Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F73 I1 R1	33	77.0	2636344	99.1%	2612616	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,920,441	C→A	D150Y (GAT→TAT)	atpF ←	ATP synthase Fo complex subunit b

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,920,441	0	C	A	100.0%	121.0 / NA	36	D150Y (GAT→TAT)	atpF	ATP synthase Fo complex subunit b
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (21/15); total (21/15)

AGCATGTCCTGCCAGCGTTCTACACTTTGGTGTTCGACGGCAAAGTCAAAAGCTGCTTTGGCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATCCACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGCCAGGATAGCAACTTGCTTACGCAGCTCTTCACGGGCACGTTTACGCTCGGCTTCAATTTCC  >  NC_000913/3920309‑3920568

aGCATGTCCTGCCAGCGTTCTACACTTTGGTGTTCGACGGCAAAGTCAAAAGCTGCTTTGGCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATg                                                                                                                          <  2:958400/140‑1 (MQ=255)

aGCATGTCCTGCCAGCGTTCTACACTTTGGTGTTCGACGGCAAAGTCAAAAGCTGCTTTGGCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATg                                                                                                                          <  2:953578/140‑1 (MQ=255)

  cATGTCCTGCCAGCGTTCTACACTTTGGTGTTCGACGGCAAAGTCAAAAGCTGCTTTGGCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTc                                                                                                                        >  1:1089528/1‑140 (MQ=255)

   aTGTCCTGCCAGCGTTCTACACTTTGGTGTTCGACGGCAAAGTCAAAAGCTGCTTTGGCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCg                                                                                                                       >  2:490042/1‑140 (MQ=255)

               cGTTCTACACTTTGGTGTTCGACGGCAAAGTCAAAAGCTGCTTTGGCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCt                                                                                                           >  2:1070019/1‑140 (MQ=255)

               cGTTCTACACTTTGGTGTTCGACGGCAAAGTCAAAAGCTGCTTTGGCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCt                                                                                                           >  2:1065233/1‑140 (MQ=255)

                gTTCTACACTTTGGTGTTCGACGGCAAAGTCAAAAGCTGCTTTGGCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCtt                                                                                                          >  1:485770/1‑140 (MQ=255)

                         tttGGTGTTCGACGGCAAAGTCAAAAGCTGCTTTGGCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACgg                                                                                                 >  2:188630/1‑140 (MQ=255)

                           tGGTGTTCGACGGCAAAGTCAAAAGCTGCTTTGGCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGaa                                                                                               <  1:1292769/140‑1 (MQ=255)

                           tGGTGTTCGACGGCAAAGTCAAAAGCTGCTTTGCCGTAGGGGCGAGCTACCGTAATAAATTCAGAAATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACTGaa                                                                                               >  2:1259149/1‑140 (MQ=255)

                            ggTGTTCGGCTGCAAAGTCAAAAGCTGCTTTGGCATAGGGGTGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTTGCTGTTAGCAGCTTCATCCACGGAAc                                                                                              <  2:520834/140‑1 (MQ=255)

                              tgtTCGACGGCAAAGTCAAAAGCTGCTTTGGCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCa                                                                                                        <  1:1266222/128‑1 (MQ=255)

                              tgtTCGACGGCAAAGTCAAAAGCTGCTTTGGCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCa                                                                                                        >  2:1266222/1‑128 (MQ=255)

                               gtTCGACGGCAAAGTCAAAAGCTGCTTTGGCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGtt                                                                                           <  2:485770/140‑1 (MQ=255)

                                ttCGACGGCAAAGTCAAAAGCTGCTTTGGCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTc                                                                                          >  1:37003/1‑140 (MQ=255)

                                                  aGCTGCTTTGGCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGcc                                                                        <  2:37003/140‑1 (MQ=255)

                                                      gctTTGGCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCa                                                                    <  1:1070019/140‑1 (MQ=255)

                                                       ctTTGGCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCaa                                                                   >  1:164977/1‑140 (MQ=255)

                                                        tttGGCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAAc                                                                  <  2:164977/140‑1 (MQ=255)

                                                          tGGCGTAGGGGCGAGCTACCGTAATAAATTCAGACACCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAg                                                                <  1:55818/140‑1 (MQ=255)

                                                           ggCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGc                                                               <  2:824209/140‑1 (MQ=255)

                                                           ggCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGc                                                               <  2:979474/140‑1 (MQ=255)

                                                           ggCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGc                                                               <  1:188630/140‑1 (MQ=255)

                                                                       aGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGCCAGGATAGCAAc                                                   >  2:1239122/1‑140 (MQ=255)

                                                                       aGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGCCAGGATAGCAAc                                                   >  2:818741/1‑140 (MQ=255)

                                                                                       tCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGCCAGGATAGCAACTTGCTTACGCAGctct                                   >  1:1024112/1‑140 (MQ=255)

                                                                                              tCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGCCAGGATAGCAACTTGCTTACGCAGCTCTTCACggg                            <  2:1089528/140‑1 (MQ=255)

                                                                                               cAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGCCAGGATAGCAACTTGCTTACGCAGCTCTTCACGGGc                           >  2:295852/1‑140 (MQ=255)

                                                                                                   ccctccctCCTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGCCAGGATAGCAACTTGCTTACGCAGCTCTTCACGGGCACGt                       >  2:956973/1‑140 (MQ=255)

                                                                                                       ccctccTTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGCCAGGATAGCAACTTGCTTACGCAGCTCTTCACGGGCACGTTTAc                   >  1:1111332/1‑140 (MQ=255)

                                                                                                          tcctTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGCCAGGATAGCAACTTGCTTACGCAGCTCTTCACGGGCACGTTTACGCt                <  1:1065233/140‑1 (MQ=255)

                                                                                                          ccctTACAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGCCAGGATAGCAACTTGCTTACGCAGCTCTTCACGGGCACGTTTACGCt                <  1:1259149/139‑1 (MQ=255)

                                                                                                                cAGTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGCCAGGATAGCAACTTGCTTACGCAGCTCTTCACGGGCACGTTTACGCTCGGCtt          <  1:755277/140‑1 (MQ=255)

                                                                                                                  gTTCAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGCCAGGATAGCAACTTGCTTACGCAGCTCTTCACGGGCACGTTTACGCTCGGCTTCa        >  1:420355/1‑140 (MQ=255)

                                                                                                                     cAGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGCCAGGATAGCAACTTGCTTACGCAGCTCTTCACGGGCACGTTTACGCTCGGCTTCAAtt     >  1:108618/1‑140 (MQ=255)

                                                                                                                      aGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGCCAGGATAGCAACTTGCTTACGCAGCTCTTCACGGGCACGTTTACGCTCGGCTTCAAttt    >  1:1294201/1‑140 (MQ=255)

                                                                                                                      aGCGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGCCAGGATAGCAACTTGCTTACGCAGCTCTTCACGGGCACGTTTACGCTCGGCTTCAAttt    >  1:1099028/1‑140 (MQ=255)

                                                                                                                        cGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGCCAGGATAGCAACTTGCTTACGCAGCTCTTCACGGGCACGTTTACGCTCGGCTTCAATTTcc  >  2:171858/1‑140 (MQ=255)

                                                                                                                        cGACAAGTTTATACACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGCCAGGATAGCAACTTGCTTACGCAGCTCTTCACGGGCACGTTTACGCTCGGCTTCAATTTcc  >  1:577103/1‑140 (MQ=255)

AGCATGTCCTGCCAGCGTTCTACACTTTGGTGTTCGACGGCAAAGTCAAAAGCTGCTTTGGCGTAGGGGCGAGCTACCGTAATAAATTCAGACATCAGCCCCTCCCTCCTTACAGTTCAGCGACAAGTTTATCCACGATGTCGCTGTTAGCAGCTTCATCCACGGAACGTTCGATGATCTTCTCGGCGCCAGCAACAGCCAGGATAGCAACTTGCTTACGCAGCTCTTCACGGGCACGTTTACGCTCGGCTTCAATTTCC  >  NC_000913/3920309‑3920568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A