Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F73 I1 R1	33	77.0	2636344	99.1%	2612616	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,332,933	C→A	A114S (GCC→TCC)	basS ←	sensory histidine kinase BasS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,332,933	0	C	A	98.7%	257.1 / ‑3.1	76	A114S (GCC→TCC)	basS	sensory histidine kinase BasS
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (35/40); total (35/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01

GCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTCAGGCTGACCATAAAGACGCCGGGGACAATCAGG  >  NC_000913/4332797‑4333069

gCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAAt                                                                                                                                       <  2:107032/140‑1 (MQ=255)

gCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAAt                                                                                                                                       <  1:84930/140‑1 (MQ=255)

gCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAAt                                                                                                                                       <  1:443567/140‑1 (MQ=255)

      ggCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGt                                                                                                                                 >  2:1247198/1‑140 (MQ=255)

      ggCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGt                                                                                                                                 >  1:1114972/1‑140 (MQ=255)

       gCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGtt                                                                                                                                <  2:188335/140‑1 (MQ=255)

        cGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTa                                                                                                                               >  2:5994/1‑140 (MQ=255)

        cGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTa                                                                                                                               <  1:271651/140‑1 (MQ=255)

          ttCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAg                                                                                                                             >  1:19811/1‑140 (MQ=255)

                 ttCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTcgg                                                                                                                      >  2:467643/1‑140 (MQ=255)

                   cGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTcggcg                                                                                                                    >  1:1002866/1‑140 (MQ=255)

                            cGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCt                                                                                                           <  1:1118283/140‑1 (MQ=255)

                                aGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCa                                                                                                       >  1:465065/1‑140 (MQ=255)

                                  cGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGc                                                                                                     <  2:465065/140‑1 (MQ=255)

                                       aacaacCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCttt                                                                                                >  2:198302/1‑140 (MQ=255)

                                       aacaacCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCttt                                                                                                >  2:1197591/1‑140 (MQ=255)

                                          aacCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTg                                                                                             <  1:881012/140‑1 (MQ=255)

                                               ttCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCt                                                                                        >  2:276189/1‑140 (MQ=255)

                                                tCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTc                                                                                       >  1:1231940/1‑140 (MQ=255)

                                                 cGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTcc                                                                                      >  1:1193910/1‑140 (MQ=255)

                                                      tCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAg                                                                                 >  1:596492/1‑140 (MQ=255)

                                                      tCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAg                                                                                 <  2:1086985/140‑1 (MQ=255)

                                                      tCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAg                                                                                 >  1:1036171/1‑140 (MQ=255)

                                                        cAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAgcg                                                                               <  1:369461/140‑1 (MQ=255)

                                                           cGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAgcgggc                                                                            >  1:26716/1‑140 (MQ=255)

                                                           cGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAgcgggc                                                                            >  2:822802/1‑140 (MQ=255)

                                                            gTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAgcgggcg                                                                           >  1:160928/1‑140 (MQ=255)

                                                             tGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAgcgggcgg                                                                          >  2:4921/1‑140 (MQ=255)

                                                               cTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGt                                                                        >  1:94098/1‑140 (MQ=255)

                                                                tGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTg                                                                       >  2:954423/1‑140 (MQ=255)

                                                                tGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTg                                                                       >  1:603271/1‑140 (MQ=255)

                                                                tGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTg                                                                       >  1:808821/1‑140 (MQ=255)

                                                                    cAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATgc                                                                   <  1:198302/140‑1 (MQ=255)

                                                                        cGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATgcggcg                                                               <  1:4921/140‑1 (MQ=255)

                                                                          aCTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTa                                                             <  2:160928/140‑1 (MQ=255)

                                                                           cTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTAc                                                            <  2:444917/140‑1 (MQ=255)

                                                                            tgcccAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGCGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCCGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGACGCGGCGTAcc                                                           >  2:1305223/4‑140 (MQ=255)

                                                                            tGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTg                                                                       <  2:797967/128‑1 (MQ=255)

                                                                            tGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTg                                                                       >  1:797967/1‑128 (MQ=255)

                                                                            tGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTAcc                                                           >  1:216161/1‑140 (MQ=255)

                                                                            tGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGGGATGCGGCGTAcc                                                           >  2:796925/1‑140 (MQ=255)

                                                                              aCCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGc                                                         <  2:26716/140‑1 (MQ=255)

                                                                                 aGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTg                                                      >  1:1066889/1‑140 (MQ=255)

                                                                                   aTCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGAt                                                    <  2:808821/140‑1 (MQ=255)

                                                                                   aTCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGAt                                                    <  2:383527/140‑1 (MQ=255)

                                                                                   aTCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGAt                                                    <  1:5994/140‑1 (MQ=255)

                                                                                   aTCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGAt                                                    <  2:596492/140‑1 (MQ=255)

                                                                                   aTCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGAt                                                    >  2:597437/1‑140 (MQ=255)

                                                                                   aTCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGAt                                                    <  2:216161/140‑1 (MQ=255)

                                                                                     cGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAg                                                  <  1:276189/140‑1 (MQ=255)

                                                                                       tttAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCa                                                <  1:137435/140‑1 (MQ=255)

                                                                                       tttAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCa                                                <  2:603271/140‑1 (MQ=255)

                                                                                          aaCGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGAt                                             >  1:1002094/1‑140 (MQ=255)

                                                                                          aaCGCCGAAACCACCGCTTCGATTTCGAGGGCGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGGCTCCAGCTCTTTTTGTAGCTCCGCCAGCGGGCGGGTGCTGCGGCGTACCGCCTGATGGCAGAt                                             >  2:617519/1‑140 (MQ=255)

                                                                                             gCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATaaa                                          >  1:1044019/1‑140 (MQ=255)

                                                                                             gCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGGGATGCGGCGTACCGCCTGATAGCAGATaaa                                          >  2:951250/1‑140 (MQ=255)

                                                                                               cGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATa                                        <  2:94098/140‑1 (MQ=255)

                                                                                                 aaaCCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGc                                      >  1:107603/1‑140 (MQ=255)

                                                                                                  aaCCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCg                                     <  2:59798/140‑1 (MQ=255)

                                                                                                    ccaccGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTc                                   <  2:549381/140‑1 (MQ=255)

                                                                                                       ccGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTCAgg                                <  2:362830/140‑1 (MQ=255)

                                                                                                       ccGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTCAgg                                <  2:1066889/140‑1 (MQ=255)

                                                                                                       ccGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTCAgg                                <  1:597437/140‑1 (MQ=255)

                                                                                                        cGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTCAGGc                               >  2:455924/1‑140 (MQ=255)

                                                                                                        cGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTCAGGc                               <  2:469034/140‑1 (MQ=255)

                                                                                                        cGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTCAGGc                               <  1:1197591/140‑1 (MQ=255)

                                                                                                        cGCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTCAGGc                               <  1:1247198/140‑1 (MQ=255)

                                                                                                         gCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTCAGGCt                              <  2:1002866/140‑1 (MQ=255)

                                                                                                         gCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTCAGGCt                              <  1:1305223/140‑1 (MQ=255)

                                                                                                         gCTTCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTCAGGCt                              <  1:112320/140‑1 (MQ=255)

                                                                                                           ttCGATTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTCAGGCTGa                            >  1:237971/1‑140 (MQ=255)

                                                                                                               aTTTCGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGCTAGCAGATAAATAGCCTCAGGCCGACCac                        >  2:971401/1‑139 (MQ=255)

                                                                                                                   cGAGGGTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTCAGGCTGACCATAAAg                    <  2:251701/140‑1 (MQ=255)

                                                                                                                        gTGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTCAGGCTGACCATAAAGACGcc               <  1:954423/140‑1 (MQ=255)

                                                                                                                         tGGCGCTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTCAGGCTGACCATAAAGACGCCg              <  1:971401/140‑1 (MQ=255)

                                                                                                                           gcgcTGTGAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTCAGGCTGACCATAAAGACGCCggg            <  1:786230/140‑1 (MQ=255)

                                                                                                                                 tgAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTCAGGCTGACCATAAAGACGCCGGGGACAAt      >  2:591882/1‑140 (MQ=255)

                                                                                                                                  gAATGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTCAGGCTGACCATAAAGACGCCGGGGACAATc     <  2:19811/140‑1 (MQ=255)

                                                                                                                                     tGGAAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTCAGGCTGACCATAAAGACGCCGGGGACAATCAgg  <  2:1231940/140‑1 (MQ=255)

GCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGGCGTTAAGTTGTCGGCGGTGCGCGCTTCCAGCTCTTTTTGCAGCTCCGCCAGCGGGCGGGTGATGCGGCGTACCGCCTGATAGCAGATAAATAGCGTCAGGCTGACCATAAAGACGCCGGGGACAATCAGG  >  NC_000913/4332797‑4333069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A