Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F73 I1 R1	26	148.5	5206078	99.3%	5169635	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,500,177	C→A	pseudogene (615/1199 nt)	yjgX ←	KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,500,177	0	C	A	99.5%	656.9 / ‑5.5	181	pseudogene (615/1199 nt)	yjgX	KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (96/84); total (96/85)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

CGGCTAGGAGTTAAGGCTGTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGCGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAGCGC  >  NC_000913/4500040‑4500309

cGGCTAGGAGTTAAGGCTGTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGgaagaga                                                                                                                                    >  1:904780/1‑140 (MQ=255)

 ggCTAGGAGTTAAGGCTGTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGgaagagaa                                                                                                                                   >  1:554315/1‑140 (MQ=255)

 ggCTAGGAGTTAAGGCTGTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGgaagagaa                                                                                                                                   >  2:2523089/1‑140 (MQ=255)

  gCTAGGAGTTAAGGCTGTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGgaagagaag                                                                                                                                  >  2:1831773/1‑140 (MQ=255)

   cTAGGAGTTAAGGCTGTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGgaagagaagc                                                                                                                                 >  2:1615022/1‑140 (MQ=255)

      ggAGTTAAGGCTGTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGc                                                                                                                              <  2:2548714/140‑1 (MQ=255)

            aaGGCTGTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCt                                                                                                                        >  1:1218212/1‑140 (MQ=255)

             aGGCTGTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCtt                                                                                                                       >  1:647113/1‑140 (MQ=255)

               gCTGTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGa                                                                                                                     <  2:1577328/140‑1 (MQ=255)

                 tGTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGc                                                                                                                              <  1:129211/129‑1 (MQ=255)

                  gTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGa                                                                                                                  >  1:580596/1‑140 (MQ=255)

                  gTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGa                                                                                                                  >  1:2528480/1‑140 (MQ=255)

                  gTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGa                                                                                                                  >  1:170636/1‑140 (MQ=255)

                  gTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCATGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGa                                                                                                                  >  1:171676/1‑140 (MQ=255)

                   tCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGaa                                                                                                                 >  2:233372/1‑140 (MQ=255)

                   tCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGaa                                                                                                                 >  1:2285560/1‑140 (MQ=255)

                          gATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCa                                                                                                          <  1:1039286/140‑1 (MQ=255)

                          gATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCa                                                                                                          <  2:2391412/140‑1 (MQ=255)

                                    agaACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCAt                                                                                                <  2:647113/140‑1 (MQ=255)

                                     gaACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATc                                                                                               >  2:935961/1‑140 (MQ=255)

                                     gaACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATc                                                                                               >  2:1749437/1‑140 (MQ=255)

                                      aaCCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCa                                                                                              <  1:2126114/140‑1 (MQ=255)

                                      aaCCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCa                                                                                              <  1:673475/140‑1 (MQ=255)

                                      aaCCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCa                                                                                              <  2:554315/140‑1 (MQ=255)

                                      aaCCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCa                                                                                              <  2:1118536/140‑1 (MQ=255)

                                      aaCCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCa                                                                                              <  1:233372/140‑1 (MQ=255)

                                      aaCCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCa                                                                                              <  1:2420767/140‑1 (MQ=255)

                                        cccATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGa                                                                                            <  1:750710/140‑1 (MQ=255)

                                        cccATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGa                                                                                            <  2:2114284/140‑1 (MQ=255)

                                        cccATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGa                                                                                            <  1:1615022/140‑1 (MQ=255)

                                        cccATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGa                                                                                            <  1:1334328/140‑1 (MQ=255)

                                          catcatGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAg                                                                                          <  2:366659/140‑1 (MQ=255)

                                             catGTGCAGTAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCaa                                                                                       <  2:2315229/140‑1 (MQ=255)

                                              atGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAAt                                                                                      >  1:1126676/1‑140 (MQ=255)

                                               tgtgCAGGAAAATTATGTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGTCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATa                                                                                     >  2:2292934/1‑140 (MQ=255)

                                                gtgCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATaa                                                                                    >  2:1526855/1‑140 (MQ=255)

                                                gtgCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATaa                                                                                    >  2:2036336/1‑140 (MQ=255)

                                                 tgCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAAc                                                                                   >  1:2546320/1‑140 (MQ=255)

                                                  gCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTcc                                                                                                    >  2:1070297/1‑122 (MQ=255)

                                                  gCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTcc                                                                                                    <  1:1070297/122‑1 (MQ=255)

                                                   cAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACag                                                                                 >  1:825593/1‑140 (MQ=255)

                                                   cAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACag                                                                                 <  1:1749437/140‑1 (MQ=255)

                                                   cAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACag                                                                                 >  1:947105/1‑140 (MQ=255)

                                                   cAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACag                                                                                 >  1:2318556/1‑140 (MQ=255)

                                                    aGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACaga                                                                                <  2:581969/140‑1 (MQ=255)

                                                        aaaTTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATg                                                                            <  2:1477840/140‑1 (MQ=255)

                                                          aTTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCg                                                                          >  2:1161378/1‑140 (MQ=255)

                                                           ttATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCgt                                                                         >  1:1991968/1‑140 (MQ=255)

                                                           ttATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCgt                                                                         >  2:2485026/1‑140 (MQ=255)

                                                           ttATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCgt                                                                         >  1:1697860/1‑140 (MQ=255)

                                                                ttCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTc                                                                    >  1:2016781/1‑140 (MQ=255)

                                                                ttCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTc                                                                    >  1:1566451/1‑140 (MQ=255)

                                                                 tCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCa                                                                   >  1:2074474/1‑140 (MQ=255)

                                                                  cGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCAt                                                                  >  2:398001/1‑140 (MQ=255)

                                                                   ggAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATg                                                                 >  1:2187978/1‑140 (MQ=255)

                                                                   ggAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATg                                                                 >  2:103790/1‑140 (MQ=255)

                                                                    gagaGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGc                                                                <  2:1304348/140‑1 (MQ=255)

                                                                         gATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGc                                                                           <  1:1430676/124‑1 (MQ=255)

                                                                            gTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAg                                                        >  1:2000434/1‑140 (MQ=255)

                                                                                   ccAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCt                                                 >  2:2421185/1‑140 (MQ=255)

                                                                                   ccAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCt                                                 >  2:1481725/1‑140 (MQ=255)

                                                                                   ccAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCt                                                 >  2:977974/1‑140 (MQ=255)

                                                                                     aGCGCACGTTCTGTTTCATGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTg                                               <  2:171676/140‑1 (MQ=255)

                                                                                       cgcACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGAt                                             >  2:737159/1‑140 (MQ=255)

                                                                                       cgcACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGAt                                             <  1:1814099/140‑1 (MQ=255)

                                                                                       cgcACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGAt                                             >  1:1844371/1‑140 (MQ=255)

                                                                                       cgcACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGAt                                             <  1:19157/140‑1 (MQ=255)

                                                                                       cgcACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGAt                                             <  1:453154/140‑1 (MQ=255)

                                                                                       cgcACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGAt                                             >  2:189619/1‑140 (MQ=255)

                                                                                       cgcACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTC‑TGCTCCCCCAGTTTTCCTTGAtt                                            >  2:326592/1‑140 (MQ=255)

                                                                                         cACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGAt                                             >  1:2095586/1‑138 (MQ=255)

                                                                                         cACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTg                                           >  2:1984961/1‑140 (MQ=255)

                                                                                           cGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGa                                         <  2:730471/140‑1 (MQ=255)

                                                                                               cTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATc                                     <  1:2427394/140‑1 (MQ=255)

                                                                                                   ttCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATccacc                                 <  2:2546320/140‑1 (MQ=255)

                                                                                                     ccTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCAt                               <  2:2016781/140‑1 (MQ=255)

                                                                                                      cTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCAtg                              >  2:2076314/1‑140 (MQ=255)

                                                                                                       tGTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGAt                                             <  1:1009696/124‑1 (MQ=255)

                                                                                                        gTAACAACAGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCAtgtg                            >  2:862706/1‑140 (MQ=255)

                                                                                                          aaaaacaGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCt                          >  1:348117/4‑140 (MQ=255)

                                                                                                            caacaGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGata                                       <  1:386832/125‑1 (MQ=255)

                                                                                                                aGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATcca                                   >  2:2130130/1‑125 (MQ=255)

                                                                                                                aGTTTGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGAGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATcc                    >  2:1288915/1‑140 (MQ=255)

                                                                                                                   ttGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGc                 >  1:808682/1‑140 (MQ=255)

                                                                                                                    tGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCt                >  2:766813/1‑140 (MQ=255)

                                                                                                                    tGTCATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATCCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCt                >  1:479438/1‑140 (MQ=255)

                                                                                                                       cATCTGTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCtttt             >  2:2443334/1‑140 (MQ=255)

                                                                                                                            gTTTTACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTtgctgc        >  1:2068293/1‑140 (MQ=255)

                                                                                                                             ttttACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGcc       <  2:170636/140‑1 (MQ=255)

                                                                                                                             ttttACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGcc       <  2:1913821/140‑1 (MQ=255)

                                                                                                                             ttttACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGcc       <  2:808682/140‑1 (MQ=255)

                                                                                                                               ttACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAg     <  2:1697860/140‑1 (MQ=255)

                                                                                                                               ttACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAg     <  2:2187978/140‑1 (MQ=255)

                                                                                                                               ttACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAg     >  2:2148873/1‑140 (MQ=255)

                                                                                                                               ttACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAg     <  1:203671/140‑1 (MQ=255)

                                                                                                                                tACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAgc    >  1:363107/1‑140 (MQ=255)

                                                                                                                                tACGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAgc    >  2:1379627/1‑140 (MQ=255)

                                                                                                                                 aCGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAgcg   <  1:977974/140‑1 (MQ=255)

                                                                                                                                 aCGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAgcg   <  1:1481725/140‑1 (MQ=255)

                                                                                                                                  cGGGAAGAGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAgcgc  >  2:1421625/1‑140 (MQ=255)

CGGCTAGGAGTTAAGGCTGTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGCGAAGCTGCCTTTCTTGAGGAAAGTGGCATGCTCCGCATCAGAAGCAATAACAGAGATGCGTGTGTCATGCTCCCCCAGTTTTCCTTGATTGGATATCCACCATGTGCTGTATCCTGCTTTTGCTGCCAGCGC  >  NC_000913/4500040‑4500309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A