAAAGGCTTGTACAAAATCATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCg                                                                                                                                      >  1:2759625/1‑137 (MQ=255)

   aTCAACAAAGGCTTGTACAAAATCATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGCCATCCGCAt                                                                                                                                   >  2:1861716/1‑140 (MQ=255)

    tCAACAAAGGCTTGTACAAAATCATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCAtt                                                                                                                                  >  2:2178579/1‑140 (MQ=255)

          aaGGCTTGTACAAAATCATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGa                                                                                                                            >  2:652878/1‑140 (MQ=255)

            ggCTTGTACAAAATCATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGc                                                                                                                          <  2:3078369/140‑1 (MQ=255)

            ggCTTGTACAAAATCATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGc                                                                                                                          <  2:1981865/140‑1 (MQ=255)

            ggCTTGTACAAAATCATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGc                                                                                                                          <  1:2750570/140‑1 (MQ=255)

                  tACAAAATCATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCtt                                                                                                                    >  2:1419548/1‑140 (MQ=255)

                    cAAAATCATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTg                                                                                                                  <  1:566715/140‑1 (MQ=255)

                      aaaTCATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGaa                                                                                                                <  1:3015893/140‑1 (MQ=255)

                       aaTCATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAg                                                                                                               <  1:224605/140‑1 (MQ=255)

                       aaTCATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAg                                                                                                               >  1:2507652/1‑140 (MQ=255)

                         tCATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGAc                                                                                                             <  1:2234269/140‑1 (MQ=255)

                          cATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACt                                                                                                            <  1:2048631/140‑1 (MQ=255)

                           aTGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACtt                                                                                                           <  2:1569013/140‑1 (MQ=255)

                            tGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTc                                                                                                          >  1:9020/1‑140 (MQ=255)

                            tGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTc                                                                                                          >  1:1114061/1‑140 (MQ=255)

                            tGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCACTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGaa                                                                                                                <  1:1074063/134‑1 (MQ=255)

                                cAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCcagc                                                                                                      <  1:2366003/140‑1 (MQ=255)

                                cAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCcagc                                                                                                      <  2:1760167/140‑1 (MQ=255)

                                  aaaTGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCcagcag                                                                                                    <  1:1911699/140‑1 (MQ=255)

                                   aaTGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAgg                                                                                                   <  2:376501/140‑1 (MQ=255)

                                   aaTGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAgg                                                                                                   <  2:1467599/140‑1 (MQ=255)

                                    aTGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGa                                                                                                  <  1:767523/140‑1 (MQ=255)

                                    aTGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGa                                                                                                  <  1:1381894/140‑1 (MQ=255)

                                         cATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATctgc                                                                                             >  2:643203/1‑140 (MQ=255)

                                          aTCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATctgct                                                                                            <  1:546153/140‑1 (MQ=255)

                                            ctcCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGa                                                                                          <  2:1398565/140‑1 (MQ=255)

                                              ccaccaTCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAAt                                                                                        <  2:1027156/140‑1 (MQ=255)

                                               caccaTCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATc                                                                                       >  1:1989408/1‑140 (MQ=255)

                                                   aTCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTc                                                                                   <  2:1114061/140‑1 (MQ=255)

                                                     cGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGa                                                                                 >  1:167807/1‑140 (MQ=255)

                                                       ccTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGaaa                                                                               <  2:350998/140‑1 (MQ=255)

                                                       ccTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGaaa                                                                               >  2:1128158/1‑140 (MQ=255)

                                                       ccTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGaaa                                                                               <  1:2298422/140‑1 (MQ=255)

                                                                  tgctCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCctggct                                                                    >  2:2650375/1‑140 (MQ=255)

                                                                   gctCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCctggctg                                                                   <  1:892242/140‑1 (MQ=255)

                                                                    ctCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGaaa                                                                               <  1:1717920/127‑1 (MQ=255)

                                                                    ctCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGaaa                                                                               >  2:1717920/1‑127 (MQ=255)

                                                                    ctCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCctggctgg                                                                  >  2:2775589/1‑140 (MQ=255)

                                                                    ctCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCctggctgg                                                                  >  2:300201/1‑140 (MQ=255)

                                                                          aCTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTa                                                            <  1:1324154/140‑1 (MQ=255)

                                                                           cTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTaa                                                           <  1:2710628/140‑1 (MQ=255)

                                                                              tttGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGcc                                                        >  2:832588/1‑140 (MQ=255)

                                                                                   aGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCa                                                   >  2:639779/1‑140 (MQ=255)

                                                                                   aGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCa                                                   >  2:5630/1‑140 (MQ=255)

                                                                                     gggtcggtcTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGc                                                 <  2:1130990/139‑1 (MQ=255)

                                                                                     cggtcggtCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGc                                                 <  1:1419548/140‑1 (MQ=255)

                                                                                      ggtcggtcTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGcc                                                >  1:178973/1‑140 (MQ=255)

                                                                                        tcggtcTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAg                                              >  2:2558927/1‑140 (MQ=255)

                                                                                        tcggtcTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAg                                              >  1:2911970/1‑140 (MQ=255)

                                                                                         cggtcTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAgg                                             <  1:2110856/140‑1 (MQ=255)

                                                                                            tcTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCg                                          <  1:643203/140‑1 (MQ=255)

                                                                                              tACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGt                                        <  1:2281242/140‑1 (MQ=255)

                                                                                              tACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGt                                        <  1:2037898/140‑1 (MQ=255)

                                                                                               acacGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTc                                       >  1:1246638/1‑140 (MQ=255)

                                                                                                  cGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTctgc                                    >  1:3046159/1‑140 (MQ=255)

                                                                                                     tgatgTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGa                                 >  2:1496300/1‑140 (MQ=255)

                                                                                                      gatgTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGt                                        <  1:992616/132‑1 (MQ=255)

                                                                                                      gatgTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGaa                                >  2:1888709/1‑140 (MQ=255)

                                                                                                      gatgTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGaa                                >  2:1526518/1‑140 (MQ=255)

                                                                                                        tgTAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAAt                              <  1:2988150/140‑1 (MQ=255)

                                                                                                          tAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAggg                                            >  2:1481473/1‑124 (MQ=255)

                                                                                                          tAGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATAc                            >  1:2915373/1‑140 (MQ=255)

                                                                                                           aGTGGGCCTGTGCTTCGAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACg                           >  2:1971733/1‑140 (MQ=255)

                                                                                                           aGTGGGCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCg                                          <  1:491377/125‑1 (MQ=255)

                                                                                                               ggCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCa                       >  1:1467226/1‑140 (MQ=255)

                                                                                                               ggCCTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCa                       <  2:184135/140‑1 (MQ=255)

                                                                                                               ggCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCa                       >  1:740552/1‑140 (MQ=255)

                                                                                                               ggCCTGTGCTTCCAGGAGGCGGTCATCCGCATTGGAGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGTCGCGTAAGCCCCTCAGCCAGGGCGGTCTGCTGAAATAGGTCCa                       >  2:2751099/1‑140 (MQ=255)

                                                                                                                 ccTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCAcg                     >  2:1650707/1‑140 (MQ=255)

                                                                                                                 ccTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCAcg                     >  1:2559529/1‑140 (MQ=255)

                                                                                                                 ccTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCAcg                     >  2:2591599/1‑140 (MQ=255)

                                                                                                                  cTGTGCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTct                                      >  1:2846757/1‑122 (MQ=255)

                                                                                                                    gtgCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCAcggcg                  >  2:2669666/1‑140 (MQ=255)

                                                                                                                    gtgCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCAcggcg                  >  1:488867/1‑140 (MQ=255)

                                                                                                                    gtgCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCAcggcg                  >  2:233485/1‑140 (MQ=255)

                                                                                                                    gtgCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCAcggcg                  >  1:772588/1‑140 (MQ=255)

                                                                                                                      gCTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACggcggc                <  2:967169/140‑1 (MQ=255)

                                                                                                                       cTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGgcggcg               <  2:1310760/140‑1 (MQ=255)

                                                                                                                       cTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGgcggcg               >  1:1020116/1‑140 (MQ=255)

                                                                                                                       cTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGgcggcg               <  2:1020116/140‑1 (MQ=255)

                                                                                                                       cTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGgcggcg               <  1:1321345/140‑1 (MQ=255)

                                                                                                                       cTTCCAGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGgcggcg               >  2:240794/1‑140 (MQ=255)

                                                                                                                            aGGGGGCGGTCATCCGCATTGGTGGATCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATAc          >  1:1271858/1‑140 (MQ=255)

                                                                                                                            aGGGGGCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATAc          <  2:57836/140‑1 (MQ=255)

                                                                                                                              ggggCGGTCATCCGCATTGGTGGATCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCa        <  2:1271858/140‑1 (MQ=255)

                                                                                                                              ggggCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCa        <  2:2911970/140‑1 (MQ=255)

                                                                                                                              ggggCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCa        <  1:1526518/140‑1 (MQ=255)

                                                                                                                               gggCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCAc       >  2:2146705/1‑140 (MQ=255)

                                                                                                                               gggCGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCAc       >  1:4335/1‑140 (MQ=255)

                                                                                                                                  cGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGcc    >  2:2480421/1‑140 (MQ=255)

                                                                                                                                  cGGTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGcc    >  1:3039789/1‑140 (MQ=255)

                                                                                                                                   ggTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCt   <  1:652878/140‑1 (MQ=255)

                                                                                                                                    gTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATAc          <  2:2927733/132‑1 (MQ=255)

                                                                                                                                    gTCATCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTa  <  1:1856410/140‑1 (MQ=255)

GGCATCAACAAAGGCTTGTACAAAATCATGTCCAAAATGGGCATCTCCACCATCGCCTCTTACCGCTGCTCGAAACTGTTTGAAGCGGTCGGTCTACACGATGATGTAGTGGGCCTGTGCTTCCAGGGGGCGGTCAGCCGCATTGGTGGAGCAAGCTTTGAAGACTTCCAGCAGGATCTGCTGAATCTGTCGAAACGTGCCTGGCTGGCGCGTAAGCCCATCAGCCAGGGCGGTCTGCTGAAATACGTCCACGGCGGCGAATACCACGCCTA  >  NC_000913/3356837‑3357108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A